WEL COME FIELD DETERMINATION PRESERVATION TECHNIQUES

WEL COME

FIELD DETERMINATION, PRESERVATION TECHNIQUES

LABORATORY PREPARATION FOR SAMPLING SAMPLERS SAMPLE CONTAINERS REAGENT SOLUTIONS INSTRUMENTS FIELD KIT GLASSWARES

CHECK LIST FOR COLLECTION OF SAMPLE SAMPLING TOUR PROGRAMME AREA MAP ICE BOX FILLED WITH ICE DO SAMPLER BOD BOTTLE SAMPLE CONTAINERS SAMPLE PRESERVATIVES

CHECK LIST FOR COLLECTION OF SAMPLE TISSUE PAPERS SAMPLE ID FORMS FIELD NOTE BOOK SOAP AND TOWEL DRINKING WATER TRANSPORT ARRANGEMENT SAMPLING SITE LOCATION MAP WEIGHTED BOTTLE SAMPLER

CHECK LIST FOR COLLECTION OF SAMPLE ROPE SAMPLE CONTAINERS DO FIXING AND TITRATION CHEMICALS & GLASSWARES THERMOMETER FIELD KIT, AS REQUIRED LABELS FOR SAMPLE CONTAINERS PEN, PENCIL, MARKER, MATCH BOX, TORCH, KNIFE, GLOVES & EYE PROTECTION

Sample Collection RECOMMENDED SAMPLE COLLECTION DEVICES AND TYPES SR. NO PARAMETER DEVICE 01 D. O. 02 03 CONTAINER QUANTITY PRESERVATION AT SITE DO BOD BOTTLE SAMPLER 300 ml DO SETTING AT SITE GENERAL PVC CAN 2000 ml NONE COLIFORMS MPN BOTTLE STERILIZED BOTTLE 500 ml COOLING AT 4 0 C

Sample Collection SAMPLE COLLECTION DEVICE AND TYPE 1) D. O. SAMPLER WITH 300 ml BOD BOTTLE 2) ( TO AVOID AIRATION) 3) 2) DEPTH INTEGRATING SAMPLER= D-74 4) ( DEPTH UPTO 4. 72 m) 5) 3) DEPTH INTEGRATING SAMPLER= P-74 6) ( DEPTH MORE THAN 4. 72 m)

DO SAMPLER

DO SAMPLER 20 -30 CM BELOW THE SURFACE

SAMPLING FOR D. O. WATER LEVEL 20 -30 CM BELOW THE SURFACE

D-74 SAMPLER FOR DEPTH UP TO 4. 72 m

DEPTH INTEGRATING SAMPLER D-74 SALIENT FEATURESCAST IRON STREAM – LINE BODY BOTTOM HINGED HEAD BOTTEL FITS IN TO THE CHAMBER TAIL VANES 4. 8 mm NOZZELS-6. 4 mm, 4. 8 mm AND 3. 2 mm AIR OUTLET VELOCITY – 2. 4 m/s DEPTH – 4. 72 m

P-61 SAMPLER FOR DEPTH MORE THAN 4. 72 m

POINT INTEGRATING SAMPLER P-61 SALIENT FEATURES BRONZE CASTING – STREA LINED BOTTOM HINGED HEAD TAIL VANES 4. 8 mm NOZZEL PRESSURE EQUILISING CHAMBER ROTARY SOLENOID ACTIVATED VALVE 24 VOLTS CURRENT COLLECTING SAMPLES AT SELECTED POINT USED AS DEPTH INTEGRATING SAMPLERIN DEEP STREAM(DEPTH MORE THAN 4. 72 m)

USDH-48 SAMPLER USED WHERE : V x D < 1

USDH-48 SAMPLER SALIENT FEATURES LIGHT WEIGHT, SMALL & EASY TO OPERATE USED WHERE VELOCITY x DEPTH < 1 AIR OUTLET ALLUMINIUM CASTING WEIGHT 2 KG NOZZEL – 6. 30 mm, 4. 80 mm, 3. 15 mm COLLECTING SAMPLES WITHIN 9 cm OF STREAM BED REQUIRED CABLE AND FISHWEIGHT

SELECTION OF ANALYTICAL PARAMETERS CONSIDERING WATER YEAR FROM JUNE TO MAY PARAMETERS TESTED IN LAB (AS PER TYPE OF LOCATION) BASELINE – Ist-30– Balance – 20 TREND - Ist-30– Balance – 20 TREND/FLUX -Ist-30– Balance – 20 RESERVIOR/DAM -Ist-32– Balance – 22

CLIPS

SAMPLING FOR DO

SAMPLING FOR BACTERIOLOGICAL SAMPLE

WATER TESTING KIT ( FIELD KIT)

FIELD TEST MEASUREMENT OF FIELD PARAMETERS COLOUR, ODOUR, TEMPARATURE, EC, p. H, & DO ARE FIELD PARAMETERS AND ARE TO BE ANALYSED AS SOON AS SAMPLE IS COLLECTED MEASUREMENTS OF THESE PARAMETERS CAN BE MADE BY USING FIELD KITS. THIS IS BEST OPTION ANOTHER OPTION- NEARBY LEVEL-1 LAB D. O. SAMPLE MUST BE FIXED IMMIDIETLY AFTER COLLECTION OF SAMPLE TEMPARATURE MUST BE MEASURED AT THE SITE ONLY

FIELD TEST FILLING AN ID FORM

“SAMPLE ID FORM”

FIELD TEST COLOUR CODES 1) LIGHT BROWN 2) BROWN 3) DARK BROWN 4) LIGHT GREEN 5) GREEN 6) DARK GREEN 7) CLEAR 8) OTHER SPECIFY

FIELD TEST ODOUR CODE 1) ODOUR FREE 2) ROTTEN EGGS 3) BURNT SUGAR 4) SOAPY 5) FISHY 6) SEPTIC 7) AROMATIC 8) CHLORINIOUS 9) ALCOHOLIC 10)UNPLEASENT

FIELD TEST TEMPARATURE IN DEGREE CELCIOUS BY USING THERMOMETER OR TESTING KIT

FIELD TEST p. H INDICATES CONCENTRATION OF H AND OH IONS INDICATES ACIDIC OR ALKALINE NATURE OF WATER IS ACIDIC WHEN p. H < 7 WATER IS ALKALINE WHEN p. H > 7 THE p. H of DISTILLED WATER IS 7

FIELD TEST MEASUREMENT OF p. H LITMUS PAPER- • ACIDIC – BLUE LITMUS PAPER TURNS IN TO RED • ALKALINE – RED LITMUS PAPER TURNS IN TO BLUE • NUTRAL – NO CHANGE IN ANY PAPER • BY USING p. H PAPER HAVING VALUES OF VARIOUS RANGES 4 -11 (UNIVERSAL), 1 -14, 1 -10 etc.

FIELD TEST MEASUREMENT OF p. H COMARATOR -DISC OF p. H 10 ml TEST TUBE UNIVERSEL INDICATOR

FIELD TEST MEASUREMENT OF p. H ELECTRICAL p. H METER OR PENTYPE 1) REQUIRED STOCK SOLUTION 2) BUFFER SOLUTION OF 4, 7, & 9. 2 3) CALIBRATION OF INSTRUMENT BY USING BUFFER SOLUTION TO TAKE OBSERVATION

FIELD TEST DISSOLVED OXYGEN 1) GLASS WARES-D. O. BOTTLE, PIPPETES, BURRETS & CONICAL FLASK etc. 2) CHEMICALS( BUFFER SOLUTION) 3) 1) MANGNESE SULPHATE, 4) 2)ALKALI IODIDE AZIDE REAGENT 5) 3) STARCH SOLUTION 6) 4) SODIUM THIOSULPHATE 7) 5) CONC. H 2 SO 4

FIELD TEST ELECTRICAL CONDUCTIVITY EC AT FIELD – EC METER -CALIBRATION OF INSTRUMENT -RINSE CONDUCTIVITY CELL – 0. 01 M KCL MEASURE RESISTANCE, TEMPARATURE ADUST METER TO 1412 Micro mhos. /cm COMPUTE THE CELL CONSTANT Kc RINSE CELL IN SAMPLE CALCULATE EC

FIELD DETERMINATION

FIELD DETERMINATION MEASUREMENT OF D. O.

MEASUREMENT OF D. O. RESULT OF D. O. IS 4. 74

SAMPLE LABELLING SAMPLE CODE NUMBER DATE AND TIME OF SAMPLING SOURCE AND TYPE OF SAMPLE PRETREATMENT OR PRESERVATION ANY SPECIAL NOTE FOR ANALYST SAMPLERS NAME , etc.

SAMPLE LABEL HYDROLOGY PROJECT(SW) MAHARASHTRA WATER QUALITY LAB LEVEL-II @ NASHIK Name of Location: ID No. River: Tal: Dist: Date of sampling: Sampling Time : Sample collected by: Sample received by: Sr. No. in Sample entry register:

SAMPLE PRESERVATION AND TRANSPORTATION

Water Quality Parameters Recommended Maximum Storage Times.

SAMPLE PRESERVATION AND TRANSPORTATION Water Quality Parameters Recommended Maximum Storage Times. Recommended Required Temperature Immediate 7 days Conductivity 28 days p. H Immediate Dissolved Oxygen 8 hours Total Dissolved Solids Nutrients Parameter Suspended Solid Parameter Group General 7 days Ammoniacal Nitrogen 7 days 28 days Total Oxidized Nitrogen --- 28 days Total Phosphorus ---

Water Quality Parameters Recommended Maximum Storage Times. Organic Matter COD 7 days 28 days BOD 6 hours 48 hours Major Ions Sodium 6 months Potassium 6 months Calcium 6 months Magnesium 6 months Carbonates and Bicarbonates Chloride 24 hours 14 days 28 days Sulphate 28 days

Water Quality Parameters Recommended Maximum Storage Times. Silica 28 days Fluoride 28 days Boron 28 days 6 months Cadmium 3 6 months Mercury 3 28 days Zinc 3 6 months Pesticide (Indicator 7 days Synthetic Detergents --- Organic Solvents --- Phenols 3 --- 28 days Micro bio Total Coliforms 8 hours 24 hours Biological Chlorophyll ’a’ 30 days ----- Other Inorganic Metals Organics

SAMPLE TRANSPORTATION

SAMPLE TRANSPORTATION WITHIN 24 HOURS TO CONCERN LABORATORY USE ICE BOX ( 40 C DARK) DURING TRANSPORTATION FOR MPN SAMPLES

ICE BOX ( 40 C DARK)

ANALYSIS OF SAMPLES IN THE LABORATORY

MPN TEST

CHEMICAL ANALYSIS

ANALYSIS FOR IONS BY AAS

ANALYSIS FOR IONS BY AAS