Varian 940 -LC Analytical to Prep HPLC 1

Varian 940 -LC Analytical to Prep HPLC 1

Varian 940 -LC Silver Bullets • The 940 -LC one instrument – many uses: – High performance automated Analytical HPLC – High performance semi-preparative HPLC – High performance prep LC 2

Varian 940 -LC Silver Bullets • Flexible and capable – Wide flow rate from 0. 1 ml/min to 200 ml/min – Dual path-length flowcells for extended Abs – One Injector, analytical or prep volumes (5 ul to 4. 5 ml) • Superb fraction collecting – Galaxie real-time peak detection – Practical collect and inject – Much Easier interface, it does what you tell it! – Cleaner UI 3

Goals of the 940 -LC Analytical to Prep • Aim • Become market leader in this $180 M segment – Double market share • Market • Continue to focus on Pharma / Bio. Pharma • Academic • Strategy • Deliver innovation in hardware, consumables, software and services 4

Varian 940 -LC Instrument Overview • Factory tested, pre-plumbed instrument: – Plug and play operation • High performance semi-preparative HPLC • Gradient Analytical HPLC • Autoprep System – Choice of UV or PDA detector • Dual path flow cell for unmatched range and scalability – No compromise performance for prep and analytical – switch with a key stroke – no plumbing or hardware changes needed! – The most flexible analytical to prep HPLC available 5

Varian 940 -LC Instrument Overview • Scale-up Module for automated methods transfer from analytical to semi-prep, or switching from Analytical to Prep work • Versatile and robust solvent-delivery system • New loopless autosampler – wide volume range • NEW Fraction collection with – Complete range of collection modes such as slope, threshold and time slicing – Wide range of vessels, including autosampler rack • Comprehensive range of columns and consumables • More productivity via Galaxie. TM software – Valve automation for genuine unattended operation 6 – NEW direct control interface

Varian 940 -LC Versatility and Performance 1 ml/min • Easy to change pump heads – Versatile solvent delivery systems • Powerful pumps allow 4000 psi at 25 m. L/min operation for fast chromatography – Ideal for 4. 6 mm and 1” column – Able to use smaller 3 & 5 um particles columns at elevated pressures • Improves productivity – Save solvents and waste costs – Short run times save time 21 ml/min 7 – High resolution separations = high purity

Varian 940 -LC Pumps • Same range of accessories as PS 210/218 • 5 ml Head to 200 ml Head • Easily interchangeable pressure modules thru removable side panel. • Easy access to mixer • Self maintenance and versatility features have been completely retained with no compromises – integrated systems are notoriously difficult to maintain so this is Unique 8

Varian 940 -LC Pumps - FAB Feature Advantage Benefit Interchangeable Pump Heads (5 to 200 ml/min) Unrivalled flexibility for any application Economical and Convenient with Excellent Performance Open access for Easy for the user to ease of maintenance correct basic issues resulting in more uptime 9 Dependable Simple and system Durability Analytical to Prep on One system will do one System the work of two providing high performance flexibility Performance and Reliability at an Excellent Price/Performance ratio

Varian 940 -LC Autosampler • Prep version of the 920 -LC Sampler • 5 ul to 4. 5 ml Injection range – No necessity to combine a Manual injector – No need to dedicate injector to prep or analytical – One Sampler does all! – Cooled version • Loopless Design • Needle in path Design – No carryover – 2008 Technology – no changing loops 10

Varian 940 -LC Autosampler - FAB Feature Advantage Benefit Needle in Path Design No need for Injection Versatility, loops Economical No Carryover Performance One Injector, large or Simplifies the Performance small inject volumes system, no need to Accuracy and swap components for Seamless Simplicity analytical to prep One Autosampler, all No need for multiple rack formats injector options 11 Versatility Simplicity

Varian 940 -LC UV/Vis - PDA Detectors • High Sensitivity for Analytical applications • Extended absorbance – desensitised, for Prep Applications • All in One Cell! • NEW 4 x 0. 25 mm Cell low dead volume – Excellent performance at low flow rates for Analytical – Excellent Extended Absorbance for Prep 12

Varian 940 -LC Dual Pathlength Flowcell Absorbance Detector • Extended absorbance range without re-plumbing (<70 AU) • NEW 4 mm x 0. 25 mm • 4 mm x 0. 15 Super. Prep • Low dispersion • UV/Vis Detector • PDA Detector • Low noise ~ +10 m. AU • Low Drift ~ 1 m. AU/hr 13

Agilent 1200 Flowcells 14

Varian 940 -LC UV/Vis - PDA Detectors - FAB Feature NEW 4 x 0. 25 Low Volume Cell Optimised for Performance and Analytical to Prep, no Versatility and need to change Cells Dependable Tool Free maintenance Easy to change lamps and flow cells Simplicity Range of cells for ul/min to Litres/min 15 Advantage Benefit Fits all applications from Analytical to Benchtop Prep Versatility and Performance

Varian 440 -LC Fraction Collector • Fully controlled by software for foolproof collection by slope, level or timeslice • Known fluid path for accurate fraction collection • Software display of collected fractions and source injections • Reliable operation • Compatible with a wide range of containers – Belart and other optional racks – Microvial to 50 ml tube capacity – Autosampler rack compatibility provides practical collect and re-inject capabilities 16

Varian 440 -LC Fraction Collector • We chose not to collect Peak 3 • Peaks 1, 2, 4, 5, 6, 7 collect with exceptional accuracy • Excellent algorithm, and simple delay volume calculation 17 • Slope/Threshold based on real numbers!!

Varian 440 -LC Fraction Collector • Fraction Log shows peak location using colour coded rack assignment 18

Varian 440 -LC Fraction Collector - FAB Feature Benefit Modern Design, improved movement and hardware Significant improvement ergonomically on 701 Pleasing Appearance with Stylish fast Collector movement Simplified user interface 19 Advantage Easy to use, does what you ask it to do both manually and automated Simplicity and precious Dependability for customer fractions Inject/Collect and Re Collect into -inject A/sampler rack then re-insert into the Sampler for Fraction Purity Determination Provides Confidence in Fraction Puritys without the need to do any liquid transfers

Varian 445 -LC Scale-up Module Optimized flow paths for automated scale-up • Separate optimised flow paths for Prep and Analytical columns • Solvent selection valves for 4 solvents ( 2 per pump) • Integrated priming valves for easy solvent changes 20 • Two Integrated Push Button Manual valves for Analytical and prep Injections

Varian 445 -LC Scale Up Module( SUM) - FAB Feature 2 Solvent Selection valves for solvent switching and flexibility Can prime up 4 most Simplicity and popular solvents thus Dependability saving time on repriming Analytical and Prep Columns Positions for Automated Scale Up or Switching Seamless and simple Simplicity and transfer from an Versatility Analytical analysis to Prep Integrated Manual Valves which are dedicated for Analytical and Prep 21 Advantage Benefit Push button valve positioning with automatic return, eliminate operator error Even Simpler to use, eliminates user error improving Confidence in results

Varian 940 -LC Software Usability • Software designed for Prep users • Enhanced manual control • One page status and chromatogram • Real time fraction collection display • Advanced peak sensing for better fraction collection • Purity displays for absorbance detection – standard! • Documentation and audit trails for traceability and regulatory needs 22

Varian 940 -LC Configurations pump Modular inject detect SUM FC Isocratic 25 ml Manual UV/Vis NA NA Opt any Base Grad 25 ml Man inj Dual UV option Productive 1 Grad 25 ml Auto 5 ml +man Dual UV yes Auto 5 ml +man PDA yes pump manual Dual UV/Vis NA option Opt 50 Productive 2 Grad 25 ml Opt 50 Prep Grad 100 ml Opt 200 23

Varian 940 -LC Consumables • Columns designed for Prep users • From 3 to 10 mm media • Wide range of C 4 to C 18 • Silica and Polymer media • From 2 mm to 2” ID • Unique solutions for dedicated prep with load and lock systems • Matched Analytical to Prep columns and packing's to assure smooth scale up • Solutions for hardware, software and consumables 24

Varian 940 -LC Summary The 940 -LC one instrument – many uses – High performance automated Analytical HPLC – High performance semi-preparative HPLC • Versatility – Pumping system able to operate at analytical flow rates and scalable up to prep flows – Dual path-length flow cell technology for unmatched range – Scale-up Module for automated methods transfer – Advanced fraction collection with full software control • Complete range of columns and consumables 25 • Prep enhanced Galaxie TM software and valve automation for genuine unattended operation

Conclusion for 900 Series • Compact and Stylish Integrated Systems • Retain flexibility and maintenance features • Top Tier hardware performance • Top Tier User Interface – pleasant experience • Simplicity in a box – Install , Use and Maintain • 1 st Truly Integrated HPLC’s on the Market 26

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