Varian 900 -LC Series i HPLC What

Varian 900 -LC Series i. HPLC

What is the i. HPLC? • 2 models 920 -LC and 940 -LC • Compact, stylish, automated analytical and semi prep HPLC • Pre-plumbed and factory tested for the highest confidence • Easy to order, install and use – Single Ethernet cable connection • Focused on improved productivity through automation • Updated premium performance hardware • Autosampler, Fraction Collector and Scale up Module for semi-prep • Quaternary pump, precision Column Heater, RI, Fluor, ELSD and autosampler for analytical • Updated Integrated Instrument Software Interface • Eliminate repetition, modernise UI • Consistent • Easy to use with added functionality • Adds to modular range 2

Varian 920 -LC Integrated Analytical HPLC 3

Varian 920 -LC What Is It? • Compact, stylish automated analytical HPLC – Pre-plumbed and factory tested for the highest confidence – Single cable communications • Virtually pulse-free dual piston pump – Does not require a pulse damper • State-of-the-art low pressure quaternary gradient module with built-in degasser • Autosampler with cooling option – Synchronised injection for outstanding retention time reproducibility – Compatible with 200 x 1. 6 ml HPLC vials or 3 microplates 4

Varian 920 -LC What Is It? • Either dual l UV-Vis or PDA detector – Optional second detector - Fluorescence or RI • High precision (<0. 15 o. C) dual column heater available – Low delay solvent pre-heater for improved heat transfer – Optional column switching and electric injection valve • NEW improved instrument interface on Galaxie. TM software • External CVM, ELSD or 356 -LC RI can be plugged directly into the back of the unit. 5

Varian 920 -LC Silver Bullets • Premium analytical pump performance and reliability • Superb Fast LC performance optimised for sub 3 um columns – Improved throughput without compromising robustness • • Unbeatable Injection performance and carryover statistics with 2008 loopless technology autosampler • Full system level computer controlled AIQ (validation) • 6 High sensitivity/top line performance detection systems across UV/PDA/RI/Fluor and ELSD – unparalleled strength Updated driver for enhanced user experience

920 -LC from Conventional to Fast HPLC 25 cm x 4. 6 mm Pursuit XRS 5 um 1. 0 ml/min 25 cm x 4. 6 mm Pursuit XRS 5 um 2. 2 ml/min 15 cm x 4. 6 mm Pursuit XRS 5 um 1. 0 ml/min 15 cm x 4. 6 mm Pursuit XRS 5 um 3. 75 ml/min 10 cm x 2. 0 mm Pursuit XRS 2. 8 um Ultra 0. 8 ml/min 5 cm x 2. 0 mm Pursuit XRS 2. 8 um Ultra 0. 4 ml/min 16 x Speed Enhancement 7 5 cm x 2. 0 mm 2. 4 um 1. 3 ml/min

Varian 920 -LC Fast LC PAHs Varian 920 -LC 6 minute Analysis Waters Acquity 7 Minute Analysis 8

Varian 920 -LC Solvent Delivery System • Dual piston quaternary pump produces fast gradients – even at 200 ml/min • Virtually pulse-free pumping without pulse damper • Outstanding gradient compositional accuracy give retention time precision at the <1% level • Built-in vacuum degas maintains pump prime and enhanced injection reliability • Overlay of 10 chromatograms • 10% steps at 1 ml/min 9 • Accurate flow from 0. 1 to 10 m. L/min for 2 to 4. 6 mm columns

Varian 920 -LC Autosampler • Needle-in-flow path geometry for low carry-over (<0. 01%) • Typical inj precision (< 0. 3% at 10 ml) • Optimized flow paths for fast injection turn-around • Synchronised injection for best RT reproducibility • Programmable wash setting for low carry-over • Injection volumes from 1 ul to 50 m. L • Cooled option available 10

Varian 920 -LC Washout Performance 125 ug/ml Caffeine Blank injection <0. 01% carryover 11

Varian 920 -LC Injection Synchronization 12

Sensitive UV-Vis Detectors Typical Agilent VWD S/N ~ 25 Typical Varian 920 -LC UV/Vis S/N > 36 13

Information Rich Diode Array Detection • 1024 pixels provides full coverage from 190 – 950 nm • Full l spectra collection • Enhanced purity • Automatic peak identification with customised libraries • Greater confidence on compound identification • Choice of flowcells • 4 mm path length semi-micro • 9 mm path length analytical • Front panel access • Lamps and flowcells • D 2 and Vis lamps self aligned 14 • Easily user maintained

Information Rich Diode Array Detection • Automatic peak identification with customised libraries • Use spectra as well as RT for compound ID • Greater confidence on compound identification • Peak search of unknown yields 998. 7 match for naphthalene 15

Varian 920 -LC Fluorescence Detector • Sensitive Fluorescence detector (S/N > 600 Raman H 2 O peak baseline method) • Time programmable l for increase sensitivity • Automatic l validation with built-in Hg lamp • Low 12 ml dead volume flowcell for improved chromatography 16

Varian 920 -LC Refractive Index Detector • Based upon 356 -LC RI detector • Low volume, flow efficient cell for superior peak shape • Stable, long life and high intensity LED • Minimises drift • Provides excellent detection limits • Enhanced life-time • Temperature controlled optics minimises baseline drift and provides outstanding detection limits • Complete automation thru Galaxie software 17

Varian 380 -LC and 385 -LC ELSD • Universal HPLC detector • Compatible with gradients • More sensitive than RI • NEW Glass Nebuliser for Improved Reproducibility • 2 to 3 Times More Sensitive • 40 Hz Data Rate for Fast LC • Complete Galaxie compatibility • Program changes thru a run 18

Heated Column Compartment • Temperature range from ambient + 10 o. C up to 65 o. C • Excellent temperature precision +0. 15 o. C – Ensures retention time reproducibility – Eliminates re-runs • Good temperature accuracy <1 o. C – Low delay volume pre-heater for efficient heat transfer to mobile phase • Rapid warm-up – Typically from ambient to 40 oc in <15 minutes • Spacious - easily accommodates 2 x 250 mm columns and 2 valves 19 • Option with electrically actuated column switching valve and injection valve

Excellent Long term Stability 20

Quick Warmup - Outstanding Stability 21

Reproducibility of 50 Injections Reproducibility <0. 8% over 50 injections and 12 hours 22

Varian 920 -LC Software Usability • User friendly Galaxie. TM software • Simplified, modern, consistent Instrument interface • Modular 11 pages • 920 -LC 4 pages!! • One page real-time status and chromatogram • Advanced integration and calibration • New IMS capabilities • Automated instrument validation • Reduces downtime and operating costs 23 • Fusion AE

Varian 920 -LC Integrated HPLC Special features of the Varian 920 -LC • Leak detector • Increases confidence with unattended automated runs • Single waste point • Ensures capture of all wastes • Easy to connect to waste vessel • Built-in Solvent bottle storage • Improved safety and convenience • Lower hight than modular systems 24

Varian 920 -LC Integrated HPLC Special features of the Varian 920 -LC • Clear front doors • Stylish • Provides access to autosampler without opening • Safety increased as lab staff shielded from solvent spray and leaks • Useful, convenient storage compartments • Column rack in door • Tool drawer • Operation log drawer 25

Varian 920 -LC AIQ (Validation) AIQ (Analytical Instrument Qualification) • Fully PC controlled IQ and OQ (not PQ) • Puts the Varian 920 -LC at the forefront of regulated capabilities • Consistent, reproducible methodology • Includes pumps, autosampler, detectors, column heater • Chargeable option • Fully documented 26

900 LC -Series AIQ • Full system test designed and implemented as part of the 900 -LC Series Analytical Instrument Qualification package. • Verifies the entire LC system by systematically running the following • • Wavelength Detector Accuracy Test • Auto-sampler Injector Accuracy Test (also measures Detector Linearity) • Auto-sampler Injector Precision Test • Auto-sampler Injector Carryover Test • Proportioning Accuracy Test • Detector Noise Test • Detector Drift Test • Auto-sampler Vial Identification Accuracy Test • Proportioning Linearity • 27 Flow Rate Accuracy Test Compositional Ripple

Varian 920 -LC Configurations pump inject detector Oven Detector 2 option Options Isocratic 00 -100890 -00 Dual piston pump 10 ml head Auto Dual l UV/Vis Opt FL, RI ELSD Gradient 00 -100891 -00 Quat, Grad, Degas Auto Dual l UV/Vis Opt FL, RI ELSD opt High Performance Gradient 00 -100892 -00 Quat, Grad, Degas Auto cooled Dual l UV/Vis Opt FL, RI ELSD opt Development 00 -100893 -00 Quat, Grad, Degas Auto PDA Std FL, RI ELSD opt Research 00 -100894 -00 Quat, Grad, Degas Auto cooled PDA Std FL, RI ELSD opt 28

Varian 920 -LC Consumables • Excellent range of HPLC Columns providing industry leading resolution and robustness • Pursuit XRS • Polymeric Phases - PL • From 2 mm to 2” ID Prepacked • Other Speciality Phases • NEW 2. 8 Ultra Column AND 2. 4 um Columns for Fast or High Res LC • 2. 8 um Ultra is optimised for Speed • 2. 4 um gives further enhancements in Resolution 29

2. 8 um Vs 2. 4 um – High Res or Extra Speed 2. 4 um 3 mm x 5 cm for RESOLUTION 2. 8 um 2 mm x 5 cm for 30 speed

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