Скачать презентацию Vaccines and Vaccine Delivery Systems of the Future Скачать презентацию Vaccines and Vaccine Delivery Systems of the Future

b28aee24fb3844f96195c069c832c109.ppt

  • Количество слайдов: 77

Vaccines and Vaccine Delivery Systems of the Future Bruce G. Weniger, MD, MPH Assistant Vaccines and Vaccine Delivery Systems of the Future Bruce G. Weniger, MD, MPH Assistant Chief for Vaccine Development Immunization Safety Branch Epidemiology and Surveillance Division National Immunization Program, CDC Course: Vaccines and Immunization - 2004 (IH 591 B / EPI 591 V) Rollins School of Public Health, Emory University, Atlanta, 19 November 2004

Vaccines and Vaccine Delivery Systems of the Future 1. Rapidly expanding basic science knowledge Vaccines and Vaccine Delivery Systems of the Future 1. Rapidly expanding basic science knowledge 2. Fruits of biotechnology: new and improved vaccines 3. Future vaccine constructs and technologies 4. Future (and re-emerging) vaccine delivery technologies

Journal Citations in National Library of Medicine* to Search Terms “Vaccines” or “Vaccination”, 1951 Journal Citations in National Library of Medicine* to Search Terms “Vaccines” or “Vaccination”, 1951 - 2002 * NLM MEDLINE Gateway: http: //gateway. nlm. nih. gov/gw/Cmd 2003 -Dec-02

NIH Jordan Reports on the Vaccine Development Pipeline NIH Jordan Reports on the Vaccine Development Pipeline

Maurice Hilleman ! ! ! Walter Reed Army Institute of Research Merck and Company Maurice Hilleman ! ! ! Walter Reed Army Institute of Research Merck and Company Shepherded many cell culture products from the laboratory to commercial availability, including:

2. The Fruits of Biotechnology: New and Improved Vaccines ! ! Newly Vaccine-Preventable Diseases 2. The Fruits of Biotechnology: New and Improved Vaccines ! ! Newly Vaccine-Preventable Diseases Recent/Expected Improvements on Existing Vaccines

Newly Vaccine-Preventable Diseases Newly Vaccine-Preventable Diseases

Recent/Expected Improvements on Existing Vaccines U. S. Licensed ! Hepatitis B, recombinant 1986 ! Recent/Expected Improvements on Existing Vaccines U. S. Licensed ! Hepatitis B, recombinant 1986 ! Haemophilus influenzae B, conjugated 1990 for use in infants ! Polio, inactivated, enhanced potency 1990 ! Pertussis, acellular 1992 ! Streptococcus pneumoniae , conjugated 2000 for use in infants

3. Future Vaccine Constructs and Technologies ! Genetically-engineered live vectors ! Nucleic acid vaccines 3. Future Vaccine Constructs and Technologies ! Genetically-engineered live vectors ! Nucleic acid vaccines ! Recombinant plants and plant viruses ! Synthetic immune-stimulating complexes ! Microencapsulation of antigen ! New adjuvants

Traditional Vaccine Type Classifications - I Traditional Vaccine Type Classifications - I

Traditional Vaccine Type Classifications - II Traditional Vaccine Type Classifications - II

Newer Vaccine Type Classification(s) Newer Vaccine Type Classification(s)

Future Vaccine Constructs and Technologies – Recombinant Live Vectors Future Vaccine Constructs and Technologies – Recombinant Live Vectors

Future Vaccine Constructs and Technologies – Recombinant Live Vectors Future Vaccine Constructs and Technologies – Recombinant Live Vectors

Future Vaccine Constructs and Technologies – Nucleic Acid Vaccines Future Vaccine Constructs and Technologies – Nucleic Acid Vaccines

Bioengineered Plant Vaccines - 1 Bioengineered Plant Vaccines - 1

Bioengineered Plant Vaccines - 2 Source: Charles Arntzen, Arizona State Univ. , www. hhs. Bioengineered Plant Vaccines - 2 Source: Charles Arntzen, Arizona State Univ. , www. hhs. gov/nvpo/meetings/dec 2003/Contents/Friday. AM

Bioengineered Plant Vaccines - 3 Source: Charles Arntzen, Arizona State University Bioengineered Plant Vaccines - 3 Source: Charles Arntzen, Arizona State University

Bioengineered Plant Vaccines - 4 Bioengineered Plant Vaccines - 4

Synthetic Virus-like Particles as Vaccine Constructs ! ! “Virosomes™” ! “Phage T 4 Display” Synthetic Virus-like Particles as Vaccine Constructs ! ! “Virosomes™” ! “Phage T 4 Display” Cath U. Am. ) “ISCOMS®” (Berna Biotech) (CSL, Pty) “Proteosomes™” (ID Biomedical) “Alphavirus-like particles / VEE replicons” (Alpha. Vax) (Walter Reed AIR,

Synthetic Virus-like Particles - 1 Synthetic Virus-like Particles - 1

Synthetic Virus-like Particles - 2 Synthetic Virus-like Particles - 2

Future Vaccine Constructs and Technologies – Microencapsulation Future Vaccine Constructs and Technologies – Microencapsulation

“Cochleate” Encapsulation “Cochleate” Encapsulation

Future Vaccine Constructs and Technologies – Adjuvants Future Vaccine Constructs and Technologies – Adjuvants

4. Future (and Re-emerging) Vaccine Delivery Technologies 4. Future (and Re-emerging) Vaccine Delivery Technologies

Why Needle-syringe Alternatives Are Needed - 1 Why Needle-syringe Alternatives Are Needed - 1

Why Needle-syringe Alternatives Are Needed - 2 Why Needle-syringe Alternatives Are Needed - 2

Smallpox mass campaign, New York City, 1947 ! ! ! 12 cases (2 fatal) Smallpox mass campaign, New York City, 1947 ! ! ! 12 cases (2 fatal) of smallpox >5 million persons vaccinated in 1 -2 weeks Required “ 1, 000 s” of MDs, RNs

Polio Vaccine (POL IPV) Mass Campaigns, 1950 s Polio Vaccine (POL IPV) Mass Campaigns, 1950 s

Vaccine Doses Distributed in the U. S. , 1962 -2000 Millions of Doses, Net Vaccine Doses Distributed in the U. S. , 1962 -2000 Millions of Doses, Net Swine “flu” campaign MEA MUM RUB MEA-RUB MUM-RUB

Jet injectors for Mass Campaigns Jet injectors for Mass Campaigns

Immunogenicity of Jet-injected Vaccines www. cdc. gov/nip/dev/jetinject. htm#bibliography Immunogenicity of Jet-injected Vaccines www. cdc. gov/nip/dev/jetinject. htm#bibliography

Safety Concerns over Multi-use-nozzle Jet Injectors Safety Concerns over Multi-use-nozzle Jet Injectors

Archives of Internal Medicine 1990; 150: 1923 -1927 Archives of Internal Medicine 1990; 150: 1923 -1927

U. S. -licensed devices filled by end-user “off-tool” single- or multi-dose vial: Biojector® 2000 U. S. -licensed devices filled by end-user “off-tool” single- or multi-dose vial: Biojector® 2000 Antares Pharma Vitajet® Injex® Entire device 1 -use disposable: J-Tip® Some investigational devices intended for manufacturer pre-filling: Entire device 1 -use disposable (high speed? ): Mini-Imojet® Intra. Ject® Pen. Jet™

Biojector® 2000 Customer Location Applications No. Injections Oct. 2003 through Oct. 2004 Naval Hospital Biojector® 2000 Customer Location Applications No. Injections Oct. 2003 through Oct. 2004 Naval Hospital Great Lakes, USS Red Rover Great Lakes, IL Mass immunization, Healthcare operations for military recruits Over 350, 000 US Coast Guard Training Center Cape May, NJ Mass immunization, Healthcare operations for military recruits 35, 000 Naval Hospital and associated outpatient medical clinics Pensacola, FL Immunization for adult and pediatric patients 50, 000 Naval Ambulatory Care Clinic Port Hueneme, CA Immunization for adult patients, Mass immunization for service members Naval Hospital Bremerton, WA Immunization for adult and pediatric patients Naval Hospital Guam, Pacific Islands, USA Immunization for adult and pediatric patients, Mass immunization for service members 20, 000

JI-SC JI-IM NS-IM JI-SC: JI-IM: NS-IM: Source: Jackson, et al. Vaccine 2001; 19: 4703 JI-SC JI-IM NS-IM JI-SC: JI-IM: NS-IM: Source: Jackson, et al. Vaccine 2001; 19: 4703 -4709

Lectra. Jet® – Motorized Model Lectra. Jet® – Motorized Model

Lectra. Jet ® Cartridges Lectra. Jet ® Cartridges

Demonstration of Lectra. Jet® Jet Injector, High-speed Model Demonstration of Lectra. Jet® Jet Injector, High-speed Model

Convenient Intradermal Injection by Jet Injection Older MUNJI models ID nozzle IM nozzle Convenient Intradermal Injection by Jet Injection Older MUNJI models ID nozzle IM nozzle

Convenient Intradermal Injection by Jet Injection Newer DCJI models Convenient Intradermal Injection by Jet Injection Newer DCJI models

Future (and Re-emerging) Vaccine Delivery Technologies Future (and Re-emerging) Vaccine Delivery Technologies

Live, trivalent, attenuated influenza vaccine Live, trivalent, attenuated influenza vaccine

Virosome intranasal influenza vaccine Virosome intranasal influenza vaccine

Internal anatomy of nose Internal anatomy of nose

Intranasal Delivery of “Proteosomes™” ! ! Nanoparticles from bacterial outer membrane protein ID Biomedical, Intranasal Delivery of “Proteosomes™” ! ! Nanoparticles from bacterial outer membrane protein ID Biomedical, Canada Source: Louis Fries, ID Biomedical Corp. ; www. hhs. gov/nvpo/meetings/dec 2003/Contents/Friday. PM/

Source: Louis Fries, ID Biomedical Corp. ; www. hhs. gov/nvpo/meetings/dec 2003/Contents/Friday. PM/ Source: Louis Fries, ID Biomedical Corp. ; www. hhs. gov/nvpo/meetings/dec 2003/Contents/Friday. PM/

Source: Louis Fries, ID Biomedical Corp. ; www. hhs. gov/nvpo/meetings/dec 2003/Contents/Friday. PM/ Source: Louis Fries, ID Biomedical Corp. ; www. hhs. gov/nvpo/meetings/dec 2003/Contents/Friday. PM/

Optinose “Bi-directional” Nasal Delivery Device - Video http: //www. optinose. no/technology/optinose_bidirectionalvideo. asp Optinose “Bi-directional” Nasal Delivery Device - Video http: //www. optinose. no/technology/optinose_bidirectionalvideo. asp

Future (and Re-emerging) Vaccine Delivery Technologies Future (and Re-emerging) Vaccine Delivery Technologies

Aerosol measles vaccination Bulletin W HO ©October 2002 Infectious Diseases in Children ©May 2000 Aerosol measles vaccination Bulletin W HO ©October 2002 Infectious Diseases in Children ©May 2000

A New Device for Measles Mass Vaccination by Aerosol Low Thermal/Mechanical Stress on Virus A New Device for Measles Mass Vaccination by Aerosol Low Thermal/Mechanical Stress on Virus Compatible with Nasal Prong or Mask

Aerosol inhalation measles vaccination Aerosol inhalation measles vaccination

Future (and Re-emerging) Vaccine Delivery Technologies Future (and Re-emerging) Vaccine Delivery Technologies

Cutaneous Immunization - I Cutaneous Immunization - I

Source: Powder. Ject plc Source: Powder. Ject plc

Cutaneous Immunization - II Cutaneous Immunization - II

“Epidermal Powder Immunization” - 1 “Epidermal Powder Immunization” - 1

“Epidermal Powder Immunization” - 2 Human trial: trivalent inactivated influenza vaccine “Epidermal Powder Immunization” - 2 Human trial: trivalent inactivated influenza vaccine

On. Vax™ Microenhancer Arrays, Becton-Dickinson Mikszta, et al. Nature Medicine April 2002; 8(4): 415 On. Vax™ Microenhancer Arrays, Becton-Dickinson Mikszta, et al. Nature Medicine April 2002; 8(4): 415 -19

Other pathways through stratum corneum ! ! 3 M Corp. - “Microstructured Transdermal Systems” Other pathways through stratum corneum ! ! 3 M Corp. - “Microstructured Transdermal Systems” Alza Corp. ! Altea Therapeutics Macroflux® “Electroporation” via Pass. Port™ ! GA Tech - “microneedles” ! Many, many others

Laser “Ablation” of Stratum Corneum Norwood Abbey, Ltd. Victoria, Australia www. norwoodabbey. com Laser “Ablation” of Stratum Corneum Norwood Abbey, Ltd. Victoria, Australia www. norwoodabbey. com

Cutaneous Immunization - III Cutaneous Immunization - III

Iomai, Inc. , Gaithersburg, MD, USA Iomai, Inc. , Gaithersburg, MD, USA

Source: Iomai, Inc. Source: Iomai, Inc.

Cutaneous Immunization - IV Cutaneous Immunization - IV

Future (and Re-emerging) Vaccine Delivery Technologies Future (and Re-emerging) Vaccine Delivery Technologies

Aerosol Anthrax and Plague Vaccines Source: Jim Eyles, Dstl, MOD, www. hhs. gov/nvpo/meetings/dec 2003/Contents/ Aerosol Anthrax and Plague Vaccines Source: Jim Eyles, Dstl, MOD, www. hhs. gov/nvpo/meetings/dec 2003/Contents/

Spiros® Powder delivery system Dura Pharmaceuticals, Inc. San Diego, CA (Elan) Spiros® Powder delivery system Dura Pharmaceuticals, Inc. San Diego, CA (Elan)

Future (and Re-emerging) Vaccine Delivery Technologies Future (and Re-emerging) Vaccine Delivery Technologies

Oral vaccines Oral vaccines

Live Oral Cholera Vaccine Source: Myron Levine, University of Maryland Live Oral Cholera Vaccine Source: Myron Levine, University of Maryland

Live Oral Typhoid Vaccine Source: Myron Levine, University of Maryland Live Oral Typhoid Vaccine Source: Myron Levine, University of Maryland

Future (and Re-emerging) Vaccine Delivery Technologies Thank you. Future (and Re-emerging) Vaccine Delivery Technologies Thank you.