The Proteome. Works System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.
The Proteome. Works System ~ New Mass Spectrometry Systems ~ At Pittcon 2001 Micromass introduced a range TM of e-MS enabled MALDI-TOF MS / MS-MS & ESI LC-MS-MS systems and Mass. Informatics. TM Software to leverage TM The Proteome. Works System TM • M@LDI HT TM • Q-Tof Ultima TM • Q-Tof micro
![M@LDI HT TM [new instrument] Micromass’ new networked MALDI TOF-MS & sample management system](https://present5.com/presentation/320069a951359eb41944b3af4034547c/image-4.jpg "M@LDI HT TM [new instrument] Micromass’ new networked MALDI TOF-MS & sample management system")
M@LDI HT TM [new instrument] Micromass’ new networked MALDI TOF-MS & sample management system “ 2 D-gel-MS analyser for proteomics factories”
M@LDI HT TM - Networked MALDI-TOF MS REFLECTRON Peptide Mass Fingerprinting LINEAR Intact Proteins
M@LDI HT TM - Sample Management System 80 x 96/384 -Sample Targets (bar coded) VERTICAL AXIS MOTOR VERTICAL FLIGHT TUBE AND REFLECTRON HOUSING HORIZONTAL AXIS MOTOR X - Y ROBOTIC ARM ELECTRO MAGNETIC GRIPPER TARGET PLATE CASSETTE 2 IN COLUMN OUT COLUMN TARGET PLATE CASSETTE 1 BAR CODE SCANNER VACUUM LOCK LID
M@LDI HT TM - Networked MALDI-TOF MS • • Linear and reflectron ion optics options 80 x 96/384 -Sample target management system Bar coded sample target plates Automated Laser Positioning System (ALPS) for sample target alignment and sample indexing • Intelligent acquisition / calibration algorithm • Web-enabled Client-Server Mass-Informatics for interrogation of non-redundant protein d. B …converts samples into knowledge not ‘just’ spectra
Protein. Lynx Global SERVER TM web-enabled Massinformatics
Protein. Lynx Global SERVER export to PDQuest Annotation Tools TM
PDQuest Annotation Tools CLICK
MS & MS-MS Techniques for Peptide / Protein Analysis Technique Data Type Dynamic Range Specificity Speed Data Interpretation Constraints MH + Low High Known Protein d. B Low Medium High MS MALDI PSD MH + MS-MS MALDI MH + High Known Protein d. B Known nucleotide d. B High Medium Homologues (BLAST) Time (PCR) Fragments ESI + HPLCMH ++ & 2 Fragments *ESI + HPLCAmino Acid (de novo) Sequence
![Q-Tof Ultima family instruments] TM DDA [new TM Micromass’ Super. Premi um family of](https://present5.com/presentation/320069a951359eb41944b3af4034547c/image-12.jpg "Q-Tof Ultima family instruments] TM DDA [new TM Micromass’ Super. Premi um family of")
Q-Tof Ultima family instruments] TM DDA [new TM Micromass’ Super. Premi um family of MALDI MSMS and ESI MS-MS systems for PROTEOMI CS and structural analysis
Q-Tof Ultima …the Super. Premium family of MS-MS • Q-Tof Ultima MALDI • The high throughput MALDI MS-MS • Q-Tof Ultima API • The Super. Premium API LC-MS-MS • Q-Tof Ultima GLOBAL • The flexible research platform for API LC-MS-MS / MALDI MS-MS DDA TM
![Q-Tof Ultima MALDI [new instrument] TM • • Dedicated MALDI MS-MS system V &](https://present5.com/presentation/320069a951359eb41944b3af4034547c/image-14.jpg "Q-Tof Ultima MALDI [new instrument] TM • • Dedicated MALDI MS-MS system V &")
Q-Tof Ultima MALDI [new instrument] TM • • Dedicated MALDI MS-MS system V & W-OPTICS options Protein. Lynx Global SERVER e-MS enabled
Q-Tof Ultima MALDI with VOPTICS 10, 000 FWHM
Q-Tof Ultima MALDI with WOPTICS 20, 000 FWHM
MALDI MS-MS is superior to PSD ADH 1 pm/µl Q-Tof Ultima MALDI MS-MS
TM Protein. Lynx Global SERVER. . . Matching MS-MS spectra to d. B of known protein or nucleotide sequence.
![Q-Tof Ultima API TM [new instrument] • Dedicated API (ESI) LC-MS-MS system • V](https://present5.com/presentation/320069a951359eb41944b3af4034547c/image-19.jpg "Q-Tof Ultima API TM [new instrument] • Dedicated API (ESI) LC-MS-MS system • V")
Q-Tof Ultima API TM [new instrument] • Dedicated API (ESI) LC-MS-MS system • V & W-OPTICS options • DDA - Precursor Ion Discovery (patent applied for) – “Neutral Loss” – “Precursor Ion” – High resolution – Exact mass – Compatible with HPLC • Protein. Lynx Global SERVER • e-MS enabled
Q-Tof Ultima API with WOPTICS 20, 000 FWHM
Q-Tof Ultima API. . . comparison of V & W-OPTICS Resolution TM
The superior peptide sequence coverage provided by ESI MS-MS data enables de novo sequencing
Sequencing results from MS-MS of m/z 841. 5
![Q-Tof Ultima GLOBAL [new instrument] TM • Flexible API & MALDI MS-MS research platform](https://present5.com/presentation/320069a951359eb41944b3af4034547c/image-24.jpg "Q-Tof Ultima GLOBAL [new instrument] TM • Flexible API & MALDI MS-MS research platform")
Q-Tof Ultima GLOBAL [new instrument] TM • Flexible API & MALDI MS-MS research platform • API / MALDI changeover without venting • V & W-OPTICS options • LC-DDA-MS – Precursor ion discovery – “Neutral Loss” – “Precursor Ion” – Exact mass • Protein. Lynx Global SERVER
Q-Tof Ultima GLOBAL
![Q-Tof micro TM [new instrument] Micromass’ finest API Q-Tof technology for structural analysis &](https://present5.com/presentation/320069a951359eb41944b3af4034547c/image-26.jpg "Q-Tof micro TM [new instrument] Micromass’ finest API Q-Tof technology for structural analysis &")
Q-Tof micro TM [new instrument] Micromass’ finest API Q-Tof technology for structural analysis & protein characterisation… • SMALLER • SMARTER • WIRED DDA TM
Q-Tof micro …SMALLER ESI inlet technology ZSPRAY nanoflow TM • • • TM 5000 FWHM resolution 5 ppm RMS exact mass measurement TM Dynamic External Calibration (DXC ) 20000 m/z range
Q-Tof micro TM …SMARTER …WIRED • Data Directed Analysis (DDA) – Automates analysis of protein digests by LCDDA-MS • Precursor Ion Discovery (patent applied for) – “Neutral Loss” – “Precursor Ion” – High resolution – Exact mass • Compatible with HPLC • Maximising data quality
Data Directed Analysis DDA TM Exclusion of 1+ background ions
The New e-MS Enabled Mass Analysers of The TM Proteome. Works System. . . TM msinnovation. com
The Proteome. Works System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.
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