The Effect of Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries on the supression of Amaloid B Protein in C. elegans Roger Coleman
Need Chart 1 shows statistics and future predictions of Alzheimer's disease http: //www. alzalliance. org/images/Forcast%20 of %20 Alzheimer%27 s%20 disease%20 chart. gif
Knowledge Base Figure 1 shows C. elegans http: //shirleywho. files. wordpress. com /2008/09/c-elegans_esa. jpg
Knowledge Base Figure 2 shows the lifecycle of c. elegans
Knowledge Base Picture 3 shoes a cross reference of a normal brain and a Alzheimer's infected brain. http: //www. realage. com/health_guides/Alzheimer/i mg%5 CLiving. With. Alzheimers_artv 2. jpg
Knowledge Base Figure 4 shows an amyloid b protein http: //64. 202. 120. 86/upload/image/news/g etting-out-of-the-loop-of-alzheimer-sdisease/amyloid-protein-loop. jpg
Knowledge Base Chart 2 shows amaloid b protein product
Knowledge Base Figure 5 shows the plant of Ginkgo biloba http: //www. herbalextractok. com/Herbal. Extra ct/Ginkgo_Biloba_extract. jpg
Lit Review (Wu et, al, 2006)
Purpose To determine if ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries has an effect on Alzheimer dieses in C. elegans. Hypothesis Null hypothesis is that no effect will occur when ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries ware added. The Alternative hypothesis is that ginkgo biloba amyloid, freeze dried black raspberries and freeze dried blueberries
Methodology The Effect of Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries on the supression of Amaloid B in C. elegans C. Elegnas with amaloid b and ginkgo biblia solution (Experimental) C. Elegans fed with a 10 % solution of Ginkgo Biloba N=? C. Elegans fed with 10% solution of Freeze dried Black raspberries N=? C. Elegans fed with a 10 % solution of Freeze Dried Blueberries N= ? C. Elegnas with no solutions N= ? (Control) C. Elegans will be fed with 100 ul spots of e. coli (OP 5 O) on nematode growth medium and c. elegans will be cultured a 20 degrees C Results will be taken from visual analysis of C. elegans under a microscope.
Protocols for Measurement Grown on NGM and C. elegans will be fed with E. Coli (OP 5 O) with 100 ul spots. Amaloid B (AB) protein will be mixed with and without ginkgo biblia at 100 ug/ml solution( stord at 20 Degrees C) Ginkgo Biloba Amyloid, Freeze Dried Black Raspberries and Freeze Dried Blueberries will be given in the NGM in a 10 % solution.
Protocols for Measurement (cont) All solutions will be given after one day and analysis will be looked at after 2 days. Analysis will be compared ( under a microscope) to the number of normal and the number of paralyzed c. elegans
Do- ability C. elegans and chemicals are easy to buy Much of the equipment is already in the lab Chemicals are easily applied to C. elegans
Budget Vendor Item Number Cost per unit Total Cost Bulk Nutrition Ginkgo Biloba powder 8. 99 $8. 99 ANA SPEC [Gln 11] - beta 25369 - Amyloid (1 16) $90 Nutri. Fruit Freeze Dried Black Raspberry Powder 26 -b 8 wl-f 1 ku $25. 95 Nutri. Fruit Freeze Dried Blueberry Powder BT-4 AXLSRXH $20. 95
Budget (cont) Vendor Item Carolina Biological Item Number Unit Cost Total Cost Caenorhabditis 173500 elegans N 2, Living, Culture $10. 95 Carolina Biological Nematode Growth Agar 173520 $6. 25 (Quantity of 2) $12. 50 Carolina Biological Erwinia. Escherichia coli, living tube 155065 $9. 20 178. 54
Bibliography Works Cited Hancock, Robert D. "Berry fruit as ? superfood? : . " Scottish Crop Research Group 54 (2007). Http: //www. iob. org/userfiles/File/biologist_archive/Biol_54_2_Hancock. pdf. Luo, Yuan. "Inhibition of amyloid -b aggregation and caspase- 3 activation by the Ginkgo biloba. " Departments of Biological Sciences and Chemistry and Biochemistry (2002). Wilson, Mark A. "Blueberry polyphenols increase lifespan and thermotolerance in Caenorhabditis elegans. " Pub. Med Central (2006). Wilson, Mark. "Bluebrry polyphenols increase the lifespan of c. elegans. " Blackwell Publishing Ltd (2006). Wu. "Egb reduces ab specific pathological behaviors are suppressed by ginkgo biloba extract Egb 761 in caenorhabditis elegans. " (2006). Yang, Huan. "The cytokine activity of HMGB 1. " Laboratory of Biomedical Science.
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