The comparing of natural antibacterial substance on E

The comparing of natural antibacterial substance on E. Coli Manhasset Science Research Jean Cheng Period 4

Need Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil http: //www. scielo. br/img/revistas/bjm/v 39 n 4/arq 27_fig 01. jpg

Knowledge Base Figure 2: Chemical formula for Penicillin http: //itech. dickinson. edu/che mistry/wp content/uploads/2008/04/peni cillin core 1. png

Knowledge Base (cont) Peptidoglycan Figure 3: Penicillin attacking the peptidocglycan http: //upload. wikimedia. org/wikipedia/commons/thumb/8/8 b/Gram_negati ve_cell_wall. svg/800 px Gram_negative_cell_wall. svg. png

Knowledge Base (cont) Figure 4: Tea tree’s terpene chemical formula http: //www. wacker. com/cms/app. L/mdm_im ages/R 67_IV 1_ 432011277_8959. jpg Figure 5: Pectin Chemical formula http: //sci toys. com/ingredients/pectin_2. gif

Knowledge Base (cont) Figure 6: Aloe vera http: //apps. who. int/medicinedocs/fr/d/Js 2200 e/5. html

Knowledge Base (cont) Figure 7: Citral http: //www. impgc. com/images/Structures/Citral. bmp

Literature Review Figure 8: Chemical Change in Allicin subjected to different conditions. (Ariga, et al. 2008) http: //media 2. web. britannica. com/eb media/33/7433 004 96 DFD 197. gif

Literature Review (cont) Figure 9: Antimicrobial activity of aloe vera gel (Alemdar, et al. 2009)

Literature Review (cont) Figure 10: Yuzu fruit seed oil on different microbial (Nakazono, et al. 2006)

Literature Review (cont) Figure 11: 12 different essential oil on different species of bacteria. (Rusenova, et al. 2009)

Purpose l Find which natural substance is best in inhibiting E. coli. l Null Hypothesis: No effect l Alternative Hypothesis: Tea tree oil is the best antimicrobial substance against E. coli

Methodology The comparing of natural antibacterial substance on E. Coli Essential oil and antibiotic plant substance N=15 Aloe (anthraquinones) N=3 Garlic (allicin) N=3 Ginger (citral) N=3 Petri dish, E. coli, filter paper, glass spreader Disc diffusion assay method Millimeter SPSS ANOVA Test Tea Tree oil N=3 Yuzu fruit oil N=3

Protocols Paper disk with substance being tested Bacteria on agar Inhibited Zone(mm) Figure 12: Disk Diffusion method http: //diverge. hunter. cuny. edu/~weigang/Images/20 17_diskdiffusion. jpg

Protocols (cont) Figure 13: http: //gsbs. utmb. edu/microbook/imag es/fig 10_5. jpg

Do Ability l Not expensive l doesn’t require large amount of space l Some materials found in lab or supermarket l E. coli is needed as well as agar, aloe, tea tree, yuzu, and garlic l Natural antiseptic doesn’t have negative effect

Budget

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