State Veterinary Administration of the Czech Republic BSE

State Veterinary Administration of the Czech Republic BSE Testing in the Czech Republic MVDr. Josef Holejsovsky Chief Veterinary Officer Praque, 24. 10. 2002

Protective Measures related to BSE in the Czech Republic I Since 1962 Animal waste processed according to the Methodical Instruction of the Ministry of Agriculture, Forestry and Aquaculture issued to the Decree No. 154/1961 of the Act on Veterinary Care: Ø Ø sterilisation for the time period of 30 minutes, at the temperature of 130 -140 °C, at the pressure of 3 bars, further boiled until their disintegration.

Protective Measures related to BSE in the Czech Republic II 04. 06. 1991 The first official ban on feeding of MBM from ruminants and minks to ruminants was issued. 30. 09. 1996 The official ban from June 1991 has been repeatedly confirmed and extended on using of all mammalian animal tissues and all products thereof for feeding of all ruminant species.

Protective Measures related to BSE in the Czech Republic III 01. 2000 SRM is removed from food chain based on Decree No. 286/1999 Coll. 18. 06. 2001 The ban in force on production of mechanically recovered meat from all bones of cattle, sheep and goats of all age categories; SRM from slaughterhouses and dead animal cadavers is removed from feed chain, processed in one designated rendering plant for a following incineration.

Protective Measures related to BSE in the Czech Republic IV CR issued the ban on imports of bovine animals and products thereof: Great Britain and Switzerland 1994 France and Portugal 21. 11. 2000 Belgium, Denmark, Irish Republic, Luxembourg, the Netherlands, Germany, Spain 01. 12. 2000 Italy 18. 01. 2001 ***************** CR issued the ban on imports of MBM from all EU Member States 27. 11. 2000

Monitoring of BSE in the Czech Republic I 1991 The TSE Reference Laboratory has been established. Up to 1996 The number of tests was limited - only animals with clinical symptoms of nervous disease were examinated by means of classical histological technique.

Monitoring of BSE in the Czech Republic II 01. 02. 2001 Tests using the Western blot method (Prionics check) have been introduced, the risk groups of animals were tested in: Ø State Veterinary Institute Prague, Ø State Veterinary Institute Jihlava. Confirmation method: The immunocytochemistry started to be used in the State Veterinary Institute Jihlava and than in Prague. 12. 06. 2001 Cattle aged over 30 months have been and are being compulsorily tested.

Monitoring of BSE in the Czech Republic III January 2001 The Crisis Committee was established by the Minister of Agriculture of the Czech Republic in relation to BSE - representatives of: Ø Ministry of Agriculture, Ø State Veterinary Administration CR, Ø Ministry of Health, Ø Ministry of Foreign Affaires, Ø Ministry of Trade. Ministry of Health established the National Reference Laboratory for human TSE’s (CJD/V-CJD)

BSE Testing Data Flow in the Czech Republic DBF file monthly transfered Data aggregation XLS file weekly transfered State veterinary administration CR Headquarters Animal transport District veterinary administration State veterinary supervision Cadaver transport Final output Slaughterhouse Rendering plant Sample request State veterinary institute sampling Stable Result of laboratory testing

I Am BSE Free !!!

BSE Tests carried out using WB Method 01. 02. 2001 to 11. 06. 2001 Under 24 months From 24 to 30 months Over 30 months Total CZ origin Import Total killed and destroyed animals 0 0 0 suspected animals 0 0 0 fallen stock animals 3 1 4 15 3 18 458 105 563 476 109 585 emergency slaughtered animals 8 0 8 43 4 47 2034 261 2295 2085 265 2350 slaughtered animals 3575 0 3575 1819 0 1819 2998 280 3278 8392 280 8672 TOTAL 3586 1 3587 1877 7 1884 5490 646 6136 10953 654 11607 Finding No. Sex Age Origin Breeder Date of results CZ 000014541607 Female 68 month CZE ZD Dusejov 5. 6. 2001

Origin of Tested Cattle 01. 02. 2001 to 11. 06. 2001 19 DC MO TP UL RA PS AB BE RK PU BR SU OP CR SY BN PB JE HK KH PM DO NA KO PY PZ RO JC NB KL CH TC TU SM MB ME LN KV JN CL LT CV SO LB NJ HB PJ ZR PI KT JI ST TR BO JH PT PV BK PE TA OS KI VS KM VY BM FM PR ZL UH CB ZN HO BV CK from 1 to 100 from 301 to 500 from 1001 to 3000 from 101 to 300 from 501 to 1000 over 3000

Localization of the First BSE Finding Polna Farm Dusejov Jihlava Slaughterhouse Kostelec u Jihlavy CZ 100 Trest Telc

History of the First BSE Finding Cow Nr. CZ 000014541607 from the Farm Dusejov, district Jihlava 04. 06. 2001 slaughtered in export slaughterhouse „Kostelecke uzeniny“, Kostelec u Jihlavy, CZ 100 05. 06. 2001 State veterinary institute in Jihlava - positive Western Blot test 06. 2001 State veterinary institute Prague - positive second Western Blot test 08. 06. 2001 positive imunohistochemical test in the Czech laboratory 14. 06. 2001 confirmation in the European reference laboratory Tuebingen

Genealogy of the First BSE Finding Cow Nr. CZ 000014541607 from the farm Dusejov, district Jihlava born 28. 09. 1995, no moving, Czech origin, slaughtered 68 months old Mother: Cow Nr. CZ 000080965647, born 29. 06. 1993, still living in Dusejov Siblings: 1. Cow CZ 000014827607, born 16. 09. 1996, no moving, slaughtered 16. 04. 2001 in Kostelec, tested for BSE in SVI Jihlava with negative result 2. Cow CZ 000030163607, born 13. 01. 1998, still living in Dusejov 3. Bull CZ 000116554667, born 16. 01. 2000, still living in Dusejov 4. Heifer CZ 000115283607, born 05. 01. 2001, still living in Dusejov Offspring: 1. Cow CZ 000030150607, born 30. 11. 1997, still living in Dusejov 2. Heifer CZ 000115014607, born 01. 2000, death in the first month 3. Heifer CZ 000115271607, born 07. 12. 2000, still living in Dusejov

Cattle Killed and Destroyed in the Farm Dusejov total 134 animals !! BSE test without positive findings !!

Veterinary Measures in the Herd of Origin a) Movement restrictions b) Eradication by the methods „cohort cull and offspring cull“ c) Testing of all culled animals: - if positives - incinerated - if negatives - burried a) Other animals - movement restrictions with the exception of movement to slaughterhouse with veterinary certificate - all dead and slaughtered animals compulsory test by Western blot

Veterinary Measures in the Slaughterhouses a) All bovine animals over 30 months of age subject to slaughter have been compulsory testing for BSE since 12 th June 2001 b) Ban of production of mechanically recovered meat from bones of bovine animals since 18 th June 2001 c) Separate collection, removal and transport of SRM to designated rendering plant since 18 th June 2001

Collecting Areas of the Rendering Plants Mimon DC Podborany MO TP CL TU SM LT JC MB Mankovice NA ME LN KV AB CH PS BE PY PZ KH PM OP CR ZR PI KT TA JI ST TR BO JH PT PV BK PE OS NJ HB PJ DO Birkov BR SU SY BN PB RK PU KO RO JE HK NB KL RA TC Zichlinek JN UL CV SO LB FM PR VS KM VY BM KI ZL UH CB HO ZN BV CK Chotycany (SRM rendering) Vez Medlov Otrokovice

Veterinary Measures Concerning SRM a) Removal from food chain applied since 1 st January 2000 b) Removal from feed chain - separate processing of SRM from slaughterhouses and rendering plants in one designated rendering plant under veterinary supervision since 18 th June 2001

Extraordinary veterinary measures issued on 18. 6. 2001 (following the 1 st BSE case) v SRM is collected at slaughterhouses separately from the other condemned materials v Separated collection of SRM from dead animals at rendering plants v Ban on mechanical recovering of meat from the bones of cattle, sheep and goats v Transport of all SRM to the only one designated rendering plant v In addition to this measures issued by the Minister of Agriculture of the CR it was decided, in the frame of CVO responsibility, to test for BSE all cattle aged more than 30 months (or more than 24 months in emergency slaughtering)

Amendments of decrees related to the Veterinary Act in addition to the Extraordinary Veterinary Measures issued on 18. 6. 2001 v SRM staining after veterinary examination v burning or burrying of rendered SRM v keeping of records concerning SRM processing for next 7 year v using of native animal origin wastes only for animal feeding in ZOO gardens, circusses and farms of carnivorous fur bearing animals only under approval of DVA. Native and frozen waste materials coming from the place of origin directly to the user (without any intermediaries) v after stunning of cattle, sheep and goats ban on mechanical destruction (laceration) of brain tissue in the cerebral cavity

Measures to be taken following the accession of the Czech Republic to the EU v processing of dead animals by the same way as SRM (2001/25/EC) v ban on feeding of meat-and-bone meals for all domestic animals (2000/766/EC)

Did you hear it? She is BSE positive!

BSE Tests carried out using WB Method - 01. 02. -27. 08. 2001 Under 24 months From 24 to 30 months Over 30 months Total CZ origin Import Total killed and destroyed animals 3 0 0 0 135 138 0 138 suspected animals 1 0 1 2 0 2 10 1 11 13 1 14 fallen stock animals 4 2 6 20 3 23 926 151 1 077 950 156 1 106 emergency slaughtered animals 35 0 35 899 4 903 10 712 359 11 071 11 646 363 12 009 slaughtered animals 5 067 12 5 079 2 862 0 2 862 22 365 439 22 804 30 294 451 30 745 TOTAL 5 110 14 5 124 3 783 7 3 790 34 148 950 35 098 43 041 971 44 012 Finding No. Sex Age Origin Breeder Date of results CZ 000014541607 Female 68 month CZE ZD Dusejov 5. 6. 2001 CZ 000018136614 Female 72 month CZE SZ Beran 21. 8. 2001

Origin of Tested Cattle 01. 02. -27. 08. 2001 30 DC MO TP UL AB BE DO BR SU OP CR SY BN PB RK PU KH PM JE HK KO PY PZ RO NA NB RA PS JC MB KL CH TU SM ME LN KV TC JN CL LT CV SO LB NJ HB PJ ZR PI KT JI ST TR BO JH PT PV BK PE TA OS KI VS KM VY BM FM PR ZL UH CB ZN HO BV CK from 1 to 100 from 301 to 500 from 1001 to 3000 from 101 to 300 from 501 to 1000 over 3000

Localization of the Second BSE Finding Breeder Beran, Farm Svetnov Slaughterhouse Radostin nad Oslavou Zdar nad Sazavou Nove Mesto na Morave Bystrice nad Pernstynem Velke Mezirici Velka Bites

Localization of the both BSE Findings • ZD Dusejov • SZ Beran

BSE tests carried out by using of rapid tests 01. 02. 2001 to 31. 12. 2001 Under 24 months From 24 to 30 months Over 30 months Total CZ origin Import Total killed and destroyed animals 3 0 0 0 135 138 0 138 suspected animals 1 0 1 9 0 9 99 6 105 109 6 115 fallen stock animals 5 2 7 63 3 66 1 872 204 2 076 1 940 209 2 149 emergency slaughtered animals 48 0 48 3 375 6 3 381 25 400 514 25 914 28 823 520 29 343 slaughtered animals 6 577 12 6 589 4 215 9 4 224 70 817 771 71 588 81 609 792 82 401 TOTAL 6 634 14 6 648 7 662 18 7 680 98 292 1 495 99 818 112 619 1 527 114 146

Number of BSE tests in districts 01. 02. 2001 to 31. 12. 2001 DC MO TP UL RA PS AB BE RK PU BR SU OP CR SY BN PB JE HK KH PM DO NA KO PY PZ RO TC JC NB KL CH TU SM MB ME LN KV JN CL LT CV SO LB NJ HB PJ ZR PI KT JI ST TR BO JH PT PV BK PE TA OS KI VS KM VY BM FM PR ZL UH CB ZN HO BV CK from 1 do 100 from 301 to 500 from 1001 to 3000 from 101 to 300 from 501 to 1000 over 3000

BSE tests carried out by using of rapid tests 01. 02. 2001 to 30. 09. 2002 under 24 months CZ origin import from 24 to 30 months total CZ origin import over 30 months total CZ origin import total "A" 3 0 0 0 135 138 0 138 "B" 6 0 6 17 0 17 109 8 117 132 8 140 "C" 11 2 13 156 3 159 3 490 248 3 738 3 657 253 3 910 "D" 48 0 48 8 751 7 "E" 0 0 0 15 0 "F" 6 828 12 6 840 4 493 10 total 6 896 14 6 910 13 432 „A“ „B“ „C“ „D“ „E“ „F“ 8 758 54 163 15 35 699 54 862 62 962 0 35 50 706 63 668 0 50 4 503 164 086 2 401 166 487 175 407 2 423 177 830 20 13 452 222 018 3 356 225 374 242 346 3 390 245 736 killed and destroyed animals suspected animals fallen stock animals emergency slaughtered animals sick at slaughtered animals

Number of BSE tests in districts 01. 02. 2001 to 30. 09. 2002 LB DC MO TP UL MB KL PS TC DO AB BE PM PY PZ RO 5 762 6 387 KH CR ST ZR CB 6 298 6 953 OL BO 9 074 BM FM VS KM VY HO ZN NJ KI PR PV JI TR OS 5 240 BK 9 388 8 011 JH OP 6 507 PE 8 241 BR SU SY HB 5 241 PT UO 8 302 7 041 TA JE RK PU 5 832 PI KT HK KO BN PB 5 190 PJ NA NB RA CH TU JC ME LN KV SO SM LT CV JN CL ZL UH BV CK from 1 to 500 from 1 001 to 2 000 from 3 001 to 5 000 from 501 to 1 000 from 2 001 to 3 000 over 5 000

BSE tests carried out by using of rapid tests 01. 02. 2001 to 14. 10. 2002 „A“ „B“ „C“ „D“ „E“ „F“ killed and destroyed animals suspected animals fallen stock animals emergency slaughtered animals sick at slaughtered animals

Vyšetření BSE rychlými testy 01. 02. 2001 až 31. 01. 2005 do 24 měsíců ČR dovoz 24 až 30 měsíců celkem ČR dovoz nad 30 měsíců celkem ČR dovoz celkem "A" 18 0 18 4 0 4 1 992 0 1 992 2 014 0 2 014 "B" 6 0 6 20 0 20 114 8 122 140 8 148 "C" 146 2 148 9 168 6 9 174 65 960 323 66 283 75 274 331 75 605 "D" 49 0 49 21 949 8 "E" 1 0 1 75 0 "F" 6 888 12 6 900 4 770 10 4 780 467 870 3 904 471 774 479 528 3 926 483 454 celkem 7 108 14 7 122 35 986 24 36 010 667 539 5 119 672 658 710 633 5 157 715 790 21 957 131 377 75 883 132 260 153 375 226 1 „A“ utracená zvířata „B“ podezřelá zvířata „C“ uhynulá zvířata „D“ nutně poražená zvířata „E“ přeřazená zvířata „F“ zvířata z normálních porážek 227 302 891 154 266 1 303

BSE tests carried out by using of rapid tests 01. 02. 2001 to 31. 01. 2005 under 24 months CZ origin import from 24 to 30 months total CZ origin import over 30 months total CZ origin import total "A" 18 0 18 4 0 4 1 992 0 1 992 2 014 0 2 014 "B" 6 0 6 20 0 20 114 8 122 140 8 148 "C" 146 2 148 9 168 6 9 174 65 960 323 66 283 75 274 331 75 605 "D" 49 0 49 21 949 8 "E" 1 0 1 75 0 "F" 6 888 12 6 900 4 770 10 4 780 467 870 3 904 471 774 479 528 3 926 483 454 total 7 108 14 7 122 35 986 24 36 010 667 539 5 119 672 658 710 633 5 157 715 790 „A“ 21 957 131 377 75 226 883 132 260 153 375 1 killed and destroyed animals „B“ suspected animals „C“ fallen stock animals „D“ emergency slaughtered animals „E“ sick at slaughtered animals „F“ slaughtered animals 227 302 891 154 266 1 303

Number of BSE tests in districts 01. 02. 2001 to 31. 01. 2005 from 1 to 1 000 from 5 001 to 10 000 from 15 001 to 20 000 from 1 001 to 5 000 from 10 001 to 15 000 over 20 000

Původ vyšetřeného skotu na BSE 01. 02. 2001 až 31. 01. 2005 1 až 1 000 5 001 až 10 000 15 001 až 20 000 1 001 až 5 000 10 001 až 15 000 více než 20 000