Southern blot analysis of the X-linked red-green gene locus. (A) Partial restriction maps of the red and green pigment genes. E, Eco. Rl; B, Bam. Hl; R, Rsal. Open boxes denote the six exons. The restriction fragments A–D used in distinguishing red from green-specific sequences are shown below the genes. The A–C fragments derived from the red opsin gene (Ar, Br, Cr) are longer than those derived from the green gene (Ag, Bg, Cg) due to a 1. 5 -kb insertion in the first intron (wavy line). The absence of a Rsal site in the green opsin exon 5 accounts for the larger Rsal fragment of the green opsin gene (Dg). (Adapted with permission from Nathans et al. 11 ) (B) Autoradiograph of Southern blots of genomic DNA samples from males with normal and defective color vision digested with either a combination of Eco. Rl and Bam. Hl or Rsal. The Eco. Rl-Bam. Hl fragments (B and C) were detected by hybridization to a Source: Color Vision and Its Genetic Defects, The Online Metabolic and Molecular Bases of Inherited Disease 350 -bp c. DNA probe encompassing exon 1 and part of exon 2, whereas the D fragments were detected by a 400 -bp genomic probe from the 3′ end of Citation: Valle D, Beaudet AL, Vogelstein B, Kinzler KW, patterns were selected. A, Gibson individual G The Online Metabolic a single red. intron 4 of the green opsin gene. 11 Typical examples of Southern blot Antonarakis SE, Ballabio. Lane 1: An K, Mitchellwith protanopia who has and Molecular Bases the. Inherited D fragments of. Available at: http: //mhmedical. com/ Accessed: March 13, 2018 the green pigment gene. Lane 2: A of C and Disease; 2014 the red gene replaced by the corresponding fragments of green hybrid gene with Copyright © 2018 Mc. Graw-Hill Education. All rights reserved deuteranomalous individual who has normal red pigment gene and a green-red hybrid pigment gene (see diagram in part C). Lane 3: An individual with deuteranopia who has only a normal red gene (see diagram in part C). Lane 4: A deuteranomalous individual with a gene array comprised of normal red
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