Scanning Probe Microscope Prepared by Baktybaev Dauren Checked by Galiya Kadurhanovna
History of the Microscope • 1590 –first compound microscope
History of the Microscope • 1655 – Robert Hooke used a compound microscope to observe pores in cork –He called them “cells”
History of the Microscope • Antoine van Leeuwenhoek st to see single-celled – 1 organisms in pond water
Types of Microscopes • 1. Compound Light Microscope – 1 st type of microscope, most widely used –light passes through 2 lenses –Can magnify up to 2000 x
Ocular lens Objective lenses
Types of Microscopes • 2. Electron Microscope –Used to observe VERY small objects: viruses, DNA, parts of cells –Uses beams of electrons rather than light –Much more powerful
Scanning Probe Microscope • It is class of microscopes which uses to getting picture of surface or of its local characteristics. • SPM was created in 1981 by Carl Binning and Enrich Rorer, and later in 1986 was reward by Nobel Prize in physics
The principle of SPM working • Process of picture forming it is multistep process, and based on the interaction between samples surface and probe. For registration use many types of sensors, which have a different sensitivity.
• 1 st step it is the selecting of probe • 2 nd step it is scanning of sample by certain probe • 3 rd it is providing of data to computer • 4 th step it is the building of math formula • 5 th step it is the decoding of math formula, and building of picture in 3 D format
The main problems of SPM • 1) Selecting of probes, every probe has own sensitivity, radius, and each probes have own detection area(f. e. : some probes detect only viruses, other microbes and others) • 2) Little scanning area(S=150 mkm) • 3)Duration of process
Modern state of SPM • Nowadays SPM use in complex sciences such as biochemistry, genetics, biophysics, molecular biology and others.
Thank you for your attention!!!
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