Saint-Petersburg State University Institute of Earth Science Non-pollen palynomorph: green algae Bas Van Geel, Institute for Biodiversity and Ecosystem Dynamics, The Netherlands Kondakova Veronika, 1 st year master student
Pollen preparation method • • • Boil the sample in 10% KOH Sieve Wash the material Dehydrate with acetic acid Acetolyse the material by heating the sample in the acetolysis mixture to 100 °C. Acetolysis dissolves most of the tissue and organic debris, and removes the proteins, lipids, and carbohydrates from the surface of the pollen grains. This makes the pollen grains easier to stain, photograph, and identify. Cool the sample tubes, wash with distilled water alcohol. The separation of organic material from sand clay Put the residue tube for a night in the oven at 40 °C. Prepare microscope slides.
Zygnemataceae • inhabiting shallow, stagnant, oxygen-rich freshwater lakes, ponds, small pools or wet soils. • reproduce during spring in shallow, relatively warm water. • for identification to species level in most cases the morphological characteristics of the spores are necessary. • range from the Carbon to the present time.
Pediastrum • • • radially-symmetrical colonial green algae. The cells show one or two horns. Records of Pediastrum seem to indicate a wide range of environmental responses. Factors which causative for the occurrence of Pediastrum in sediments include changes in erosion, turbidity, water chemistry, nutrient status, and p. H. Pediastrum species are common in hard-water eutrophic lakes.
Botryococcus • colonial green algae with densely-packed conical cells radiating and branching from the center. • Modern Botryococcus is widely dispersed in temperate and tropical regions, and is known to tolerate seasonally cold climates. • lives in freshwater fens, temporary pools, ponds and lakes,
• • • Cyanobacteria The analysis allows a better understanding of the changing local environments in lakes and pools in the past. a pioneer role in nutrient poor conditions, thanks to its ability to fix nitrogen and thus making conditions suitable for other aquatic plants. Phoyosynthesis – process characteristic of cyanobacteria, the latter not exhibited by any other procaryotrs
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Non-pollen palynomorph.pptx