® R. A. P. I. D. LT Food Security System Dennis D’Alfonso National Sales Manager
Idaho Technology (ITI) – Company Profile Idaho Falls, ID (1990 -1999) Salt Lake City, UT (1999 -PRESENT) 200+ Employees: – Hardware, Firmware, Software – – R&D Molecular Biology, Chemistry IT Biochemistry and Freeze Drying Production & Assembly Sales, Marketing, and Customer Service 3 Facilities – Salt Lake City - Research and Development - Software Engineering - Chemistry Production
Proven Technology IN and OUT of Lab 1991 Rapid. Cycler R. A. P. I. D. ® System is a rugged, portable BW detection system for the field and Lab. Freeze dried reagents for Pathogen detection. 1996 Light. Cycler 1999 R. A. P. I. D. & Reagents 2002 RAZOR 2006 R. A. P. I. D. LT RAZOR® System is a rugged, portable and battery powered BW detection system for the front line soldier and first responder. The R. A. P. I. D. ® LT is a real-time PCR instrument capable of analyzing samples for the presence of targeted nucleic acid sequences.
ITI Customers • US Government – – Do. D DHS USDA EPA • US Commercial – Fortune 200 companies • International – Europe • • • Italian Government Czech Republic UK Switzerland Finland – Australasia • Australia • Singapore • Japan
R. A. P. I. D. ® LT Food Security System Benefits: • • Sensitive Specific Very fast PCR (in less than an hour!) Designed for minimally trained users – Freeze-dried reagents – Simple set-up – Point and click automated software • Pooling enabled – Expands testing by 5 X – Reduces cost in a negative world • Simultaneous testing of all targets
Daily Capacity 55 minute run Single Unit Capacity 8 hr day – 256 reactions 16 hr – 512 reactions 24 hr – 768 reactions Pooling Capacity 8 hr day – 1280 reactions 16 hr day – 2560 reactions 24 hr day – 3840 reactions
R. A. P. I. D. LT Workflow Continues to Step 3 & 4 >>
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R. A. P. I. D. LT Workflow
Enrichment and Preparation Enrichment Times Target Time Salmonella 16 hours E. coli O 157: H 7 8 hours Listeria spp. 24 hours
Freeze Dried Reagents Freeze-Dried Reagents contain all components in a ready-to-use product • • Contains all reaction components Room temperature storage Reduces set-up time and minimizes operator error Cost savings in time and expertise
R. A. P. I. D. LT Software Features • Easy-to-use step-by-step Wizard User Interface
Real-Time Fluorescent PCR • Temperature cycling • Fluorimeter acquisition • Real time read out
Automated Data Interpretation • Positives results indicated by Warning dialog box • Verify by following appropriate BAM or FSIS guidelines
• Idaho Technology Assays Food Pathogen Assays available (Bio-Defense RAPID & RAZOR) – – • Salmonella E. coli O 157 Listeria monocytogenes Campylobacter Food Security System AOAC Approval Timeline – March 2008 Salmonella (AOAC-RI Approval # 030803) – January 209 Listeria spp. (AOAC-RI Approval # 010901) – Spring 2009 E. Coli O 157: H 7 • Other Assays Available -Anthrax -Avian Influenza -Plague -Tularemia -Botulism -FMDV -Ricin* -Cryptospridium -Smallpox -Brucella
Training and Customer Support • Training programs – Initial training included with system purchase – Most lab techs trained in ½ day • 24/7 technical support available • RAPID LT includes 1 year warranty
In Summary The R. A. P. I. D. LT FSS offers: • • • Complete product solution – hardware, chemistry, software and technical support Accuracy and specificity Ease of use Extensive scientific support and expertise Shortened enrichment times & faster total time to result Pooling capability, cost efficiency Only true walk-away platform on the market Freeze-dried reagents/no proprietary media Ability to run other commercially available products
Follow up Contact Idaho Technology, Inc. 390 Wakara Way Salt Lake City, Utah 84108, USA 801 -736 -6354 Main Phone Number www. idahotech. com Dennis D’Alfonso National Sales Manager 801 -230 -8614 Cellular Phone Number dennis_dalfonso@idahotech. com