Progress Update W-NAME-R Wireless Neural Activity Monitoring Environment

Progress Update: W-NAME-R Wireless Neural Activity Monitoring Environment for Rodents 01/27/12 Chaitali Biswas http: //icslwebs. ee. ucla. edu/dejan/researchwiki/index. php? title=Wireless_Neuronal_Activity_Monitoring_Environ ment_for_Rodents UCLA

Outline: 01/27/2012 w Background – Why monitor brain injured rats? w w – Invasively – Wirelessly Research Questions – Neuroscience / Neurosurgery – Neuroenginnering Design – Established / Adopted / Under Discussion / Forecasted Roadmap – Experiments & Presentations Feedback from DMGroup Progress Report: Chaitali Biswas: Jan 27 2012

Why monitor brain injured rats? w Hippocampus : = encoding + retrieval of spatial memory – Observable behavioral tasks – Learning enhanced by EEE w Injury Variables ‾ Insult Mechanics ‾ Acute Indications ‾ Chronic Indications w Hypothesis – quantifiable depression of hippocampal q – Theta [4, 12]Hz phase sets R/W access – Theta power spectrum varies across hippocampus DMGroup Progress Report: Chaitali Biswas: Jan 27 2012

Characterizing Hippocampal Theta w Spectrum [4, 12] Hz, max @ 8 Hz w Amplitude [10, 100] u. V w Time-Series Analysis: ? ? ? w Types: Attentional vs. Translational w Phase varies with – Immediately Previous Activity – Cross-talk w Injury effects – Depresses amplitude – Downshifts peak frequency MANY QUESTIONS REMAIN Bland et al, J Neurophys. 2002 DMGroup Progress Report: Chaitali Biswas: Jan 27 2012

NS Questions for W-NAME-R 1. Which stereotaxic coordinates offer max(SNR) correlated with spatial navigation during a working memory task in the uninjured rat brain? 2. Which is the minimal set of intra-hippocampal loci where theta dysfunction may be detected and reliably correlated back to abnormal working memory task performance due to injury? 3. What are differences in theta functionality between injured and uninjured subjects during (a) memory tasks (NOR/MWM/RAM) (b) exploration of enriched environment (EEE)? 4. Can theta dysfunction in injured subjects be used as quantitative predictor of malperformance in working memory tasks subsequent to EEE training? DMGroup Progress Report: Chaitali Biswas: Jan 27 2012

NE Questions for W-NAME-R 1. What is optimal type and design of electrode for chronic monitoring of hippocampal theta? 2. How many channels can be supported for raw theta [4, 12]Hz x IP 3, [6, 8]bit ADC on 5 g battery? How long on 1 change battery? 3. How to design enriched environment cage? Time? Cost? 4. How many rodents can be supported off 1 basestation transceiver? 5. How to set up camera system to co-register tracked behavior w/ theta? 6. Which algorithms correlate injury theta to abnormal navigation? 7. Design a QEEG algorithm: theta waves expected quantifiable behavior metrics DMGroup Progress Report: Chaitali Biswas: Jan 27 2012

Experiments & Presentations w Electrode Selection w Headstage Construction + Wi. Fi Testing w w w Implantation Technique w/ Tethered Recording - sham Headstage Installation - sham Novel Object Recognition (NOR) Test – sham Battery Life Test – sham Headstage Retention by Animal – sham Post-injury Implantation – TBI w Camera captured NOR behavior tracking – sham + TBI w Mapping theta to behavior – pattern detection w Presentation – ? : Neurotrauma: Abstract April 15 2012 – ? : Thesis Proposal: Fall 2012 DMGroup Progress Report: Chaitali Biswas: Jan 27 2012