Presents Multi Chrome TM TM Veterinary Microbiology

Presents

Multi. Chrome TM TM Veterinary Microbiology Culture System Gram Positive 3/10 Exp 23 Lot #1 P(+) e. G hrom Multi. C Gram Negative (-) e GP S. Aureus S. Intermedius E. faecalis E. Coli Klebsiella S. saprophyticus Pseudomonas

BACTERIA AS A PATHOGEN Bacteria are unicellular organisms that reproduce by cell division; usually have cell walls; can be shaped like spheres, rods or spirals; and can be found in virtually any environment. Although many are synergistic in organisms, they can also cause disease. In veterinary medicine we routinely see them affecting nine areas: ØOuter Ear Infections ØUrinary Tract Infections ØConjunctival infections ØGenital infections ØWound/abscess ØSkin Infections ØUpper Respiratory Infections ØMastitis ØSepticemia

CURRENT METHODS OF DETECTION AND IDENTIFICATION There are generally two steps involved in the detection, identification and treatment of bacterial pathogens ØCulture Growing suspected bacterial agents in a nutrient media (agar) within a controlled environment (incubator) ØSensitivity Subjecting bacterial agents to various common veterinary antibiotics to determine efficacy.

CURRENT METHODS OF DETECTION AND IDENTIFICATION (continued) Most veterinary clinics and hospitals for lack of a comprehensive, easy to use in-house method opt for using a reference laboratory. This involves: Ø Ø Ø Collecting a sample from the affected area Sending it to a reference laboratory Treat suspected bacteria with antibiotic Receiving the culture and/or sensitivity results in 2 -3 days Recheck in 10 days to adjust antibiotic if necessary. This comes at a great cost per test to both the veterinarian and the client with no guarantee the sample will not be deemed unusable. (Note: Up to 30% of all UTI samples sent to labs are rejected. )

Multi. Chrome TM TM Veterinary Microbiology Culture System 3/10 Exp 23 Lot #1 me G P(+) M G rome ulti. Ch hro Multi. C P(-) Multi. Chrome is cutting edge technology for Veterinary microbiology. At the center of this system is a unique Chromogenic culture bi-plate that: ØIsolates Gram Positive from Gram Negative bacteria Differentiation of bacteria into two families based on their ability to accept or reject a chromogen indicator. Gram Positive ØE. faecalis (Bone) ØS. intermedius (Ear) ØS. aureus (Skin) ØS. uberis (Mastitis) (+) (-) Exp 3/10 Lot #123 Multi. Chrome GP(+) Multi. Chrome GN(-) Gram Negative ØProteus (UTI) ØPseudomonas (Ear) ØKlebsiella (UTI) ØE. Coli (UTI 90%)

ØIs designed to ignite, individual, distinct color within bacteria Causes a reaction within the bacteria resulting in distinct colors such as in E. Coli appearing as a rose colored colony with a darker center or Strep Agalactiae appearing teal to turquoise blue, etc.

Bacterial pathogen probability charts for Canine and Feline: These reference charts suggest the highest and lowest percentage for infection of various parts of the body by common pathogenic bacteria. Once identified proceed with accepted veterinary antibiotic protocols for treatment of pathogens. Species: Canine Percentage Probability Bacteria UTI Ear Staph Intermedius 10 -15% 25 -30% E. Coli >50% 10 -20% Enterococcus faecalis <10% NGS Proteus mirabilis 15% 20 -25% Obligate anerobes NGS Pseudomonas NGS 15 -25% Klebsiella 10 -15% NGS Pasturella NGS= Not Generally Seen Bone 40 -50% 10 -20% NGS NGS Skin 60 -70% 20 -30% NGS <10% NGS Genital 15 -25% 30 -35% NGS NGS <10% NGS 10 -25% Wound 25 -35% 20 -30% NGS 10 -20% 25 -35% 10 -20% NGS Blood 25 -35% 35 -45% NGS 10 -20% 20 -35% NGS URI 30 -35% 15 -20% NGS NGS <10% 15 -20% Species: Feline Percent Probability Bacteria Conjunctival Genital Pasturella 10 -20% 15 -25% E. Coli NGS 30 -35% Proteus mirabilis NGS Pseudomonas NGS <10% Obligate anerobes NGS 10 -25% Klebsiella NGS Chlamydia psittoci 50 -75% NGS B-hemolytic strep 15 -25% NGS= Not Generally Seen UTI 10 -15% 40 -45% 10 -15% <10% NGS NGS Wound 30 -40% 10 -20% <10% NGS 25 -35% NGS NGS URI >50% 15 -20% <10% NGS NGS Skin >50% 20 -30% <10% NGS NGS NGS Probability reference charts taken from “Target: The Antimicrobial Reference Guide to Effective Treatment” , second edition, revised. Author: Dr. David Aucoin, DVM, Diplomate of the American College of Veterinary Clinical Pharmacology. Data on file, Kacey® Asheville, N. C. 28801.

Multi. Chrome TM TM Veterinary Microbiology Culture System ØProduces in-house confirmatory results in 24 hours No waiting for lab results that can take up to 3 days and STILL be deemed inconclusive or unusable. Why wait for results?

“Multi. Chrome™ Microbiology System In-House Culture Procedure Materials needed: 1) A sterile Swab or inoculating loop (10 ul recommended) 2) One Multi. Chrome plate brought to room temperature (stored in fridge) 3) Existing incubator or the NEW Multi. Chrome™ Micro Incubator Exp 3/10 Lot #123 Multi. Chrome GP(+) Multi. Chrome GN(-)

“Multi. Chrome™ Microbiology System In-House Culture Procedure (continued) Procedure: Simple, Fast & Easy! 1) 2) 3) 4) Obtain sample from affected area with a swab or loop Streak both sides of the Multi. Chrome plate in a zigzag fashion Place in pre heated incubator (98. 6 F or 37 C) Read in 24 hours Exp 3/10 Lot #123 Multi. Chrome GP(+) Multi. Chrome GN(-) Inoculated Multi. Chrome GP(+) Multi. Chrome GN(-) 24 Hour Confirmation

“Multi. Chrome™ Microbiology System In-House Culture Procedure (continued) Reading Results: Gram Positive Gram Negative S. Aureus* S. Intermedius E. faecalis E. Coli S. saprophyticus Only a few of the bacteria detected! Klebsiella Pseudomonas *Can even detect super bug MRSA with the Multi. Chrome™ MRSA specific Test plate coming in 12/09. MRSA (Methicillin Resistant Staph Aureus) has been recently identified on companion animals and is a zoonotic. Confirmation can be made first by its +/- Gram family and then by its distinctive color match to the reference chart included in each pack of Multi. Chrome™. Veterinarians can treat at this stage with the antibiotics most appropriate for the bacteria found. A re-check in ten days is recommended Confirmation and Identification in just 24 hours!

“Multi. Chrome™ Microbiology System and Sensitivity Testing We have now completed the first stage in a microbiology in-house system and we could stop there, but what about Sensitivity testing? Simply inoculate a “Mueller Hinton” agar plate with confirmed growth from the Multi. Chrome™ plate, drop in antibiotic test discs and incubate for another 24 hours. Kacey “Multi. Chrome™ Micro Incubator Sensitivity disk with antibiotic discs

Kacey Multi. Chrome™ Micro Incubator Although the Multi. Chrome™ system can be used with conventional incubators the patented Kacey Micro Incubator is far from conventional. Here is why: ØSmall footprint (8” wide, 13” high, 12” deep) ØPrecision temperature control ØDigital temperature readout ØSliding trays which cradle for all forms of dishes ØClear door for observation ØTwelve individual 24 hour countdown timers ØAudible and visible alert when sample is ready ØLightweight ABS construction, 1 year warranty ØSuitable for Multi. Chrome, Dermatophyte, Sensitivity and related diagnostics requiring incubation Easy Operation: 1 - Load Sample 2 - Press timer 3 - Walk away

Benefits of using the Multi. Chrome™ Microbiology System To best demonstrate this, let’s compare the Diagnostic and Economic benefits of Multi. Chrome™ versus the Ref. Lab. Diagnostics: Benefits Multi. Chrome™ Ref Laboratory Accuracy Within 5 -10%* Within 5 -10% Turn around time 24 hours 2 -3 days Flexibility Culture and/or sensitivity No stand alone Sensitivities accepted *Well within the acceptable error range of most labs. Accuracy will improve with practice. For unresponsive bacterial agents and/or unknown etiology we do recommend the use of a Reference lab for more advanced diagnostic tests.

Benefits of using the Multi. Chrome™ Microbiology System (continued) Economic benefit to the Veterinarian: It’s all about gross profit! Let’s compare the current reference lab prices to the Multi. Chrome™ system on one Culture & Sensitivity sample. Reference Labs $58. 00 (avg. ) Cost for C&S $116. 00 (avg. ) Client fee (2 x lab fee) $58. 00 Gross Profit Multi. Chrome™ $8. 00 (avg. ) Cost for Culture $116. 00 (avg. ) Client fee $108. 00 Gross Profit NOTE: This does not account for samples rejected by lab, but charged for and not generally recovered in the fee to the client. In that case the gross profit on a re-run would be $0. 00.

Multi. Chrome TM TM Veterinary Microbiology Culture System To recap, Multi. Chrome™ offers the following: ØSpeed: 24 hour to confirmation, identification & treatment ØConvenience: In-house ability to test 9 areas affected by bacteria ØReliability: Cutting edge technology with superior accuracy ØProfit Potential: A fraction of reference lab prices ØProfessional Curiosity: “You were trained, you just need the tools!” Multi. Chrome™ “A different way to detect bacteria!” 3/10 Exp 23 Lot #1 hrom Multi. C (+) e GP hrom Multi. C (-) e GP

Multi. Chrome TM TM Veterinary Microbiology Culture System Product details: Multi. Chrome™ is available in convenient packs of 5 or 10 plates: Five packs* Ten packs* Each plate individually packaged and sterilized Full color reference insert included with each pack Shipped refrigerated, plates thaw in 5 -10 min 5 -6 month expiration from date of shipping Multi. Chrome™ Micro Incubator*: Each incubator comes complete, ready to use with five trays *Contact your local distributor for exact pricing and for promotions or discounts if applicable. 3/10 Exp ) GP 23 Lot #1 (+ e hrom Multi. C hrome Multi. C GP(-)

1854 A Hendersonville Rd Asheville, NC 28803 Ph: 1(828)685 -3569 Fax: 1(828)685 -3571 www. kaceyinc. org “Kacey Products Providing Better Animal Care” Coming Soon in Q 2 -10! Kacey Automated Slide Stainer Kacey Diagnostics © 2010 All Rights Reserved