NATURAL FIBROUS MATERIALS AS A SOURCE OF INSPIRATION FOR THE DESIGN OF NOVEL NANOBIOMATERIALS Anna Mitraki Laboratory of Natural Biomaterials Department of Materials Science and Technology, University of Crete, and Institute for Electronic Structure and Lasers, FORTH mitraki@materials. uoc. gr
Our research focuses on: Rational design of protein fibrous materials from the nano- to the macroscopic scale using building blocks inspired from Natural fibrous folds and hard-won biochemical knowledge Crystal structure of a natural fibrous protein made from sequence repeats (blue squares) [van Raaij et al. Nature 1999, 401: 935] 50 nm
Peptide sequences as a self-assembly system Identification of minimal peptides corresponding to natural building blocks (blue squares) that can self-assemble into fibrous objects [ ]n B 50 nm 100 nm Papanikolopoulou et al. , J. Biol. Chem. 2005, 280 : 2481 -2490
Modification of fibrous building blocks towards specific functions: -targeting sequences for metallization, cell attachment etc, or -Conjugation to other organic moieties to form hybrid materials
Example: Metal nanowires fabricated on an octapeptide fibrillar scaffold displaying cysteines Kasotakis et al. , 2009, Biopolymers-Peptide Science, 92: 164 -72
…. and Development of inter-disciplinary approaches towards controlled positioning on surfaces and integration in devices Example: peptide fibrils positioned on a hybrid organicinorganic material using femtosecond Laser Technologies V. Dinca et al. , Nanoletters, (2008) 8 (2): 528 -543 A. Mitraki and M. Farsari, in : "Nanomaterials for Life Sciences" series, vol. 15, pp 105 -119, Wiley- VCH
Relevant recent publications: 1) Dinca V. , et al. , (2008) Directed three-dimensional patterning of self-assembled peptide fibrils. Nanoletters, 8: 538 -543 2) Dinca V. , et al. , (2008) Fabrication of amyloid peptide micro-arrays using Laser-induced forward transfer and avidin-biotin mediated assembly Physica Status Solidi 5: 3576 -3579 3) Kasotakis, E. , et al. , (2009) Design of metal-binding sites onto self-assembled peptide fibrils Biopolymers –Peptide Science 92: 164 -172 4) Tamamis, P. , et al. , (2009) Amyloid-like self-assembly of peptide sequences from the adenovirus fiber shaft: insights from molecular dynamics simulations. J. Phys. Chem. B. 113: 15639 -15647 5) Mitraki, A. (2010) Protein aggregation: from inclusion bodies to amyloid and biomaterials. Advances in Protein Chemistry and Structural Biology, 79: 89 -125 6) Sedman VL. , et al. , (2011) Surface-Templated Fibril Growth of Peptide Fragments from the Shaft Domain of the Adenovirus Fibre Protein Pept Lett. , 18: 268 -274 7) Tiggelaar, SM. , et al. , (2011). Neutron fibre diffraction studies of amyloid using H(2)O/D(2)O isotopic replacement. Acta Crystallogr Sect F Struct Biol Cryst Commun. 67: 332 -335. 8) Hyttel-Clausen C. , et al. , (2011) Electrostatic force microscopy of self-assembled peptide structures. Scanning, in press 9) Viguier, B. , et al. , (2011) Development of an electrochemical metal-ion biosensor using self-assembled peptide nanofibrils. ACS Appl. Mater. Interfaces, in press. 10) Charalambidis, G. , et al. , 2011) Self-assembly into spheres of a hybrid diphenylalanine-porphyrin: increased fluorescence lifetime, conserved electronic properties. Chemistry Eur. J. , in press
Acknowledgements Uo. C: Emmanouil Kasotakis Erifyli Kaloudi Dina Terzaki Alexandra Siakouli (EM Facility) Eva Papadogiorgaki (EM Facility) Aleka Manousaki (EM Facility) Collaborations - IESL, FORTH CRETE Dr. Maria Farsari, Prof. Costas Fotakis, - Technical University of Denmark (DTU) Prof. Winnie Svendsen, Dr. Jaime Castillo - Institut Laue Langevin in Grenoble, France Prof. Trevor Forsyth, Estelle Mossou - University of Nottingham, England Prof. Saul Tendler, Prof. Clive Roberts, Dr. Stephanie Allen, Dr. Sedman Victoria - University of Santiago de Compostela, Spain Financial support: Prof. Mark J. van Raaij - University of Tel-Aviv, Israel EU “Be. Natural” project Prof. Ehud Gazit, Lihi Adler-Abramovich - University of Cyprus “Bioengineered Nanomaterials For Research and Applications” Prof. G. Archontis, Dr. Ph. Tamamis - CEA Saclay, France NMP 4 -CT-2006 -033256 Dr. P. Guenoun, Cr. C. Chevallard Coordinated by Uo. C
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