MSU Skol Tech Phage and virus replication

MSU & Skol. Tech Phage and virus replication

Phage and virus replication Extreme diversity of virus genetic material and its transformations

Phage l Linear double stranded DNA genome, able to circularize

Phage l Transition from to rolling cycle mechanism of replication

Phage l Genomes separation is coupled to packaging

Phage l Lysogenic pathway: integration of phage genome to the host genome

Phage l Integration of phage genome to the host genome via covalent intermediate

Phage l Integration of phage genome to the host genome via covalent intermediate

Phage l Intasome – DNA/protein complex

Phage l Phage genome could be excised, but it requires additional protein, Xis

Phage f. X 174 Circular single stranded DNA genome, able to form circular double stranded form

Phage f. X 174 Circular single stranded DNA genome, able to form circular double stranded form

Phage f. X 174 Start of the (+) strand synthesis

Phage f. X 174 Single stranded variant of the rolling cycle replication

Phage Mu A transposon-like phage

Phage Mu Integration to the host genome

Phage Mu behaves as a replicative transposon within the host genome

Phage Mu For phage particle assembly a phage genome is cutted from the host genome with excess DNA

Retrovirus Linear single stranded (+) RNA genome, able to form linear double stranded integrated DNA form

Retrovirus Problem of LTR formation

Retrovirus LTR is necessary for transcription of the genome

Influenza virus Linear single stranded (-) RNA genome, able to form linear double stranded RNA genome and single stranded (+) form

Influenza virus Stealing the cap from host m. RNA

Influenza virus Unusual mechanism of polyadenylation

Influenza virus Replication doesn’t require caps of host m. RNA