MSU Skol Tech DNA recombination Recombination

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РЕГИСТРАЦИЯ

MSU & Skol. Tech DNA recombination

Recombination Types of recombination Homologues recombination Site specific recombination Transposition recombination

Homologues recombination Basic common steps of homologues recombination DSB 3’-overhang formation Strand invasion

Homologues recombination Basic common steps of homologues recombination Extension/ strand displacement Holliday junction Invasion of the second strand

Homologues recombination Basic common steps of homologues recombination Symmetry of the Holliday junction Strand invasion

Homologues recombination Basic common steps of homologues recombination

Homologues recombination Source of DSB Radiation/chemical DSB Single strand break in the template strand during replication

Homologues recombination Recombination in bacteria

Homologues recombination Rec. BCD motor

Homologues recombination Rec. A catalyze strand invasion

Homologues recombination in eukaryotes

Homologues recombination Activation MRN activates ATM activates Ct. IP (locally at the place of damage) CDK activated Ct. IP (globally in S or G 2 phase) BRCA 1 stimulate end resection in S or G 2, while 53 BP 1/Rif 1/PTIP inhibit it in G 1 Ct. IP activate cleavage and removal of damaged 5’end

Homologues recombination End resection Endo cleavage, 3’exo

Homologues recombination Initial cleavage by Mre 11 is followed by extensive 3’exo by two redundant pathways Rad 9 limits end resection, SMARCAD 1 displace Rad 9 and allow more extensive resection