Mash 1 -dependent Notch Signaling Pathway Regulates GABAergic Neuron -Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells Qianfa Long 1, 2 ; Qiang Luo a ; Kai Wang 3 ; Adrian Bates 2, 4 ; Ashok K. Shetty 2, 4 ; 1 Department of Neurosurgery, Xi#cod#x 02019; an Central Hospital, Xi#cod#x 02019; an Jiao Tong University School of Medicine, Xi#cod#x 02019; an 710003, China ; 2 Institute for Regenerative Medicine, Texas A#cod#x 00026; M Health Science Center College of Medicine at Scott #cod#x 00026; White, Temple and College Station, Texas, 76502, USA ; 3 Department of Neurosurgery, Qingdao 401 Hospital of PLA, Qingdao 266071, China ; 4 Research Service, Olin E. Teague Veterans#cod#x 02019; Medical Center, CTVHCS, Temple, Texas, USA ; Figure 5. Immunofluorescence showing GABAergic neuron-like differentiation from Mash 1 overexpressed BMSCs After Mash 1+ BMSCs were induced by cocktail conditioned medium, immunofluorescence showed that differentiated cells expressed neuronal cytoskeleton marker NF-H C , white arrow, mature neuronal marker Neu. N H , white arrow, and specific neuronal marker #cod#x 003 B 2; -3 tubulin M , white arrow, in addition to GABA-ergic cell marker GAD 67 D, I and N , white arrow. Note that, many GAD 67 positive cells co-localize with NF-H A E , white arrow, Neu. N F J , white arrow, and #cod#x 003 B 2; -3 tubulin K O , white arrow. B, G and L illustrate DAPI stained nuclei. Bar A, F and K = 50 #cod#x 003 BC; m, bar E, J and O = 20 #cod#x 003 BC; m. Statistical analysis shows the percentage of GABA-ergic neuron-like Aging and Disease, null, 8(3), 301 -313. Doi: 10. 14336/AD. 2016. 1018 differentiation in genetically engineered BMSCs P. Statistical differences referred to Lv-con-BMSCs, #cod#x 0002 A; P = 1. 9036 E-05, #cod#x 0003 C; 0. 01; #cod#x 0002 A; P = 3. 61045 E-05, #cod#x 0003 C; 0. 01; #cod#x 0002 A; P = 0. 122, #cod#x 0003 E; 0. 05.
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