2017 LECTURES 21 AND 223 ACTIN CYTOSKELETON AND CELL MOTILITY.ppt
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Lectures 21 and 22: The Cytoskeleton: Microfilaments Essential Cell Biology Third Edition Chapter 17
The Cytoskeleton Includes Dynamic Networks Of Microfilaments And Microfilaments
Microfilaments composed of actin are often found at the cortex of cells. They also form stress fibers that give shape to the cell. They are highly dynamic forming networks that serve as the basis for cellular motility.
Microfilaments Consist Of A Double Helix Of Actin Monomers Bound To Each Other
Microfilaments (MFs) consist of a double helix Of actin monomers Platinum coated microfilaments in a white blood cell A single microfilament in vitro
Actin Polymerization Can Occur In Vitro. No Other Proteins Are Required.
Microfilaments like microtubules have plus and minus ends that are not identical. The plus end favors polymerization. The minus end favors depolymerization. MINUS END FAVORS DISSOCIATION Minus End = “pointed end” ATP HYDROLYSIS PLUS END FAVORS ADDITION Plus End = “barbed end”
Animation: Regulation of Actin Polymerization and Depolymerization inside cells using Profilin and Cofilin Profilin binds to actin monomers (G-actin) to aid polymerization; cofilin binds to microfilaments (f-actin) to sever them and allow faster depolymerization
3 -D Microfilament Networks Are The Basis Of Dynamic Cell Structures: Network construction requires accessory proteins that interact with actin
Microfilaments Interact With accessory proteins (actin binding proteins) In Forming Networks or rapid Disassembly of networks Filamin dimer Cofillin or Gelsolin Microfilament bundling And crosslinking proteins
Microfilaments Form Bundles using the crosslinking proteins α-Actinin And Fimbrin Stress Fibers Microvilli Can shorten Can not shorten
Microvilli Contain A Microfilament Bundle
Microfilament elongation in vivo is aided by the accessory proteins profilin and formin. Profilin binds to ATP- actin and the Formin complex acts as a building block. The complex prevents nucleation. Formin binds P-A complexes and guides them to the growing (barbed) end of the Microfilament Profilin Actin
Animation of Actin Polymerization using Formin
In cells, the ends of microfilaments are capped with other proteins to control assembly. ARP 2 and ARP 3 cap the minus end of microfilaments. CAPPING PREVENTS ACTIN DISSOCIATION
ARPs allow binding of minus ends to other filaments. In this way microfilament networks can be formed that are used as superstructures for cell shape and motility.
Dynamics of Actin Networks in a slime mold.
Micro. Filament Networks Are The Basis Of Amoeboid Movement THE LEADING EDGE
Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.
Microfilament Networks Are Dynamic At The Leading Edge
Animation of ARP 2/3 induced branching of microfilaments as facilitated by WASP proteins
Gab 1 Adaptor Growth Factor Receptor Auto-inhibited WASP Cell Ruffling Response Actin polymerization and branching ARP 2/3 Complex Signal Rho GTPase Rac GTPase GEF Activated WASP G-Protein “Switch” Cell Signaling Controls The Actin Cytoskeleton Formin polymerization filopodia extend WASP ARP 2/3 branching lamellapodia
Micro. Filament Networks Are the Basis of Ameoboid Movement THE LEADING EDGE
Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.
Focal adhesions use hundreds of transmembrane proteins called integrins for linking the actin cytoskeleton inside the cell to extracellular matrix fibers such as collagen outsidethe cell. This linkage has a mechanical function. Focal adhesions are dynamically controlled allowing them to bind, and unbind from extracellular matrix fibers in a reversible manner. This allows them to serve as temporary anchors during cell movement.
Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.
Contraction of microfilament networks requires Myosin II filaments to make microfilaments slide. Myosin - II Anchored Cortical Microfilaments Rear of Cell Contracts
The ability of myosin to walk on actin filaments is due to a cycle of force producing conformational changes that is powered by ATP hydrolysis. This cycle is the same as in skeletal muscle.
Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.
Endocytosis and vesicle transport from back to front. Depolymerization And transport of actin back to front. Via microtubule network and motor proteins. Movement of nucleus, cytoplasm and organelles back to front via Myosin-induced movement along sub-nuclear stress fibers.
LAMELLAPODIA = LEADING EDGE – powered by polymerization FORMIN CONTRACTION AT REAR WASP CORTICAL ACTIN LAYER (ANCHORED) WHILE CELL ORGANELLES MOVE FORWARD ADHESION PLAQUE/ FOCAL CONTACT Soon-Tuck Sit, and Ed Manser J Cell Sci 2011; 124: 679 -683