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2017 LECTURES 21 AND 223 ACTIN CYTOSKELETON AND CELL MOTILITY.ppt

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Lectures 21 and 22: The Cytoskeleton: Microfilaments Essential Cell Biology Third Edition Chapter 17 Lectures 21 and 22: The Cytoskeleton: Microfilaments Essential Cell Biology Third Edition Chapter 17

The Cytoskeleton Includes Dynamic Networks Of Microfilaments And Microfilaments The Cytoskeleton Includes Dynamic Networks Of Microfilaments And Microfilaments

Microfilaments composed of actin are often found at the cortex of cells. They also Microfilaments composed of actin are often found at the cortex of cells. They also form stress fibers that give shape to the cell. They are highly dynamic forming networks that serve as the basis for cellular motility.

Microfilaments Consist Of A Double Helix Of Actin Monomers Bound To Each Other Microfilaments Consist Of A Double Helix Of Actin Monomers Bound To Each Other

Microfilaments (MFs) consist of a double helix Of actin monomers Platinum coated microfilaments in Microfilaments (MFs) consist of a double helix Of actin monomers Platinum coated microfilaments in a white blood cell A single microfilament in vitro

Actin Polymerization Can Occur In Vitro. No Other Proteins Are Required. Actin Polymerization Can Occur In Vitro. No Other Proteins Are Required.

Microfilaments like microtubules have plus and minus ends that are not identical. The plus Microfilaments like microtubules have plus and minus ends that are not identical. The plus end favors polymerization. The minus end favors depolymerization. MINUS END FAVORS DISSOCIATION Minus End = “pointed end” ATP HYDROLYSIS PLUS END FAVORS ADDITION Plus End = “barbed end”

Animation: Regulation of Actin Polymerization and Depolymerization inside cells using Profilin and Cofilin Profilin Animation: Regulation of Actin Polymerization and Depolymerization inside cells using Profilin and Cofilin Profilin binds to actin monomers (G-actin) to aid polymerization; cofilin binds to microfilaments (f-actin) to sever them and allow faster depolymerization

3 -D Microfilament Networks Are The Basis Of Dynamic Cell Structures: Network construction requires 3 -D Microfilament Networks Are The Basis Of Dynamic Cell Structures: Network construction requires accessory proteins that interact with actin

Microfilaments Interact With accessory proteins (actin binding proteins) In Forming Networks or rapid Disassembly Microfilaments Interact With accessory proteins (actin binding proteins) In Forming Networks or rapid Disassembly of networks Filamin dimer Cofillin or Gelsolin Microfilament bundling And crosslinking proteins

Microfilaments Form Bundles using the crosslinking proteins α-Actinin And Fimbrin Stress Fibers Microvilli Can Microfilaments Form Bundles using the crosslinking proteins α-Actinin And Fimbrin Stress Fibers Microvilli Can shorten Can not shorten

Microvilli Contain A Microfilament Bundle Microvilli Contain A Microfilament Bundle

Microfilament elongation in vivo is aided by the accessory proteins profilin and formin. Profilin Microfilament elongation in vivo is aided by the accessory proteins profilin and formin. Profilin binds to ATP- actin and the Formin complex acts as a building block. The complex prevents nucleation. Formin binds P-A complexes and guides them to the growing (barbed) end of the Microfilament Profilin Actin

Animation of Actin Polymerization using Formin Animation of Actin Polymerization using Formin

In cells, the ends of microfilaments are capped with other proteins to control assembly. In cells, the ends of microfilaments are capped with other proteins to control assembly. ARP 2 and ARP 3 cap the minus end of microfilaments. CAPPING PREVENTS ACTIN DISSOCIATION

ARPs allow binding of minus ends to other filaments. In this way microfilament networks ARPs allow binding of minus ends to other filaments. In this way microfilament networks can be formed that are used as superstructures for cell shape and motility.

Dynamics of Actin Networks in a slime mold. Dynamics of Actin Networks in a slime mold.

Micro. Filament Networks Are The Basis Of Amoeboid Movement THE LEADING EDGE Micro. Filament Networks Are The Basis Of Amoeboid Movement THE LEADING EDGE

Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.

Microfilament Networks Are Dynamic At The Leading Edge Microfilament Networks Are Dynamic At The Leading Edge

Animation of ARP 2/3 induced branching of microfilaments as facilitated by WASP proteins Animation of ARP 2/3 induced branching of microfilaments as facilitated by WASP proteins

Gab 1 Adaptor Growth Factor Receptor Auto-inhibited WASP Cell Ruffling Response Actin polymerization and Gab 1 Adaptor Growth Factor Receptor Auto-inhibited WASP Cell Ruffling Response Actin polymerization and branching ARP 2/3 Complex Signal Rho GTPase Rac GTPase GEF Activated WASP G-Protein “Switch” Cell Signaling Controls The Actin Cytoskeleton Formin polymerization filopodia extend WASP ARP 2/3 branching lamellapodia

Micro. Filament Networks Are the Basis of Ameoboid Movement THE LEADING EDGE Micro. Filament Networks Are the Basis of Ameoboid Movement THE LEADING EDGE

Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.

Focal adhesions use hundreds of transmembrane proteins called integrins for linking the actin cytoskeleton Focal adhesions use hundreds of transmembrane proteins called integrins for linking the actin cytoskeleton inside the cell to extracellular matrix fibers such as collagen outsidethe cell. This linkage has a mechanical function. Focal adhesions are dynamically controlled allowing them to bind, and unbind from extracellular matrix fibers in a reversible manner. This allows them to serve as temporary anchors during cell movement.

Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.

Contraction of microfilament networks requires Myosin II filaments to make microfilaments slide. Myosin - Contraction of microfilament networks requires Myosin II filaments to make microfilaments slide. Myosin - II Anchored Cortical Microfilaments Rear of Cell Contracts

The ability of myosin to walk on actin filaments is due to a cycle The ability of myosin to walk on actin filaments is due to a cycle of force producing conformational changes that is powered by ATP hydrolysis. This cycle is the same as in skeletal muscle.

Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to Steps In The Cycle Of Amoeboid Movement 1. Extension of leading edge due to actin Polymerization. 2. Linking of cell cortex to substratum via connection of cortical microfilaments to Adhesion plaques/focal contacts. 3. Rear of cell contracts and moves forward by interaction of microfilaments with myosin. 4. Microfilaments depolymerize at rear of cell. Plasma membrane is retrieved by endocytosis. Actin monomers and membrane vesicles move to front of cell via microtubules tracks to be reutilized.

Endocytosis and vesicle transport from back to front. Depolymerization And transport of actin back Endocytosis and vesicle transport from back to front. Depolymerization And transport of actin back to front. Via microtubule network and motor proteins. Movement of nucleus, cytoplasm and organelles back to front via Myosin-induced movement along sub-nuclear stress fibers.

LAMELLAPODIA = LEADING EDGE – powered by polymerization FORMIN CONTRACTION AT REAR WASP CORTICAL LAMELLAPODIA = LEADING EDGE – powered by polymerization FORMIN CONTRACTION AT REAR WASP CORTICAL ACTIN LAYER (ANCHORED) WHILE CELL ORGANELLES MOVE FORWARD ADHESION PLAQUE/ FOCAL CONTACT Soon-Tuck Sit, and Ed Manser J Cell Sci 2011; 124: 679 -683