Laboratory Methods in Biotechnology Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety
Idea Behind the Course n n Provide practical experiences in laboratory activities that will help students make the transition from content-based lower division courses to application orientated senior level courses. Provide skills and dispositions needed to carry out laboratory research as part of a career in the life sciences. This includes undergraduate research, working as a technician, graduate research, and affiliated professions.
Role in Your Curriculum Core: Reasoning, Investigation, Cell Biology, Genetics, MCMB Specialty: Lab Methods, Micro, Molecular Genetics Electives: Molecular Techniques, Cell and Molec, Immunology, Path Micro, Microbial Phys, etc
Objectives n mg mmol m. M To give students experience in dimensional analysis used in microbiology and cellular and molecular biology, and biotechnology
Objectives n To familiarize the students with theory and practice of preparing solutions from various starting materials.
Objectives n To introduce the students to means of preparing sterile media and solutions.
Objectives n To allow students to practice quantifying bacteria and viruses, and to measure the growth rates of bacterial populations.
Objectives n To allow students to quantify proteins, nucleic acids and other chemicals in solution.
Objectives n To introduce students to commonly used devices including, but not limited to: microliter pipetters, centrifuges, spectrophotometers, p. H meters, etc.
Objectives n To introduce the students to the use of enzymes for end-point determination and to the measurement of enzyme activity.
Objectives n To give students experience in the techniques for the manipulation and electrophoretic separation of proteins and DNA.
Objectives n To provide information regarding biological, chemical, radiation, and other aspects of lab safety.
Textbooks n Make sure you bring this each time. n n n Seidman and Moore – our main text Leboffe and Pierce – illustration of techniques Also need: n n Calculator! Lab Notebook
Additional Resources n n n Brock – background information on theory Day – advice on writing (humor) Robyt - Source of many handouts/additional readings CBE Manual – Style guide for preparation of manuscripts Don’t buy these
Webpage http: //cstl-csm. semo. edu/champine/BI 245/index. html n n n n Lab Activities Lecture Topics Homework Assignments In Class Assignments Manuscripts Notebook Guidelines Syllabus Grade Book
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