KAIST Bio-Applications using Metal Nano-scale structure in Microfluidic Chip Su Kyeong Kim Presentation tile Nanobiotechnology for biochemical engineers
KAIST Contents Introduction SERS in a Microfluidic Chip New Concept : Photothermal Effect Cell Lysis using Photothermal Effect Integrated with Microfluidic Chip Discussion & Conclusion References Presentation tile Nanobiotechnology for biochemical engineers
Introduction KAIST Metal Nanostructures SERS (Surface-Enhanced Raman Scattering) SPR (Surface Plasmon Resonance) laser SERS Optical Property Surface Plasmon Optical property of metal nanostructure can make possible to label-free sensing Presentation tile J. Raman Spectrosc. 2007; 38: 896– 902 Molecular ‘Fingerprint’ Nanobiotechnology for biochemical engineers
KAIST Integration with SERS and Microfluidics Biosensor Microfluidics Using metal nanostructure Label-free sensing Sensitive and selective detection SERS Integration Powerful technology Small amount of sample and reagent Time saving High resolution Multichannel Microfluidic chip Detection/identification of various analytes simultaneously Appl. Spectrosc. Vol. 61, No 10, 2007 Luke P. Lee group. Lab Chip, 2009 Presentation tile Nanobiotechnology for biochemical engineers
KAIST SERS in a Microfluidic Chip Appl. Spectrosc. Vol. 61, No 10, 2007 Multiplexed Microfluidic SERS using multiplexed microfluidics(MMFs) Advantages of MMFs system - High-throughput - Sensitive detection or identification of biomolecules limit of concentration : the low n. M and sub-p. M ranges - Analysis of various analytes under easily manipulated conditions - Reproduciblity High-sensitivity (5 x 10 -9 M) Left : Working curve of crystal violet right : pesticide(mitoxantrone) using MMF-SERS (1 x 10 -12 M) Presentation tile Reproducibility of the MMF-SERS signal of 1. 0 x 10 -6 M crystal violet Nanobiotechnology for biochemical engineers
SERS in a Microfluidic Chip KAIST SERS in CD-based platform Luke P. Lee group. Lab Chip, 2009 SERS via an optofluidic CD chip The key points of this system - Precipitation of gold nanoparticles with repeating ‘filling-drying’ Amplifications of SERS - High-sensitivity - Large-area uniform SERS substrates (a) SERS spectra of 500 n. M R 6 G molecules as a function of the filling–drying cycle by our optofluidic SERS-CD platform. (b) SERS signal at 1509 cm− 1 of R 6 G molecules with concentrations from 1 µM to 1 n. M. Presentation tile Nanobiotechnology for biochemical engineers
KAIST Chacteristics of Metal Nanostructures SERS (Surface-Enhanced Raman Scattering) SPR (Surface Plasmon Resonance) laser SERS Optical Property Surface Plasmon J. Raman Spectrosc. 2007; 38: 896– 902 Photothermal Effect laser Molecular ‘Fingerprint’ Nano Lett. , Vol. 8, No. 1, 2008 Presentation tile Nanobiotechnology for biochemical engineers
Photothermal Effect KAIST Photothermal Effect Mechanism The heat generation Q Metal nanoparticles can generate heat under optical illumination. Nanotoday FEBRUARY 2007 | VOLUME 2 | NUMBER 1 1. The laser electric field strongly drives mobile electrons inside the metal nanostructures 2. The energy gained by electrons turns into heat 3. The heat diffuses away from the nanocrystal Targeted Photothermal Lysis of Pathogenic Bacteria Nano Lett. , Vol. 8, No. 1, 2008 Only cells with gold nanorods under NIR exposure decrease in cell viability (75%) due to photothermal effect Left : Viability of Pseudomonas aeruginosa cells with attached gold nanorods following exposure to NIR light Right : the quantified LIVE/DEAD data Presentation tile Nanobiotechnology for biochemical engineers
KAIST Noble Metal Nanostructures Nanograil Template for Photothermal Cell Lysis LSPR(Localized SPR) + Photothermal Effect It is possible to control two plasmon resonance by changing the D 1 and D 2. Integrated microfluidic chip Advantages of this system - Possibility of single cell analysis - Small amount of sample - Localization of cells geometically -Staphylococcus aureus subsp. Aureus FDA strain PCI 706 (St. Elizabeth 706) Presentation tile LSPR wavelength : 726 nm Nanobiotechnology for biochemical engineers
Discussion KAIST Your own idea or suggestions for the topics!!! Injection 1 st capture molecule Apply voltage 1 st electrode SPR Apply voltage 2 nd electrode & 2 nd molecule injection ü Automated system EIS Various types of capture molecules (oligonucleotides, proteins, cells or small molecule recognizing chemicals) can be immobilized automatically. ü The same chamber could be used for label-free target biomolecule detection system : Surface Plasmon Resonance (SPR) or Electrochemical Impedance Spectroscopy (EIS) Presentation tile Nanobiotechnology for biochemical engineers
KAIST Conclusion 1. Metal nanostructures provide various bio-applications such as SERS or SPR due to their unique optical property, surface plasmon. 2. SERS integrated in multichannel microfluidic chip; multiplexed microfluidic chip and CDplatform. 3. SERS in microfluidic chip exhibit high-throughput, high-sensitivity, time saving, multi-anlytes detection and small amount of samples and reagents. 4. Photothermal effect using SPR is new concept of physical property of metal nanostructure. 5. It is possible to lyse cells or damage targeted cancer cells by using photothermal effect. 6. In microfluidic chip, photothermal cell lysis can extract cell components we want and then real time PCR is performed in one chamber. 7. I suggest that noble metal nanostructure, gold nanograil, can provide as a template for photothermal cell lysis. Presentation tile Nanobiotechnology for biochemical engineers
KAIST References 1. R. Sean Norman, John W. Stone, Anand Gole, Caterine J. Murphy, and Tara L. Sabo-Attwood, Nano Lett. , 2008, 1, 302 -306 2. Alexander O. Govorov and Hugh H. Richardson, nanotoday, 2007, 1, 30 -38 3. Kwang Ho Cheong, Dong Kee Yi, Jeong-Gun Lee, Jong-Myeon Park, Min Jun Kim, Joshua B. Edel and Christopher Ko, Lap Chip, 2008, 8, 810 -813 4. Dukhyun Choi, Taewook Kang, Hansang Cho, Yeonho Choi, and Luke P. Lee, Lap Chip, 2009 5. NAHLA A. ABU-HATAB, JOSHY, F. JHON, JENNY M. ORAN, and MICHAEL J. SEPANIAK, Appl. Spectrosc. , 2007, 10, 1116 -1121 Presentation tile Nanobiotechnology for biochemical engineers
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