Introducing automated TM ELISA — Chem Well Geoffrey

Introducing automated TM ELISA - Chem. Well Geoffrey Anderson Unitech Scientific LLC Lakewood, CA Visit us: www. unitechscientific. com

TM Chem. Well Wine Autoanalyzer Manufactured by Awareness Technology Unitech Scientific - Exclusive Distributor

First analyzer in the world to combine biochemistry and microplate EIA into one instrument. Chem. Well uses microwells for all reactions, chemistry and EIA.

A Chem. Well Tour 2 syringe pumps Optical system Wash head Windows 95, 98 or XP DI water Wash and rinse bottles Reagents Samples reactions

Chem. Well Recommended workload Designed for labs doing: 100 - 1000 tests per day or 400 EIA per 8 hours

Chem. Well Features & Benefits • Easy to Use • Continuous Sample Loading • “Stat” sample priority • Low Operating Cost • Intuitive, Open Software • Proven, Reliable Technology

Calibration Options Cut-off (Semi Qualitative) n Multi-point Polynomial fit n Cubic Spline fit. . . and more Store Calibration Curves Wine Controls: Chart Levy Jennings n

Chem. Well runs ELISA as well as Enzymatic reagents Unitech offers Histamine ELISA kits Botrytis and Aspergillus ELISA kits are in development So let’s take a quick review of the steps and principals of ELISA reagents.

Immuno Complex: an Antigen with its matching Antibody Ag Ab An antigen and its antibody fit together like 3 dimensional puzzle pieces.

Diagram of a simple ELISA with a positive sample (sample has Ag in it) n Mix the sample and diluent together in a microwell that is pre-coated with specific Ab n Incubate for x minutes until the Ag in the sample binds to the Ab on the well walls. Start with an Ab-coated well Ab Ab Add the sample with some Ag in it Ab Ab Ab Ag Ag Ab Incubate until complexed Ab-Ag Ab Ab

Conjugate is a specific Ab bound to an Enzyme. Wash out the remaining sample Ab-Ag Ab Add a conjugate Ab-Ag Ab-E Ab-Ag Incubate until complexed Ab-Ag-Ab-E Ab-Ag-Ab-E The conjugate has a second antibody to the same antigen, allowing you to make an antigen sandwich (with antibody bread). It also leaves an enzyme hanging off.

Washing. . . removes the unbound conjugate, without removing bound antibodies. Wash out the remaining conjugate Ab-Ag-Ab-E Ab Ab-Ag-Ab-E Add a substrate S Ab-Ag-Ab-E Ab S S Substrate + Enzyme produces color Ab-Ag-Ab-E-S Ab Ab-Ag-Ab-E S Ab-Ag-Ab-E-S The more Enzyme available, the more color will develop. Many ELISA’s use a stopping solution to end the color generating step before reading.

Reliable Results: Enzymatic and ELISA n n Precise pipetting Washes probe inside and out Liquid sensing Temperature controlled

Easy to Maintain n Proven Reliability, over 500 sold Self Diagnosis Phone/email support by Unitech n Modular Design n n

Economical to Use n Dramatically reduces LABOR u Laboratory u Administrative

Warranty n n 1 -year parts & labor Warranty Extended Warranty available u 6 -Month Preventative Maintenance u Free replacement of warranteed parts u Other repairs if required u Loaner instrument within 72 hours

Easy to own n n Price start at $27, 500 Reagent rack cooling option Add $2000

Chem. Well Distributed by Unitech Scientific