INNOVATIONS IN EMERGING POLLUTANTS OCEAN MONITORING USING BIOSENSORS Dr. Ing. L. Sanfilippo – SYSTEA S. p. A. , Italy
Integrated water quality monitoring system SMS developed an automated networked system to monitor in situ and in real-time marine water chemical and ecological status in coastal areas Okadaic acid Domoic acd Saxitoxin Sequential automated methods improved in µMac-Smart instrumental prototype ALGAL TOXINS Alexandrium minutum Dynophisis Psseudo Nitschia Automated method developed and under integration into µMac-Smart instrumental prototype after automatic sampling, preconcentration and cells lysis TOXIC ALGAL SPECIES Sulphonamides Automated method improved into µMac-Smart & WIZ probe prototypes and coupled with automatic sampling and Solid Phase Extraction preconcentration PHARMACEUTICALS Ammonia Nitrate Nitrite Orthophosphate WIZ multiparametric nutrient probe for these nutrients was extensively tested on the coastal buoy NUTRIENTS FLOATING PLATFORM LA SPEZIA - ITALY Modular measurement prototypes: WIZ probe COASTAL BUOY PIRAN - SLOVENIA Coordinator Prof. Giuseppe Palleschi Università degli Studi di Roma Tor Vergata giuseppe. palleschi@uniroma 2. it http: //www. project-sms. eu
ALGAL TOXINS DETECTION IN SEA WATER PRINCIPLE
ALGAL TOXINS DETECTION IN SEA WATER µMac-Smart module based on Enzyme Linked Immuno-Magnetic Optical (ELIMO) competitive assays for the quantitative sequential measurement of: Okadaic acid Domoic acid Saxitoxin. Typical reaction volume: 500 µL Minimum reagents volume: 20 µL The automated measurement module is capable to measure unattended up to 50 water samples.
AUTOMATED ALGAL TOXINS DETECTION
TOXIC ALGAE DETECTION IN SEA WATER PRINCIPLE
TOXIC ALGAE DETECTION IN SEA WATER µMac-Smart module based on Enzyme Linked Immuno. Magnetic Optical (ELIMO) assays is able to detect microalgae from genera Alexandrium, Dinophysis and Pseudo-nitzschia The water sample is automatically pretreated by a separated automated module performing cells preconcentration and lysis. Typical sample volume: 200 µL Minimum reagents volume: 20 µL
CELLS PRECONCENTRATION AND LYSIS AUTOMATED MODULE Cells pre-concentration using an hollow fibre filter, 0. 1 microns cut-off, specifically tailored for the application Concentration factor x 2000 Reuse: 10 x without loosing recovery Cells lysis of the pre-concentrated water sample by ultrasounds Positive tests with lysate able to detect down to 5000 cells / L of Alexandrium minutum spiked in environmental sample (actual LOD). Dimensions: 50 (H) x 40 (W) x 20 (D) cm
Calibration tests Spiked Sea. Water with Alexandrium minutum cells
TOXIC ALGAE DETECTION IN SEA WATER Laboratory tested @ SYSTEA (Dec-Mar) Laboratory tests @ MICROBIA (April-May) µMac-Smart for Toxic Algae Detection SYS Tests with prototype MIC Manual Test
Fully automated Integrated system for toxic algae detection: From pre-concentration to detection + • Detection of Alexandrium minutum (AM) and Pseudo. Nitzchia spp. (PN) in Seawater Lysates • Shown in equivalent cell numbers
SULPHONAMIDE DETECTION IN SEA WATER + To achieve sensitive environmental concentrations Solid Phase Extraction automated preconcentration device is still under R&D
NUTRIENTS DETECTION IN SEA WATER WIZ in-situ multiparametric probe was installed in the Vida coastal buoy, to measure nutrients (NH 3, NO 3+NO 2, NO 2 and PO 4), including 0. 1 µm cutoff automatic filtration & autocleaning
NUTRIENTS DETECTION IN SEA WATER http: //nitlab. inf. uth. gr/ smsgui/charts. html
INTEGRATED WATER QUALITY MONITORING SYSTEM AND DATA MANAGEMENT Local data acquisition & remote communication 3 G / UMTS Wi. Fi to shore side gateway
INTEGRATED MONITORING SYSTEM AND DATA MANAGEMENT (2) A large coastal water buoy already in operation in Piran (Slovenia) is hosting the equipment to measure the following parameters: Toxic algae species (Alexandrium Minutum, Pseudonitzschia or Dinophysis) The field monitoring data are accessible from a remote Web server Dissolved nutrients (NH 3, NO 2, PO 4) by WIZ in-situ probe + 0. 1 µm cut-off filtration & autocleaning Standard water quality parameters (Temperature, EC, p. H, DO, Turbidity, Chlorophyll)
INTEGRATED WATER QUALITY MONITORING SYSTEM AND DATA MANAGEMENT (3) The field monitoring data are going to be available in the same remote Web server A relocatable floating platform is going to be deployed next week in the bay of La Spezia (Italy), for field monitoring of the following dissolved compounds (after 0. 1 µm cut-off filtration with automatic cleaning by filtered sample backwash): Domoic acid, Okadaic acid and Saxitoxin Standard water quality parameters (Temperature, EC, p. H, DO, Turbidity, Chlorophyll)
CONCLUSIONS Innovative analytical methods were developed and fully automated, in the frame of the European funded research project SMS (http: //www. èrpject-sms. eu/), to measure toxic algae, their related algal toxins and sulfonammides, then coupled with nutrients detection in coastal water Toxic algae and algal toxins methods are based on the same ELIMO µLFR analytical technology Field tests are just starting in Slovenia and Italy and they will run until the beginning of August Lab data comparisons and validation are going to be performed during the field tests The ELIMO analytical concept allows its adaptation to detect other chemical and biological targets for coastal and even fresh water.
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