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Importance of Monocyte Chemoattractant Protein-1 Pathway in Neointimal Hyperplasia After Periarterial Injury in Mice Importance of Monocyte Chemoattractant Protein-1 Pathway in Neointimal Hyperplasia After Periarterial Injury in Mice and Monkeys by Kensuke Egashira, Qingwei Zhao, Chu Kataoka, Kishou Ohtani, Makoto Usui, Israel F. Charo, Ken-ichi Nishida, Shujiro Inoue, Makoto Katoh, Toshihiro Ichiki, and Akira Takeshita Circulation Research Volume 90(11): 1167 -1172 June 14, 2002 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. Histopathology and immunohistochemistry of the cuffed femoral arteries. Kensuke Egashira et al. Figure 1. Histopathology and immunohistochemistry of the cuffed femoral arteries. Kensuke Egashira et al. Circ Res. 2002; 90: 1167 -1172 Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. RT-PCR analysis for MCP-1 and CCR 2. Kensuke Egashira et al. Circ Figure 2. RT-PCR analysis for MCP-1 and CCR 2. Kensuke Egashira et al. Circ Res. 2002; 90: 1167 -1172 Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. Effect of the intramuscular transfer of the 7 ND gene on inflammation Figure 3. Effect of the intramuscular transfer of the 7 ND gene on inflammation (A) and proliferation (B) 7 days after cuff placement as well as intimal area (C), intima/media ratio (D), and percent luminal stenosis (E) 21 days after cuff placement in the mice transfected with empty plasmid or 7 ND plasmid. *P<0. 01 vs the empty plasmid treatment. Kensuke Egashira et al. Circ Res. 2002; 90: 1167 -1172 Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. RNase protection assays for various inflammatory cytokines (TNFα, IL-6, IL-1β), chemokines (MCP-1 Figure 4. RNase protection assays for various inflammatory cytokines (TNFα, IL-6, IL-1β), chemokines (MCP-1 and RANTES), and chemokine receptors (CCR 1, CCR 3, and CCR 2). Kensuke Egashira et al. Circ Res. 2002; 90: 1167 -1172 Copyright © American Heart Association, Inc. All rights reserved.

Figure 5. Effect of the intramuscular transfer of the 7 ND gene on intimal Figure 5. Effect of the intramuscular transfer of the 7 ND gene on intimal area (A), intima/media ratio (B), and percent luminal stenosis (C) 21 days after cuff placement in the monkey transfected with empty plasmid or 7 ND plasmid. *P<0. 01 vs the empty plasmid treatment (n=6 to 7). Kensuke Egashira et al. Circ Res. 2002; 90: 1167 -1172 Copyright © American Heart Association, Inc. All rights reserved.

Figure 6. Inflammation (A) and proliferation (B) 7 days after cuff placement as well Figure 6. Inflammation (A) and proliferation (B) 7 days after cuff placement as well as intimal area (C), intima/media ratio (D), and percent luminal stenosis (E) 21 days after cuff placement in wild-type mice (CCR 2+/+) and CCR 2 -deficient mice. *P<0. 01 vs the empty plasmid treatment (n=6 to 7). Kensuke Egashira et al. Circ Res. 2002; 90: 1167 -1172 Copyright © American Heart Association, Inc. All rights reserved.