Imaging Flow. Cytobot (IFCB) Marc Suddleson NOAA Ocean ServiceNCCOS Center for Sponsored Coastal Ocean Research Quay Dortch, Contributor Gregory Doucette, Contributor NOS/NCCOS Jenifer Rhoades, Contributor NOS/IOOS July 27, 2016 As presented at the 2016 NOAA Emerging Technologies Workshop Emerging Technologies
Imaging Flow. Cytobot Development and Status Emerging Technologies Active NOAA Program Investments NOSNCCOS HAB Research IOOS Ocean Tech Transfer OAR Sea Grant M. I. T. & TX NMFS Saltonstall-Kennedy Partnerships Universities, States, Companies Woods Hole Oceanographic Institution Texas A&M and U. C. Santa Cruz Texas State Departments of Health Services and Wildlife Mc. Lane Research Laboratories, Inc. Catalina Sea Ranch, LLC (new) Harmful algae Water quality New platforms (e. g. AUV) Offshore aquaculture IFCB Status • • • Continuous, autonomous ID & counts of phytoplankton, especially HABs 10 yrs of shipboard, pier-based, and cabled underwater deployments Data supporting NOAA and state science and management needs First generation commercially available New applications and next generation formats emerging As presented at the 2016 NOAA Emerging Technologies Workshop
Imaging Flow. Cytobot Description – An automated underwater microscope • • Emerging Technologies Combines lab instruments in a “can” (microscope and flow cytometer) Enables rapid ID and counting of micro-sized cells Detects chlorophyll in cells to trigger phytoplankton images Compiles images of cells in a 5 ml water sample, every 20 minutes. Up to 30, 000 high resolution cell images per hour possible. Extended, unattended deployments (6+ months) Software identifies species and counts cells as well as humans As presented at the 2016 NOAA Emerging Technologies Workshop (Olson and Sosik 2007)
Imaging Flow. Cytobot Early Successes –IFCB Deployments Emerging Technologies Support Monitoring, Forecasting & Early Warning Missions Harmful Algal Blooms, Water Quality, Ocean Observing, Aquaculture 6 San Francisco Bay Eco. Mon (NMFSCINAR) IFCB AV (IOOS OTT) Nauset Salt Marsh (Sea Grant) Catalina Sea Ranch Martha’s Vineyard Observatory (NMFS) (NSF, ONR) Current Pending Texas Coast (NCCOS PCMHAB) As presented at the 2016 NOAA Emerging Technologies Workshop IFCB AUV (IOOS OTT) 4
Imaging Flow. Cytobot Early Success #1 – Texas HAB Early Warning Emerging Technologies Reduces Harmful Algal BIoom Impacts • Efficient and Effective Shellfish Monitoring 7 HAB early warnings since 2007 • Confidence in Shellfish Product Safety No shellfish recalls since 2008. Dinophysis spp. Diarrhetic Shellfish Poisoning IFCB on UTMSI Pier, Port Aransas (L. Campbell) Karenia spp. Neurotoxic Shellfish Poisoning As presented at the 2016 NOAA Emerging Technologies Workshop
Imaging Flow. Cytobot Early Success #1 – Texas HAB Early Warning Emerging Technologies HAB Shellfish Harvesting Closures March 7 -April 12, 2008 Oyster Festival in Fulton, TX March 7 -9, 2008 As presented at the 2016 NOAA Emerging Technologies Workshop 6
Imaging Flow. Cytobot Early Success #2 – Advancing IFCB for Ocean Observations Emerging Technologies In situ measurement of phytoplankton cells and toxins solar panels IFCB 2 G ESP generator Credit: Brosnahan (WHOI) IFCB and 2 G ESP in Nauset Marsh, Cape Cod Spring 2016 As presented at the 2016 NOAA Emerging Technologies Workshop IFCB powered by solar panels and generator
Imaging Flow. Cytobot Future Directions - New Advances and Applications Emerging Technologies Staining IFCB - enhanced detection of protozoan taxa - live cell staining, targeting micrograzers Credit: Sosik (WHOI) IFCB for autonomous surface vehicles - enable spatial sampling Aquaculture Monitoring Program - add IFCB to Catalina Sea Ranch Ocean monitoring As presented at the 2016 NOAA Emerging Technologies Workshop
Imaging Flow. Cytobot Impacts/Benefits of Sustained Phytoplankton Observations Coastal Intelligence for NOAA Missions Ecological Forecasting • Understand Predict Change • Need for Operational HAB Observing System • Verify and Improve HAB Forecasts Ecosystem Monitoring and Assessment • NOAA ecosystem fisheries surveys Coastal Resilience • Determining ocean ecosystem responses to stressors (e. g. water quality) As presented at the 2016 NOAA Emerging Technologies Workshop Emerging Technologies
Imaging Flow. Cytobot IFCB Information & Resources Emerging Technologies ● NOAA HAB Research Programs – HAB application development https: //coastalscience. noaa. gov/research/habs/default ● IOOS OTT Program – observing system sensor development https: //ioos. noaa. gov/project/ocean-technology-transition/ ● Dr. Heidi Sosik, WHOI – IFCB and application designer http: //www. whoi. edu/sbl/lite. Site. do? article. Id=11906&litesiteid=7993 Olson, R. J. and H. M. Sosik. 2007. A submersible imaging-in-flow instrument to analyze nano- and microplankton: Imaging Flow. Cytobot. Limnology and Oceanography: Methods. 5: 195 -203. ). ● Dr. Lisa Campbell, Texas A&M – HAB early warning & ecology http: //ocean. tamu. edu//people/faculty/campbelllisa ● Mc. Lane Research Laboratories, Inc. – vendor, user support http: //mclanelabs. com/ ● IFCB Dashboard – publicly-available data http: //ifcb-data. whoi. edu/ As presented at the 2016 NOAA Emerging Technologies Workshop
Imaging Flow. Cytobot Acknowledgements/IFCB support As presented at the 2016 NOAA Emerging Technologies Workshop Emerging Technologies
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