i gene region Inhibitor gene Regulatory region p

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РЕГИСТРАЦИЯ

i gene region Inhibitor gene Regulatory region p O Gene 1 RNA polymerase repressor Structural genes region Gene 2 Gene 3

silencer enhancer promoter gene coding region silencer enhancer

protein A trans-acting protein trans-acting factor trans-regulation cis-regulation protein B cis-acting protein

i gene region regulatory region Inhibitor gene C p. O base pair: 1000 bp 100 bp lactose glucose galactose structural genes region Gene Z 3520 bp Gene Y Gene A 760 bp 810 bp

Catabolite gene activation protein site RNA polymerase binding region or promoter region 5’ TATAAT 3’ Pribnow box Operon region 20 bp

p z y a p galactose m. RNA

5’ 3’ RNA polymerase 1 at glucose presents at glucose absents

RNA polymerase 5’ 3’ inhibitor gene 0 galactose

RNA polymerase 5’ 3’ inhibitor gene 0

5’ 5’

5’…GCCGCCAGTTCGGCTGGCGGCATTTT… 3’ 5’…GCCGCCAGUUCGGCUGGCGGCAUUUU… 3’ U G U 5’ G A C C G C U G G C A U U-OH 3’

p Attenuater Anthranilic acid Chorismic acid Indolglycerol phosphate Tryptophan

at a lot of tryptophans present p Trp Inactive repressor Repressor at a few tryptophans present or tryptophans absent p RNA polymerase whole m. RNA Inactive fragmentary m. RNA repressor

M K A I M K A F LKG 1 2 3 4 Try code I F LK GW W R 1 Try code 3 2 4

Antisens RNA Protein

RNA ds. DNA solenoid transcription bubble chromatid supersolenoid chromosome nucleosomes

RNA polymerase

RNA polⅠ r. RNA polⅠ

TF Ⅲ B TF Ⅲ C RNA pol Ⅲ t. RNA

TF Ⅲ B TF Ⅲ A RNA pol Ⅲ TF Ⅲ C 5 s r. RNA

silencer enhancer promoter gene coding region silencer enhancer

enhancer core sequence

Gene regulatory proteins transcription initiation complex RNA pol II 5’ Enhancer-2 3’ Enhencer-1 gene TATA box Enhancer-3 +10 kb to +50 kb

regulatory protein promoter transcription initiation complex

enhancer promoter

Gene regulatory proteins transcription initiation complex RNA pol II 5’ 3’ enhancer gene various control element TATA box silencer +10 kb to +50 kb

TF Molecular weitht, k. D Function TF II A TF II B 12, 19, 35 33 stabilize TF II D TBP/38, TAP/15 -250 TF II E TF II F TF II H 30, 74 35 -89 TF II I 120 accelerate that RNA pol II binding with DNA. recognize TATA box ATPase helicase phosphorylation of carboxyl terminal domain of RNA pol II large subunit accelerate that TF II D binding TATA

C C C Zn H C H H H COOH

O H N Leucine residue C C CH COOH 1 1 8 8 15 15 22 22 29 29 36 36 COOH

3 2 2 1 3 2 1 1 3 2 2 3 3 2 3 1

2 3 1

Poly A site initiation site upstream silencer AATAAA, 30 bp ATG TAA downstream enhancer 5’ 1 1 2 2 n-1 terminator upstream promoter enhancer GC box CAAT box n TATA box coding 3’ strand template strand downstream silencer