HIV Drug Resistance Training Welcome and Introductions 1

HIV Drug Resistance Training Welcome and Introductions 1

Magnet Words § § § Look at the words and phrases on the wall. Go stand by one sign. With others at the same sign, introduce yourselves and discuss: – Why did you choose this sign? – What do you hope to get from this training, related to the words on the sign? 2

Introductions “I am (name), and I am a (role/job) from (where). I am here because (reason for being here/what you hope to learn). ” 3

Purpose of Training § § § Identify ways to measure HIV drug resistance Use HIV DR Assays effectively and efficiently Promote “best practices” in national and regional labs 4

Who the Training is For § National Genotyping Lab Directors – Policy and Advocacy – Lab infrastructure – Develop and manage DR testing programme § Senior Lab Technicians – Technical skills – Responsible for entire testing process 5

Training Outcomes: National Reference Lab Directors Set up a DR lab following quality and biosafety standards (both physical infrastructure and the workflow) § Manage the lab and DR testing program to achieve quality testing/reporting and to support ARV program expansion § Establish QA/QC program for DR testing, including reporting § Select qualified personnel to perform DR testing § 6

Training Outcomes: Senior Lab Technicians § § § § Identify gaps in your own lab and work with the lab directors to achieve standards for DR testing Perform and understand the DR test procedures Monitor the quality of DR testing on a daily basis Manage the entire specimen handling process Ensure timely reporting of test results Manage recordkeeping Manage contamination in PCR and sequencing – how to prevent, diagnose, and resolve Take measures to protect safety of self and others 7

Modules and Agenda § § § § § Overview 1: Introduction to HIV Drug Resistance 2: The WHO Laboratory Network 3: Principles of PCR and HIVDR Sequencing 4: Molecular Lab Set Up and Work Flow 5: Sequencing Procedures 6: Sequencing Interpretation and Analysis 7: Criteria for In-house Assay Validation 8: Dried Blood Spots for HIVDR Genotyping 8

Modules and Agenda § § § § § 9: 10: 11: 12: 13: 14: 15: 16: 17: A Systems Approach To Laboratory Quality Standard Operating Procedures Quality Control And Quality Assurance Data Management Equipment and Supplies Stock Management Specimen Management Biosafety and Waste Management Laboratory Staffing and Training 9

Housekeeping § § § Restrooms Breaks Meals 10