
24ecf5eaf1be550ac8284b15d6ac6bfd.ppt
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Health Effects of Air Pollution March 17, 2010 Presented by David B. Peden, M. D. , M. S. Professor of Pediatrics, Medicine & Microbiology/Immunology Director, UNC Center for Environmental Medicine, Asthma and Lung Biology
Disclosures • CORPORATE • Glaxo. Smith. Kline • Funxional Therapeutics • • • FEDERAL US EPA NHLBI NIEHS NCCAM 2
Sources of Common Environmental Contaminants Which Modify Asthma
National Ambient Air Quality Standards (NAAQS) Pollutant Primary Stds. Averaging Times Carbon Monoxide 9 ppm (10 mg/m 3) 8 -hour(1) 35 ppm (40 mg/m 3) 1 -hour(1) Lead 1. 5 µg/m 3 Quarterly Average Nitrogen Dioxide 0. 053 ppm (100 µg/m 3) Annual (Arithmetic Mean) Particulate Matter (PM 10) Revoked(2) Annual(2) (Arith. Mean) 150 µg/m 3 24 -hour(3) 15. 0 µg/m 3 Annual(4) (Arith. Mean) 35 µg/m 3 24 -hour(5) 0. 075 ppm 8 -hour(6) 0. 12 ppm 1 -hour(7)(Applies only in limited areas) 0. 03 ppm Annual (Arith. Mean) 0. 14 ppm 24 -hour(1) Particulate Matter (PM 2. 5) Ozone Sulfur Oxides
Pollutants and Asthma
Environmental Agents Which Induce Asthma, Allergies and Lung Disease • Probable » Diesel Exhaust » ETS • Possible » O 3 » NO 2 » Other Particulates • Plausible » Low dose endotoxin (Gauderman et al NEJM, 2004, 351: 1057 -1067)
Environmental Agents Which Worsen Asthma and Allergies • • • Ozone SO 2 NO 2 Diesel Exhaust Particulates Environmental Tobacco Smoke • Endotoxin Burnett, RT et al. Am J Epidemiol. 2001 Mar 1; 153(5): 444 -52
Impact of steel mill closing on PM 2. 5 (left, mg/m 3) and Bronchitis admissions (right) in the Utah Valley (Pope, et al) 80 150 70 125 Steel Mill Closed 60 100 50 75 40 30 50 20 25 10 0 0 1985 1986 1987 1988
Endotoxin exposure is a risk factor for asthma: the national survey of endotoxin in United States housing. Am J Respir Crit Care Med. 2005 Dec 1; 172(11): 1371 -7 9
Pollutants shown to enhance recall response to allergen • • • Ozone NO 2/SO 2 Endotoxin Diesel Exhaust ETS Responses include: » » Ig. E PMNs Eosinophils Airway Reactivity 10
Airway Responses of Asthmatics to Ozone
Daily Levels of Ozone (Both 1 -Hour Average and 8 -Hour Average), Particulate Matter of 2. 5 {micro}m or Less (PM 2. 5), and Daily Maximum Temperature, With Daily Prevalence of Respiratory Symptoms for Users of Asthma Maintenance Medication (n = 130) for Southern New England, April 1 through September 30, 2001 Gent, J. F. et al. JAMA 2003; 290: 1859 -1867 Copyright restrictions may apply.
Granulocyte Response to O 3
Effect of O 3 on IL-1 b and IL-6 in Asthma and Normal Volunteers
Effect of O 3 on IL-8 and IL-10 in Asthma and Normal Volunteers
Ozone Enhances Markers of Innate Immunity and Antigen Presentation on Airway Monocytes in Healthy Individuals J Allergy Clin Immunol. 2007 Sep; 120(3): 719 -22
Ozone enhances response to inhaled allergens • Ozone exposure causes increased immediate allergic responses to house dust mite • Ozone causes increased eosinophil (allergy cell) influx to the airway • Experimentally demonstrated at levels as low as 0. 12 ppm
Summary • Epidemiological Studies clearly demonstrate an effect of ambient air ozone in asthma • Ozone causes an immediate decrease in lung function • Ozone induced airway inflammation » PMNs in all populations » Eosinophils-seen in asthmatics • Ozone results in changes in airway cell surface markers which likely modulate immune response • Ozone increases response to allergen
Climate Change, Pollutants and Environmental Allergens
Global temperature land-ocean anomaly (°C), 1880 -2007, defined as the difference from the average temperature of the base period (1951 -1980). The 14 warmest years in the global temperature record have all occurred since 1990. The blue points (annual means) in the box from 1990 -2007 show the warmest years. Adapted from: NASA 2007 20
Predicted increase in ambient O 3 by 2050 resulting from Climate Change Bell et al, Climatic Change (2007) 82: 61– 76 DOI 10. 1007/s 10584 -006 -9166 -7 21
Predicted exceedance of the 1 and 8 hour O 3 standard by 2050 resulting from Climate Change Bell et al, Climatic Change (2007) 82: 61– 76 DOI 10. 1007/s 10584 -006 -9166 -7 22
Singer BD, Ziska LH, Frenz DA, Gebhard DE, Straka JG (2005) Increasing Amb a 1 content in common ragweed (Ambrosia artemisiifolia) pollen as a function of rising atmospheric CO 2 concentration. Functional Plant Biology 32, 667– 670. Increased CO 2 linked to increased Ragweed Pollen and increased Amb a 1 concentration/mg pollen 23
Effect of Urbanization on Ragweed growth Ziska LH et al JACI, 2003 24
Oxidant Stress and Pollutants A unifying concept for the impact of pollutants in asthma
Oxidant stress is a common theme in the action of a wide variety of air pollutants in inflammatory processes. • Pollutants & oxidant stress: » Pollutants themselves • O 3, metals » Products from pollutants • Polyaromatic hydrocarbons & quinones from DEP and ETS » Oxidants from macrophages and granulocytes • Singlet oxygen, H 2 O 2, superoxide Cross, CE et al, American Journal of Respiratory and Critical Care Medicine Vol 166. pp. S 44 -S 50, (2002)
GSTM 1 polymorphisms and lung disease in humans ETS, DEP and O 3
GSTM 1 • NRF 2 nuclear transcription factor • GSTM 1 Null » Associated with increased response to ozone » Associated with increased response to diesel exhaust » Associated with increased response to tobacco smoke
Effect of GSTM 1 null genotype on asthma relative to tobacco smoke exposure • Effect of maternal smoking during pregnancy on asthma in GSTM 1 - children (panel to the left, Gilliland et al, AJRCCM 166: 457 -463, 2002) • GSTM 1 - children exposed to environmental tobacco smoke have a 5. 5 odds ratio risk of current asthma relative to GSTM+ children (Kabesch et al, Thorax 59: 569 2004)
DEP effect on the response to allergen stratified by GSTM 1 genotype Gilliland et al, Lancet. 2004 Jan 10; 363(9403): 119 -25 • Following DEP challenge, GSTM 1 null individuals (vs. GSTM 1+) have increased: » allergen-induced Ig. E (right) » histamine (not shown)
Effect of GSTM 1 genotype and ozone induced decreases in FEF 25 -75 in asthmatics Romieu et al, Thorax 2004; 59: 8 -10 • GSTM 1 null asthmatics have increased asthma symptoms with ozone exposure than do GSTM 1 sufficient persons
Effect of the GSTM 1 null genotype on lung function and inflammatory response to 0. 4 ppm ozone Alexis et al, JACI 2009, available on line 32
Effect of the GSTM 1 null genotype on airway cell function, IL-1 b & IL-8 post ozone challenge GSTM 1 did impact cell function but not IL-1 b or IL-8 Alexis et al, JACI 2009, available on line 33
GSTM 1 and ozone summary • GSTM 1 does not determine lung function repsonse to ozone • GSTM 1 null has a delayed PMN response to ozone • GSTM 1 null vs. sufficient have different cell marker changes in airway monocytes
What to do about pollutantinduced disease Patient and societal interventions
Pollutant Avoidance • • Decrease tobacco use Local Environmental Control Live away from a roadway Societal Changes (decrease traffic, better fuels)
Changes in Traffic Patterns 1996 Atlanta Olympics
Mean Levels of Major Pollutants Before, During, and After the 1996 Summer Olympic Games as a Percentage of the National Ambient Air Quality Standard (NAAQS) Friedman, M. S. et al. JAMA 2001; 285: 897 -905. Copyright restrictions may apply.
Friedman, M. S. et al. JAMA 2001; 285: 897 -905.
Inhaled Steroids Effect on response to pollutants
Inhaled Corticosteroids and Endotoxin • Inhaled corticosteroids » Decreases baseline eosinophilic inflammation » Decreases CD 14 expression on airway monocytes and macrophages » Decreases PMN influx to inhaled endotoxin
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Antioxidants and Asthma Treatment Is this an option?
Vitamin E + C supplementation protects asthmatics from O 3 induced changes in lung function and inflammation children with allergic asthma • Combination ascorbate and alpha tocopherol: » Decrease effect of ambient O 3 exposure on FEF 25 -75 and PEFR » Decrease O 3 related inflammation • Other reports: » Combination therapy decreased lung function response to O 3 challenge in normal volunteers and asthmatics » Ascorbate may protect against exercise-induced asthma Romieu et al, AJRCCM Vol 166. pp. 703 -709, (2002) Sienra-Monge et al, Clin & Exp Immunology 138 (2), 317 -322. (2004) 44
Acknowledgements • • • • Neil Alexis Philip Bromberg John Lay Haibo Zhou Michelle Hernandez Carole Robinette Martha Almond Peg Herbst Aline Kala Lynne Newlin-Clapp Sally Ivins Heather Wells Nolan Sweeney • • Madeline Dillon Jessica Wiser Marlowe Eldridge Robert Roubey Dhaval Patel Robert Devlin David Diaz. Sanchez • Howard Kehrl • Martin Case • Steven Kleeberger 45