
3774f3e1711223ec4e0a5341b1be20c5.ppt
- Количество слайдов: 39
Gastrointestinal Infections: Food for Thought! Prof Eric Bolton Regional HPA Laboratory Manchester Medical Microbiology Partnership Manchester
Topics Included • Background to Food borne Disease • Food Standards Agency (FSA) priorities • HPA contribution to the FSA priorities and new developments • Future activities
Definition of Food Poisoning (Advisory Committee on the Microbiological Safety of Food - 1992) “Any disease of an infectious or toxic nature caused by or thought to be caused by the consumption of food or water. ”
Foodborne Illness Microbiological Food Poisoning • Foodborne Gastrointestinal infections • Gastrointestinal illness associated with toxin producing bacteria • Non Gastrointestinal infections associated with food-borne transmission Intoxication Biological Chemical
Food Poisoning Annual Corrected Notifications England Wales 1984 -2001
Foodborne Disease in England Wales : 1992 - 2000 • 1. 4 million cases in 2000 • >325, 000 general practitioner consultations • 21000 hospital admissions • > 88000 bed days Adak GK, Long SM, O’Brien SJ Trends in Indigenous foodborne disease and deaths, England Wales 1992 – 2000, Gut (In Press)
Food for Thought !! • 1 in 5 members of the population are affected by intestinal disease per year • 9. 4 million people in England suffer • The estimated cost to the Nation is at leastthree quarters of a billion pounds !! - 55% to employers - 36% to the NHS - 8% directly to the case
Food Standards Agency: Priorities Established 1 st April 2000 Strategic Themes: – Food-borne illness – BSE – Chemical Safety of Food – Food Products and Processes – their Licensing and Approval
FSA Strategic Objectives Food-borne Illness (Food Poisoning) • Reduce the incidence of foodborne disease by 20% over the next 5 years • Reduce Salmonella contamination of UK produced retail chicken by at least 50% over the next 4 years
Laboratory Reporting of Selected GI Pathogens in England & Wales.
FSA Strategic Objectives Reducing Foodborne disease: Setting the baseline • Baseline set using the number of UK acquired food poisoning cases in 2000 • Laboratory reports of five main pathogens will be used to monitor success
Wanted Dead or Alive ( Alive is not an option for Food Safety!!) Public Enemy No 1 Public Enemy No 2 Public Enemy No 3 Campylobacter 50773 cases Salmonella 15000 cases E. coli O 157 1035 cases
Wanted Dead or Alive !!! Public Enemy No 4 No 5 Clostridium perfringens 166 cases Listeria monocytogenes 116 cases
Laboratory Reports of Infections with Campylobacter sp. England & Wales (1980 -2002)
Campylobacter Sentinel Surveillance • 1 st May 2000 • Population based sentinel surveillance scheme for campylobacter infection • Generate new hypothesis for infection • Integrating typing and epidemiological data
Campylobacter Sentinel Surveillance, UK • 22 Health Authorities • 12. 5 million population • 15% of laboratory confirmed cases
Food Exposures (a) Eaten once/more than once (b) mg-1 person-1 week-1; National Food Survey, 1999
Distribution of Isolates Among the Clonal Complexes Associated with Human Disease
Salmonellosis in England & Wales.
FSA – Reducing Salmonella in UK retail chicken • Action plan for addressing bio-security, crate washing etc on broiler farms • Survey of current contamination levels
Results of a Public Health Investigation into the use of raw eggs in the UK catering industry 2002
Salmonella from Raw Shell Eggs Used in Catering Country of origin Number of pooled samples positive Salmonella serotypes and S. Enteritidis phage types UK(Lion Quality) 349 0 None found UK (not Lion Quality) 274 2 S. Enteritidis PT 4, PT 6 USA 60 0 None found France 45 0 None found Spain 468 24 S. Enteritidis PT 1, PT 5 c, PT 6 a, PT 6 d, PT 12, PT 13 a, PT 14 b, PT 58 Not known (not Lion Quality) 200 17 S. Enteritidis PT 4, PT 6 a S. Altona, S. Cerro, S. Infantis, S Livingstone, S. Ohio
Verocytotoxin Producing E. coli O 157 ( England & Wales)
Meat Products Associated with Transmission of E. coli O 157 • • • Ground beef products Other red meats Roast beef Dry cured salami Cooked meat products Turkey meat
Dairy Products and other Foods Associated with Transmission of E. coli O 157 • Vegetables • • • Raw milk “Pasteurised milk” Yoghurt Cheese Cream • Salad vegetables • Mayonnaise • Apple cider (USA) • Fruit
Verocytotoxin E. coli O 26 • Isolated from human cases in Germany, England, Australia and other countries • Now more prevalent in Italy than O 157 • Since June 2003 four strains of O 26 have been isolated from cases in Scotland
Average Annual Totals of C. perfringens Food Poisoning
Place of 176 General Outbreaks of Clostridium perfringens: Food Poisoning 1992 -1998 • Residential Institution 46 (26%) • Restaurant/Café 33 (19%) • Hotel/Guest House 15 (8. 5%) • Pub/Bar 14 (8%) • Hospital 13 (7%)
Features of General Outbreaks of Clostridium perfringens: Food Poisoning 1998 -1999 • 66 confirmed incidents • Foods associated with outbreaks are meat and poultry. • Accounted for 95% of outbreaks • Over 45 different serotypes detected in confirmed cases
HPA Activities Contributing to FSA Strategic Objectives: Clostridium perfringens When is a cluster of cases a Foodborne outbreak? • Cluster of cases with relevant onset and syptoms • Demonstration of enterotoxin in faeces • Isolation of a common “type” of C. perfringens from patients and food
PCR for C. perfringens alphatoxin and enterotoxin Enterotoxin fragment Alpha toxin fragment HPA FSML unpublished data
C. perfringens AFLP analysis Mc. Lauchlin et al. Int J Food Microbiol 2000; 56: 21 -28.
C. perfringens food poisoning ‘outbreak’ : Diarrhoea in >25 patients attending a function No. cultures ET in faeces (RPLA) 10 patients 1 patient 6 patients 2 patients 3 patients 2 foods detected not tested NA AFLP type A 7 other types ET gene + ET gene 17 0 12 0 5 3 3 0 4 0 9 0
Annual Totals of Listeriosis Cases in England Wales
Selected Worldwide Outbreaks of Human Listeriosis Country UK Australia New Zealand France Italy USA Sweden France Australia USA France Year 1987 -9 1990 1991 1992 1993 1994 -5 1996 1998 -9 2000 Food Pate Smoked mussels Pork tongue/aspic Pork rillettes Rice salad Chocolate milk Smoked fish Soft cheese Cooked chicken Hot dogs/deli meats Pork rillettes Pork tongue/jelly Serovar 4 b 1/2 a 4 b 4 b 1/2 b 4 b 4 b 1/2 4 b 4 b 4 b
Quandary for the Food Standards Agency • Target is to reduce food poisoning by 20% in 5 years • What proportion of reported Gastrointestinal infections are foodborne?
Future Research Activities • Development and study of nucleic acid archive from the Infectious Intestinal Disease (IID) study • GP based sentinel surveillance of GI infections • Campylobacter case-control study • Role of molecular methods for the investigation of potential non-food sources of Campylobacter jejuni infection
“Does my bum look big in this? ”
3774f3e1711223ec4e0a5341b1be20c5.ppt