FROM IMAGES TO ANSWERS Portfolio de Productos de

FROM IMAGES TO ANSWERS Portfolio de Productos de Media Cybernetics Olympus Latin America Q 2 2006

New Releases for 2006 • Auto. Quant X • In. Vivo/In. Vitro 3 • Image-Pro Plus 6 (Slightly Higher Price but Lots More Stuff) • Scope-Pro 6 (Piezo Support and More…) • Image-Pro Analyzer 6 (Single Seat, 3 -Seat) • Image-Pro Express 6 (Lower Price; More Features) • IPE 3 DS • IPE-MC • Scope-Pro Express 6 (Z and Shutter Control) • Vertical Applications: • IQ-Otolith & IPP-Otolith Macro • EDF Analyzer • IQbase 2

New Releases In. Vivo 3

Top Features in In. Vivo 3 • 1 -Button Data Set Export to Image-Pro Plus 6. 0 • Yes • Finally • Windows XP x 64 Support • Large memory address handling • 64 -bit camera drivers from QImaging and Hamamatsu • Variable Rate Time Lapse Acquisition • Nyquist Calculation for Z-Stack Acquisition • Multi-Camera Support • Support for Up To Four Concurrent Cameras • True Simultaneous Acquisition from Two Monochrome Photometrics or QImaging Cameras • Updated Driver Support for Other Popular Cameras

Top Features in In. Vivo 3 • FRET Acquisition • Live Calibrated Ratio Display

Top Features in In. Vivo 3, part deux • Stage Navigation Upgrades • Cell Chambers and Multi-Well Plate templates

Top Features in In. Vivo 3, part troix • New Microscope Control Dialog Illustrations for Leica Stands • Support for DM 6000, 5000, 4000 & DMI 6000, 5000, 4000

In. Vivo Family Software Series In. Vivo Live Cell Imaging Solution for Calcium Ratios, FRET and Other Dynamic Processes Exp. IO Vital Systems Control and Measurement Image Renamed “In. Vitro” at Lower Price Capture Discontinued

Digital Camera Support • Photometrics Family of Monochrome Cameras (including MAC, but NOT AS FAST! [no circular buffer support]) • QImaging Family of Cameras (including MAC) • Some Hamamatsu Cameras (not including Mac) • Some Stanford Photonics Cameras

In. Vivo Acquisition Camera Control Fully Automated Microscope Control Multi Color Acquisition Time Lapse, Burst Acquisition 3 D, z-series, Burst 3 D with piezo Tiling, Mosaic, Slide and Well Scanning FRET Acquisition Optical Splitter Acquisition

Multi Dimensional Acquisition

In. Vivo Analysis Live Ratio and Ca++ Imaging Intensity Calibration (Including Grynkewicz) Vital Systems Control and Measurement Signals and Systems control Continuous Wavelength Imaging Devices

Live Cell Imaging

Pre-Configured Systems • • Automated Microscopes Digital Cameras Stage Automation Vital Systems

Live Cell Imaging Application Solutions with In. Vivo Bundles

Multi Color Time Lapse Acquisition Live Intensity and Ratio Measurements with Fast Acquisition using In. Vivo Advanced Ratiometric and Intensity-based Tracking in Image-Pro Plus

Time Lapse, Burst Acquisition and Advanced Tracking High Speed Acquisition for Small Region(s) Track Single or Multiple Moving Particles

3 D Acquisition, Deconvolution and Measurement Multicolor Z and Burst Z Acquisition Multi Color Deconvolution 2 D & 3 D Blind Advanced 3 D Analysis and Measurements

Multidimensional Acquisition and Analysis Extended Depth of Field Count Size

Signals and Actions Acquisition and Analysis

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New Releases Image-Pro Plus 6

Top New Features in Image-Pro 6 • Live Image Scaling for Low-Light Applications • Live Data Collection and Charting • XP x 64 Support to Open Larger Image Sets • Open GL 3 D Viewer • Direct Import of QED Files (File, Open) • User-Defined Equation Editor in Data Collector • Dynamically Linked Graphic Display in Data Collector • Embedded I/O Control for Serial and Parallel Devices • ICC Color Profiles

New Open GL 3 D Viewer

More File Support

New Data Collector Tools

New Data Input/Output Tool

New Color Management Tool

Image-Pro 6 Enhancements • Image-Pro Plus 6 Feature Enhancements: • Updated Capture Dialog • Scalable Calibration Markers • User-Specific Menus • “Dark Mode” for Low Light Laboratory Environments • Automatic Measurement of >100, 000 Objects (Memory-Dependent) • Intelligent Macro Editor (not Intelli-Sense, but something much like it ; ) ) • 3 D Convolution Filters • More Extensive File Support (FV 1000, JPEG 2000, etc. ) • Export to Excel or Origin • Generate Movies from Surface Plot • 6 -Month License for IQbase 2 (Optional Permanent License at Extra Cost)

Updated Capture Dialog

New Scalable Calibration

New Menu Editor

New Dark Mode

New 3 D Convolution Filters

Top New Features in Scope-Pro 6 • 3 -Point Predictive Focus for Wavy Samples • Support for Simultaneous Stepper and Piezo Drives (X, Y, and Z) • Calibrated Continuous Zoom Support for Olympus SZ-X • Objective Paracentricity (X, Y) Offsets • Analog/TTL Support for Shutters, Filter Wheels, AOTF’s and Monochromators • Nyquist Calculator for Z-Axis Acquisition • Support for the Olympus ZDC & Nikon Perfect Focus

Image-Pro Family Modules • Automate Motorized Microscopes and Other Acquisition Devices • Sharp. Stack Plus includes: 3 D Blind, 2 D Blind deconvolution algorithms plus No Neighbor, Nearest Neighbor and Inverse Filter algorithms • Back as a Plug-In by Popular Request • A/D & D/A Acquisition & Support for External Devices • Turbo Scan • High Speed Tiling

Camera Support for Image-Pro Family • • • QImaging* (Excellent) Photometrics* (Excellent) Leica DFC* (Excellent) Olympus DP 50 / DP 70 / DP 30 (Excellent) Nikon DXM 1200/F DS 5 (Good) Hamamatsu (Good, 3 rd Party Driver) Cooke PCO (Good, 3 rd Party Driver) Diagnostic Instruments (Fair, 3 rd Party Driver) Lumenera (Fair, 3 rd Party Driver) * Image-Pro 6. X Support

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New Releases IQbase 2

Top New Features of IQbase 2 • IQbase 2 Features: • Capture Module (Optional extra Cost) • Support for Active Directory – Use Windows Identities for log-in and Security Levels • Export Images and Data to Power. Point • Apply Measurements to Archived Images • Compare Images Side-by-Side using Lightbox Tool • Use 3 D Scatterplots to Mine Data in Three Dimensions • Speed Optimized for Analysis, Searching, Opening and Displaying • New Manual Measurement Tools

IQbase 2 Powering Image Informatics • Manages Images and Data • Drives Discovery • Facilitates Collaboration • Easy to Use • Works the Way You Do • Grows with Your Organization (Scalable)

IQstudio Analytical Imaging Development Kit Rapid Development of Image Analysis Applications • Develop Applications Using our Tools • Add Image Analysis to Existing Products • Standardize Image Analysis within the Organization • Distribute Custom Applications with Run. Time Licensing

Deconvolution, 3 D Rendering & Measurement

Deblurring vs Deconvolution Auto-Quant® Algorithms are Used in Sharpstack The No Neighbor & Nearest Neighbor Algorithms are Deblurring Tools (Intensities are Removed) The Inverse Filter Algorithm is a Haze Restoration Tool (Intensities are Re-Assigned) The Optional 2 D Blind and 3 D Blind Tools are Deconvolution Algorithms (Intensities are Re. Assigned) The Sharpstack Algorithms are not Appropriate for Confocal Images Because They Use Widefield PSFs

Deblurring vs Deconvolution

Auto. Quant X

AQI X Advantage • Sequence detection – Multi time point – Multi channel • Multi-Processing, Multi-Threaded • Best in Deconvolution – Only Blind Algorithm Commercially Available – Spherical Aberration Detection and Correction

Sequence Detection • Loads Complex File Sequences • Dynamically Changes to Accommodate Naming of Current File

Multi Time Point • Load 3 D Time Series Data • Visualize, Deconvolve and Analyze as a Single Dataset

Multi Channel • Load any Number of Channels • Change Tints & Thresholds in Real-Time

Multi-Processing, Multi-Threading • Each Operation Runs in its Own Thread • Deconvolve, Visualize a Dataset and Queue Another Dataset at the Same Time • Run Two Deconvolutions Simultaneously • Status Display is a Dashboard for Activity • Takes Advantage of Dual Processor Computers

Blind Deconvolution • Blind Deconvolution Yields the Best Results with the Least Effort • No Need to Acquire or Maintain a Library of Point Spread Functions • Adaptive PSF Changes with the Microscope: – Aberrations, Thermal Expansion, Noise, Alignment, and Specimen are Corrected • Maximum Likelihood Expectation Models Noise Instead of Smoothing it

Hierarchy of Algorithms • 3 D Blind Deconvolution – Premier • 2 D Blind Deconvolution – Live cell • 3 D Non-Blind - Faster for Derived PSFs • 3 D Non-Blind (Gold’s) – Fast but Noisy • 3 D Inverse Filter, Nearest/No Neighbor – Quick and Dirty

Spherical Aberration • Common Problem with RI Mismatches or Thick Samples • Detect Using Iterative MLE or Calibrate • Pre-Bias PSF Improves with Blind Deconvolution • Improves Signal Due to Smearing

Deconvolution of Widefield and Confocal Images Primer on The Growing Role of Deconvolution…

Why Use Deconvolution? To Improve Signal to Noise Ratio

What is Deconvolution? Widefield – Single Channel

Multiple Wavelengths - Widefield

Confocal Microscopy

2 D Blind Deconvolution

2 D Blind Deconvolution – TIRF

When to Use Deconvolution? In Widefield Fluorescent Microscopy – Improve contrast of 2 D and 3 D Images – Improve Resolution in Three Dimensions – Improve 3 D Rendering and Visualization

When to Use Deconvolution? In Confocal Microscopy – Improve Signal to Noise Ratios – Increase Dynamic Range – Improve Image Grayscale Resolution – Increase Axial Resolution

When to Use Deconvolution? Documentation – Images and Projections will Show More Detail and Greater Contrast – Improvements can be Striking

When to Use Deconvolution? Analysis – Deconvolution Retains Quantitative Accuracy – Improves Contrast Facilitating Analysis • • Counting Tracking Area Measurements Intensity Measurements

Why Does This Work? The Microscope is a Well-Understood Optical System The Behavior of Light is Well-Modeled Persistence Why this works (1/5)

The Point Spread of a Microscope • Every Microscope. Sample Combination has a Different Point Spread Function • Every Element in the Optical Path Affects the Point Spread Function: – – Scope Objective Cover slip Sample

The Point Spread of a Microscope Compare a widefield PSF to a Confocal PSF

Looking Forward to Deconvolution Numerical Aperture Emissive Wavelength Spherical Aberration YFP Labeled Motor Neurons (Mouse) Laura Baylor, Harvard University Laboratory of Dr. Jeff Lichtman

Non-Blind Deconvolution Non-Blind deconvolution estimates the original object from the measured PSF and the collected data (2 D or 3 D image).

Blind deconvolution separates the original object and the point spread from the collected data (2 D or 3 D image).

3 D User Interface

3 D User Tools • Pointer to Toggle between Measurements & Volume Display • Pan Tool to Adjust 3 D Volume Position • Home Position • Set New Home Position • Change Perspective (View) • Display Box Around Volume • Change View Y, Z or Z, X or Y, X • Use Control Click for LR or UD, Click for UD, Alt Click to Toggle Between Pan and Pointer

3 D User Options

3 D Rendering with 3 D Filters

3 D Volume Measurements

3 D Volume Measurements Over Time

3 D Branch / End Point Measurements

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