FROM IMAGES TO ANSWERS Image Pro Plus 5

FROM IMAGES TO ANSWERS Image Pro Plus 5. 1 Introduction

Image-Pro Plus 5. 1 More Answers from your Images!

Image Pro Plus 5. 1 New Features • Improved Memory Manager – Load Image Sets Larger than Available RAM – Breaks Through the “Hard Wall” at 2 GB for Windows® Applications* with Image Stacks – Useful with Today’s Larger Digital Image Stacks – Works Seamlessly and Silently in the Background * Cannot open a single image larger than 2 GB

Image Pro Plus 5. 1 New Features • Macro Player – Load and run the various macros within IPP – Demonstrating a range of applications

Image Pro Plus 5. 1 New Features • Stitching and Tiling – Align and stitch images together – Save or load a set of calculated alignments to be used on Fluorescence images

Image Pro Plus 5. 1 New Features • Alignment correction – align individual images, or images in a sequence, for translation, rotation, or scaling – Useful for images taken over time, images with some camera movement, aligning channels in multiple images from a beam splitter, or correcting for offset angles through a stereo microscope Z-stack.

Image Pro Plus 5. 1 New Features • Improved Automatic Tracking – Correlation Tracking on Active AOI – Full Correlation or Phase Correlation Options for Tracking under Different Image Conditions – More than 50 Morphological Measurements available including Intensity Tracking – Smart Search Radius Detects Object Movement from Image – New Coherence Filter Adjusts for Neighbor Vectors – 1 -Button Static or Dynamic Intensity Tracking

Image Pro Plus 5. 1 New Features Toolbar Extensions • Use Existing Menu Icons • Create New Icons • Include Self-Contained Macros with no External Parameters

IQbase (Now Included with Image Pro Plus) Powering Image Informatics • Manages Images and Data • Drives Discovery • Facilitates Collaboration • Easy to Use • Works the Way You Do • Grows with Your Organization (Scalable)

MACROS in IPP (Gradient Paint & Create VRML Surface Plot) • Gradient painting macro • Generate Test strips Create Surface plot VRML (Virtual Reality Modeling Language ) VRML Viewing plug-in required (http: //www. parallelgraphics. com/products/cortona/download/)

MACROS in IPP (Gradient Paint & Create VRML Surface Plot)

MACROS in IPP (Gradient Paint & Create VRML Surface Plot)

MACROS in IPP (FRET) • FRET Calculations

Image-Pro Family Software Series • Perfect for Basic Imaging or Capture Stations • More Advanced than Image. Pro Express, Includes Support for Plug-Ins • The Ultimate Imaging Package, Includes Support for Macros and Plug-Ins

Image-Pro Family Bundles • Image-Pro Advanced Microscopy Suite This complete solution for high-level microscopy analysis and acquisition combines Image-Pro Plus 5. 1 with powerful tools for multi-dimensional microscope control, image deconvolution, and 3 D rendering. • Image-Pro Multi-Dimensional Acquisition Ideal for automated microscopy work, this package combines Image-Pro Plus 5. 1 with tools for multidimensional microscope control and acquisition. • Image-Pro 3 D Suite Ideal for deconvolution and interactive control and analysis of volume stacks, this package combines Image-Pro Plus 5. 1 with deconvolution and rendering tools. • Image-Pro Microscope Control The Image-Pro Microscope Control (MC) package combines Image-Pro Plus Version 5. 1 with tools to control and program all major automated microscopes.

Image-Pro Family Bundles • Image-Pro 3 D Discovery Perfect for confocal microscopy, the Image-Pro 3 D Discovery package combines Image-Pro Discovery 5. 1 with tools to provide full interaction and analysis of all 3 D volumes. It includes major confocal microscope support. • Image-Pro Analyzer Ideal for networked users who want advanced analysis tools but do not need image capture functionality at each work station. Image-Pro Analyzer offers all of the powerful analysis features of Image-Pro Plus 5. 1, but does not include support for acquisition. 10 Unit Network 5 Unit Network

Image-Pro Advanced Microscopy Suite • Image-Pro 5. 1 Advanced Microscopy Suite • Includes: • Image Pro Plus 5. 1 • 3 D Constructor • Sharpstack (Sharp. Stack Plus available as an Option) • AFA • Scope-Pro • IQbase • AMS Benefits • Lower Customer Prices • Avoids Version Conflicts • One CD Loads All Programs • Upgradeable from Earlier Image Pro Versions

Image-Pro Family Bundles • Image-Pro Multi-Dimensional Acquisition • Includes: • Image Pro Plus v. 5. 1 • Advanced Fluorescence Acquisition 5. 0 • Scope-Pro 5. 0 • IQbase • MDA Benefits • Lower Customer Prices • Avoids Version Conflicts • One CD Loads All Programs • Upgradeable from Earlier Image Pro Versions

Image-Pro Family Bundles • Image-Pro Microscope Control • Includes: • Image Pro Plus 5. 1 • Scope-Pro 5. 0 • IQbase • MC Benefits • Lower Customer Prices • Avoids Version Conflicts • One CD Loads All Programs • Upgradeable from Earlier Image Pro Versions

Image-Pro Family Bundles • Image-Pro 3 D Suite • Includes: • Image Pro Plus v. 5. 1 • Advanced Fluorescence Acquisition 5. 0 • Scope-Pro 5. 0 • IQbase • MDA Benefits • Lower Customer Prices • Avoids Version Conflicts • One CD Loads All Programs • Upgradeable from Earlier Image Pro Versions

Image-Pro Family Bundles • Image-Pro 3 D Discovery • Includes: • Image Pro Discovery 5. 1 • 3 D Constructor 5. 0 • IQbase • MC Benefits • Lower Customer Prices • Upgradeable from Earlier Image Pro Versions • Avoids Version Conflicts • One CD Loads All Programs • Standalone Version Ideal for Confocal Applications

Image-Pro Family Bundles • Image-Pro Analyzer • Includes: • Image Pro Plus v. 5. 1 Network Analysis Only (No Capture) • IQbase Analyzer Benefits • Lower Customer Prices (Network 5 or 10 Seats)

Image Pro Multi-Dimensional Acquisition

Image Pro Multi-Dimensional Acquisition

Image Pro Microscope Control • Software control of stages, filter wheels, shutters, z-motors, microscopes • Ludl, Marzhauzer, Prior, ASI and Objective Imaging • Leica, Olympus, Zeiss, Nikon • Sutter, Physik

Image Pro 3 D Suite • 3 D reconstruction, visualization and volumetric and spatial measurements • Major Features – Free Rotation of Image Stacks – Extensive Animation & Display Tools – Orthogonal & Oblique Sectioning – Numerous Spatial & Volumetric Measurements – Time Resolved Tracking of Objects

Image Pro 3 D Suite Spatial Measurements

Image Pro 3 D Suite Automatic Volumetric Measurements

Image Pro 3 D Suite Automatic Volumetric Measurements Over Time 4 D Tracking of objects!!

Image Pro 3 D Suite Neuron Analyzing (Neuron Spines) !!

Image Pro 3 D Suite Includes: No Neighbor, Nearest Neighbor Haze Removal and Inverse Filter Haze Restoration Algorithms These Algorithms are not Appropriate for Confocal Images An Option is Available which Includes 2 D Blind and 3 D Blind Deconvolution Algorithms

Image-Pro Family Modules • Automate Motorized Microscopes or Devices (available separately until 12/2004) • Sharp. Stack Plus includes: 3 D Blind, 2 D Blind deconvolution algorithms as well as No Neighbor, Nearest Neighbor and Inverse Filter algorithms • Color-Pro Color Matching System for Camera, monitor and printer

Color-Pro Module • Consistent Color • Better Quantitative Results • ICC calibration tools for camera, screen, printer and projector

Evolution VF - cooled & un cooled - mono & color • Features – Designed for high resolution brightfield scientific and industrial applications. – A progressive scan interline CCD sensor gives a resolution of 1. 3 million pixels in a 12 -bit digital output. (Sony 205 Sensor) – High-speed low noise electronics provide linear digital data – Live Frame rates of up to 110 fps with reduced region of interest and binning. – The IEEE 1394 Fire. Wire™ digital interface allows ease of use

Micropublisher 3. 3 Integrated Color Camera Kit • High resolution 3. 3 megapixel color camera • Features • Fully integrated with your choice of Image-Pro software • Fire. Wire (IEEE 1394) C-mount camera • Max 25 fps at 640 x 480 (30 -bit) Real Time Video (RTV) • Option for cooling • No interpolation or Pixel shifting • Laptop kit

Evolution MP™ Integrated Color Camera Kit • High resolution 5. 1 megapixel Color Camera (Sony 282 Sensor) • Features – Fully integrated with your choice of Image-Pro software – Fire. Wire (IEEE 1394) C-mount camera – Max 25 fps at 640 x 480 (30 -bit) Real Time Video (RTV) – Option for cooling – No interpolation or Pixel shifting – Laptop kit

Evolution QEi™ Integrated Camera Kit Ideal for demanding Lowlight and fluorescence imaging High Quantum Efficiency Camera Features – Fully integrated with your choice of Image-Pro software – Fire. Wire (IEEE 1394) C-mount camera – Fast Fire. Wire Live 15 fps at 1360 x 1036(12 -bit)

Evolution Camera Series GFP Quantum Efficiency (%) 80 TRITC CY 5 70 With IR Filter (Std) Without IR Filter (Removed) DAPI 60 50 IR-DIC 40 30 20 10 0 300 400 500 600 700 800 Wavelength (nm) 900 1000 1100

Evolution™ Camera Series Media Cybernetics Evolution MP 5. 1 Media Cybernetics Evolution VF Media Cybernetics Evolution QEi Resolution 2560 X 1920 1392 X 1040 1360 X 1036 CCD Type ICX 282 (Color/Mono) ICX 205 (Color/Mono) Sensor Grade Pixel Size Full Well Read Noise Dark Current Optical Interface Depth Cooling Max live size Live fps Binning (Max) Gain Control ROI (User defined) Zero Defect 3. 4 μm × 3. 4 μm Zero Defect 4. 65 μm × 4. 65 μm 8, 600 e- 10, 000 e- 18, 000 e-, 22, 000@2 x 2 9. 8 e- 12 e- 8 e- 2. 3 e-/p/s (cooled) 1 e-/p/s (cooled) 0. 15 e-/p/s (cooled) 2/3” C Mount ½” C Mount 2/3” C Mount 36 -bit 12 -bit(mono) 36 -bit 10° C below ambient 25° C below ambient 2560 X 1920 1392 X 1040 1360 X 1036 3 fps (full) 30 fps@640 X 480 (RTV) 4 X 4 15 fps (full) @ 12 -bit 0 – 10 X Yes RGB Filter Option Warranty Zero Defect 6. 45 μm × 6. 45 μm 8 X 8 0 – 15 X Yes 2 yrs 12 -bit(mono) 8 X 8 0 – 35 X Yes 2 yrs

Applications Evolution QEi™ §Bright field, Phase- Contrast, & Darkfield Microscopy §Fluoresecence Microscopy §Live- Cell Imaging §Pathology, Histology, & Cytology §Green Fluorescent Protein (GFP) Applications §FISH §Ca++ Ratio, TIRFM, Analysis §Motility & Motion Analysis §DNA Analysis §Metallurgical Microscopy §Semiconductor Inspection §Manufacturing Quality Control §Failure Analysis §Forensic Analysis §Spinning Disk Confocal system

Applications Evolution VF™ §Brightfield, Phase- Contrast, & Darkfield Microscopy §Fluoresecence Microscopy §Live- Cell Imaging §Pathology, Histology, & Cytology §Motility & Motion Analysis §DNA Analysis §Metallurgical Microscopy §Semiconductor Inspection §Failure Analysis §Forensic Analysis

Applications Evolution MP 5. 1™ §Brightfield, Phase- Contrast, & Darkfield Microscopy §Fluoresecence Microscopy §Pathology, Histology, & Cytology §Haematology §Document Imaging §Still Image Animation

Cool. SNAP HQ Mono QEi with IR Filter QEi without IR Filter Orca ER 150 RT SE 6 with IR Filter RT SE 6 without IR Filter GFP 80 Quantum Efficiency (%) Sensicam QE LOW LIGHT Sensicam QE Standard Mode TRITC DAPI 70 CY 5 60 50 IR-DIC 40 30 20 10 0 300 400 500 600 700 800 Wavelength (nm) 900 1000 1100

Pro-Series MV-Pro & MV-Lite Capture Kits • Board, Cable & Driver Included • Works with Image Pro Express, Discovery or Plus • Works with Most Video Cameras • Specialized Cables also Available • New version available soon

Turboscan Fast Mosaic Image Acquisition For Image-Pro® Plus • Scanning and acquisition at camera frame rates Support for various analog and digital cameras, including Media Cybernetics' Evolution VF and QImaging's QICAM cameras High-quality image tiling • Frame-rate shading correction • Fast and accurate relocation • Continuous focus tracking using predictive focus • Compatible with most 3 rd party motorized stage and focus drives • Intelligent memory management supports mosaic sizes limited only by available disk space • Plug-in capture driver for use within Image-Pro® Plus 5. 1

X, Y or Z Stage Kit • X, Y-axis Stage and/or Z-axis motor • Controller box • Use With Image Pro 5. 1 MC or MDA, AMS • All necessary cables

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