Fluorescence Cameras -Dr James Milnes -Live Cell Imaging

Fluorescence Cameras -Dr James Milnes -Live Cell Imaging – Stem Cell Research -Portland Place, London, 24 June 2009 www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 1

Overview • Company Profile • Product Description • Current Applications • Fluorescence Applications – Gated Cameras – Photon Counting Cameras • Summary www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 2

Company Profile • Specialist manufacturers of vacuum based detectors and camera systems for photon detection. Photek manufacture Image Intensifiers, PMTs, Streak Tubes, open faced detectors and a range of associated electronics and camera systems • Located in St Leonards-on-Sea – South East England • Established September 1991 www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 3

Company Profile • 38 Employees – 10 sales/administrative – 7 R&D – 21 production/test • 5 employees educated to PHD level • Further 10 employees educated to degree level • Expected £ 3 million turnover in 2009 • ISO 9001: 2000 certified www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 4

Detector Products • MCP image intensifier • Ultra fast MCP photomultipliers • Streak tubes • Magnetic focus image intensifiers • De-magnifying gen 1 image intensifiers • Vacuum compatible detectors www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 5

Camera Products • Photon counting • Nanosecond ICCD • Intensified CCD • Fluorescence • X-ray • Particle imaging www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 6

Electronic Products • HT power supplies • Nanosecond gating control • Laser pulse generator • Preamplifier • Image intensifier cooling www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 7

Applications • Early warning Missile systems • Wafer silicon inspection • Astronomy including orbital space science experiments • Ultra-high speed cameras • Nuclear & high energy physics research • Life-science research • Industrial processing • Security www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 8

Space Science Projects • ACE Advanced Composition Explorer • XMM UV Optical Monitor • MSX UVISI Detector • GUVI Global Ultraviolet Imager • SSUSI Special Sensor Ultra-violet Spectrographic Imager • UVIT Ultra-Violet Imaging Telescope www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 9

UV Optical Monitor • Photon Imaging 170 nm to 550 nm region • Aligned with X-Ray Telescope • Provides Simultaneous Visible, UV and X-ray Images www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 10

Technology Applications in Fluorescence • Light Amplification – Intensified Camera – Photon Counting Camera • Time resolution – Gated Intensified Camera ~ 3 ns – Photon Counting Camera ~ 100 ps www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 11

Gated Intensified Camera • Time resolution obtained by turning detector ON and OFF very quickly, equivalent to mechanical shutter • Gating is non–mechanical : All electronic • Electronic gate unit required to switch between +50 V and – 200 V very quickly • Aim for adjustable gating down to single ns www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 12

Gating of MCP based Detectors ON OFF Cathode - 200 V + 50 V MCP IN 0 V 0 V MCP OUT + 1000 V + 5000 V Screen / Anode Cathode www. photek. co. uk MCP IN MCP OUT Live Cell Imaging – Stem Cell Research 24 June 2009 Screen or Anode page 13

Gating Results - Method Gate Trigger ON OFF 40 ps Laser Pulse Generator & Independent Delay Controller • Laser is stepped through gate window • Effectively frames response to 40 ps “delta” input • Allows characterisation of detector response to gate www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 14

Gating Results – Image Intensifiers • 18 mm diameter Image Intensifier • 200 ps per frame • Gating Window is moved through the region of interest to reconstruct the observed Fluorescence in time www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 15

Photon Counting Camera • More gain than Intensified Camera • Detects single photon events • Each photon can be resolved in time and space • Time Consuming: Need 10 Fluorescent cycles to count 1 photon www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 16

Photon Counting: Time Resolution • Each photon is imaged in (x, y) and tagged in time t. • A histogram of photon events is generated • Photon Counting is usually a serial process www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 17

In Development: Parallel Photon Counting • Hi-Content Project: – Space & Particle physics technology into Life Science • Collaboration with CERN, GCI, Leicester & Manchester Uni • Multiple anodes connected to NINO ASIC devices • Aim for 20 ps resolution, 10 Mcounts/sec per channel www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 18

Hi-Content Applications • The ‘omics: particularly Proteomics – The study of protein interactions in vivo • Time resolved spectroscopies – Fluorescence lifetime imaging – Fluorescence correlation spectroscopy • Plus others e. g. Raman, polarization anisotropy, dielectric, etc. • Other applications: – Optical tomography, etc. www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 19

Summary • Photek is a specialist manufacturer of vacuum based detectors & cameras to industry & universities • We can offer gated camera systems with 3 ns time resolution • Our photon counting cameras can currently achieve 100 ps resolution in serial mode • A parallel readout of 10 Mcounts / second per channel with 20 ps resolution is in development www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 20

Thank you for listening www. photek. co. uk Live Cell Imaging – Stem Cell Research 24 June 2009 page 21