Extracted from presentation given by Robin Todd at 2007 Ent Soc. America meeting ICR, Inc. Robin Todd, ICR, Inc.
Clean. Zone • • • A non-chemical method that works Elevated ozone levels – 100 ppm + Ozone Generator Confined spaces Used for mold elimination Bed bugs a new target Robin Todd, ICR, Inc.
Test • • Clean. Zone Systems LLC. Lisle, IL Conducted study in an empty home Showed efficacy Asked ICR for independent confirmatory test Robin Todd, ICR, Inc.
Safety • EPA’s acceptable level: 0. 08 ppm for 8 hours (0. 07 - 0. 075 ppm proposed) • Clean. Zone generates 100 ppm + • Test run in green house – safety • Ozone levels monitored • Clean. Zone staff attended test and ran generator Robin Todd, ICR, Inc.
Test details • • Test chamber built 8 x 18 x 7 ft tall (1008 ft 2) Plastic sheeting over PVC piping Sand snakes hold down plastic Robin Todd, ICR, Inc.
Bed bug containment & Replication • 10 containers with screened lids • 10 containers covered with cloth • 10 adult bed bugs per container Robin Todd, ICR, Inc.
Test Chamber Robin Todd, ICR, Inc.
Control Chamber • 4 x 6 x 7 ft high chamber (168 ft 2) • In a different building – no elevated ozone Robin Todd, ICR, Inc.
Other species tested • • German cockroaches Confused flour beetles Pharaoh ants American house dust mites Robin Todd, ICR, Inc.
Containers of insects Robin Todd, ICR, Inc.
Observations during exposure • Ozone levels observed hourly • One container of each species removed hourly - Knockdown checked = monitors • Self-sealing port – avoid ozone loss Robin Todd, ICR, Inc.
Results • • • Ozone in test chamber 118. 3 ppm (mean) Generator run for 22. 7 hours Peak (150. 7 ppm) at 9 hours Still air – underestimated levels Ozone levels in control chamber: 0 – 0. 1 ppm Robin Todd, ICR, Inc.
Ozone levels Robin Todd, ICR, Inc.
Results • 100% knockdown in monitor containers for all species except ants Robin Todd, ICR, Inc.
Results • Test Chamber - 100% mortality +24 hours after removal from chamber • 4% mortality in control Robin Todd, ICR, Inc.
Hourly Knockdown Robin Todd, ICR, Inc.
Dead bed bugs after treatment no longer clinging to filter paper Robin Todd, ICR, Inc.
Other Species • Treatment killed 100% of roaches and dust mites • 86% (open) 98% (covered) of beetles • 40% (open) 83% (covered) of ants – Ants in covered nest containers – because sheltered from ozone? Robin Todd, ICR, Inc.
Acknowledgement • John Sharpe, formerly with ICR, conducted this test. He did a great job. Robin Todd, ICR, Inc.
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