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Enhanced Myeloid Progenitor Cell Cycling and Apoptosis in Mice Lacking the Chemokine Receptor, CCR Enhanced Myeloid Progenitor Cell Cycling and Apoptosis in Mice Lacking the Chemokine Receptor, CCR 2 by Suzanna Reid, Alec Ritchie, Landin Boring, Jennifa Gosling, Scott Cooper, Giao Hangoc, Israel F. Charo, and Hal E. Broxmeyer Blood Volume 93(5): 1524 -1533 March 1, 1999 © 1999 by American Society of Hematology

Representative dot plot of murine BM cells stained with fluorescently conjugated antibodies that recognize Representative dot plot of murine BM cells stained with fluorescently conjugated antibodies that recognize c-kit (y axis) and the following lineage markers: B 220, CD 3, or CD 11 b (x axis) (A). Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Numbers of c-kit+lin− cells in total BM. c-kit+lin− cells (from the upper left-hand quadrant Numbers of c-kit+lin− cells in total BM. c-kit+lin− cells (from the upper left-hand quadrant of Fig 1 A) were gated analyzed for their percentage in the total population of nucleated BM cells and the results are expressed as the number of cells per femur. Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Average percentages of apoptotic c-kit+/lin− cells in BM. c-kit+lin− cells were gated separately from Average percentages of apoptotic c-kit+/lin− cells in BM. c-kit+lin− cells were gated separately from the remainder of the BM population and analyzed for MC 540 fluorescence intensity (MC 540 bright cells represent the apoptotic fraction of the gated populati. . . Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Representative dot plot that distinguishes c-kitbrightlin− from c-kitdimlin− cells. Suzanna Reid et al. Blood Representative dot plot that distinguishes c-kitbrightlin− from c-kitdimlin− cells. Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Cycling rates of myeloid progenitors in c-kit+lin− BM. The cycling rates of progenitors were Cycling rates of myeloid progenitors in c-kit+lin− BM. The cycling rates of progenitors were determined by tritiated thymidine kill assay of sorted c-kit+lin− BM cells. Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology

Effects of delayed addition of growth factors on survival of CFU-GEMM, CFU-GM, and BFU-E. Effects of delayed addition of growth factors on survival of CFU-GEMM, CFU-GM, and BFU-E. Suzanna Reid et al. Blood 1999; 93: 1524 -1533 © 1999 by American Society of Hematology