DNA Test Validation Update Dick Quaas Cornell University

DNA Test Validation Update Dick Quaas Cornell University 31 Oct. 2006 NBCEC Brown Bagger R. L. Quaas

Quantitative Trait DNA Tests Available Bovigen Gene. STAR®. . . Report # Stars & GEPD 1. Gene. STAR® Tenderness (3 SNP) 2. Gene. STAR® Quality Grade (4 SNP) 3. Gene. STAR® Feed Efficiency (4 SNP) IGENITY ® Profile. . . 1 -10 score 1. IGENITY® Tenderness (3 SNP) 2. IGENITY® Carcass Composition (5 -10 SNP) MMI. . . MGV 1. Tru-Marbling™ (128 SNP) 2. Tru-Tenderness™ (11 SNP) 31 Oct. 2006 NBCEC Brown Bagger R. L. Quaas

Bovigen Gene. STAR® http: //www. bovigen. com/products 1. Gene. STAR® Quality Grade Marbling Score NBCEC 2. Gene. STAR® Tenderness 14 d WBSF NBCEC 3. Gene. STAR® Feed Efficiency Intake (NFI=RFI? ) not feed efficiency No request for validation 31 Oct. 2006 NBCEC Brown Bagger R. L. Quaas

IGENITY® Profile http: //www. igenity. com/ IGENITY® Carcass Composition Ribeye area Quality grade (MS) Hot carcass weight Yield grade Fat thickness IGENITY® Tenderness 14 d WBSF NBCEC Feed intake/RFI panel being analyzed Analyses in progress 31 Oct. 2006 NBCEC Brown Bagger R. L. Quaas

MMI http: //www. mmigenomics. com/products 1. html Tru-Marbling™ & Tru-Tenderness™ MMI reported AAA CMP data analysis @ NCBA (see MMI website) NBCEC subsequently analyzed these data 31 Oct. 2006 NBCEC Brown Bagger R. L. Quaas

NBCEC AAA CMP Analyses (not validation) Estimates b p SE 1: MS=CG+e Variance Components Residual Variance Reduction Sire Residual Sire "h 2" 7422 2: MS=CG+b x MGV+e 0. 47 0. 14 1. 3 E-03 7239 3: MS=CG+b x MGV+Sire+e 0. 45 0. 15 3. 1 E-03 6761 915 2. 0% 5. 1% 0. 48 4: MS=CG+Sire+e 6901 964 0. 49 1: WBSF=CG+e 2. 5% 2. 13 2: WBSF=CG+b x MGV+e 0. 50 0. 09 2. 6 E-08 1. 97 3: WBSF=CG+b x MGV+Sire+e 0. 50 0. 09 7. 6 E-08 1. 87 0. 21 7. 2% 19. 7% 0. 41 2. 02 0. 27 0. 47 4: WBSF=CG+Sire+e 31 Oct. 2006 NBCEC Brown Bagger 7. 8% R. L. Quaas

NBCEC AAA CMP Analyses (not validation) Estimates b p SE 1: MS=CG+e Variance Components Residual Variance Reduction Sire Residual Sire "h 2" 7422 2: MS=CG+b x MGV+e 0. 47 0. 14 1. 3 E-03 7239 3: MS=CG+b x MGV+Sire+e 0. 45 0. 15 3. 1 E-03 6761 915 2. 0% 5. 1% 0. 48 4: MS=CG+Sire+e 6901 964 0. 49 1: WBSF=CG+e 2. 5% 2. 13 2: WBSF=CG+b x MGV+e 0. 50 0. 09 2. 6 E-08 1. 97 3: WBSF=CG+b x MGV+Sire+e 0. 50 0. 09 7. 6 E-08 1. 87 0. 21 7. 2% 19. 7% 0. 41 2. 02 0. 27 0. 47 4: WBSF=CG+Sire+e 31 Oct. 2006 NBCEC Brown Bagger 7. 8% R. L. Quaas

NBCEC AAA CMP Analyses (not validation) Estimates b p SE 1: MS=CG+e Variance Components Residual Variance Reduction Sire Residual Sire "h 2" 7422 2: MS=CG+b x MGV+e 0. 47 0. 14 1. 3 E-03 7239 3: MS=CG+b x MGV+Sire+e 0. 45 0. 15 3. 1 E-03 6761 915 2. 0% 5. 1% 0. 48 4: MS=CG+Sire+e 6901 964 0. 49 1: WBSF=CG+e 2. 5% 2. 13 2: WBSF=CG+b x MGV+e 0. 50 0. 09 2. 6 E-08 1. 97 3: WBSF=CG+b x MGV+Sire+e 0. 50 0. 09 7. 6 E-08 1. 87 0. 21 7. 2% 19. 7% 0. 41 2. 02 0. 27 0. 47 4: WBSF=CG+Sire+e 31 Oct. 2006 NBCEC Brown Bagger 7. 8% R. L. Quaas

MMI http: //www. mmigenomics. com/products 1. html Tru-Marbling™ & Tru-Tenderness™ MMI analyzed AAA CMP data (see MMI website) NBCEC also analyzed these data Not independent validation Awaiting genotypes from MMI of ISU Angus for validation 31 Oct. 2006 NBCEC Brown Bagger R. L. Quaas

NBCEC Web Site http: //www. nbcec. org/nbcec/ Click on validation tab for most recent results. 31 Oct. 2006 NBCEC Brown Bagger R. L. Quaas