DNA-Photonic crystal Fluorescence Spectra Vitaliy Boyko 1, Galina Dovbeshko 1, O. Fesenko 1, V. Gorelik 2, V. Moiseyenko 3 1. Institute of Physics, pr Nauki 46, Kyiv, Ukraine, phone: +380 (44) 5259851; E-mail: vb@iop. kiev. ua 2. P. N. Lebedev Physical Institute of the Russian Academy of Sciences, Leninsky Prospect, 53, Moscow 119991, Russia 3. Dnipropetrovsk National University, Prospect Gagarina 72, Dnipropetrovsk 49050, Ukraine Introduction Globular photonic crystals (PC) is of great interest for optics, optoelectronics and sensor application [1] due to their potential in control of propagation and emission of light. When the light travels within a periodic crystal structure consisting of the elements with spacing of order of wavelength of the light, it could be diffracted by PC structure leading to formation of stop zones. A photonic band zone could be formed if the stop zones will overlap on all directions of the light propagation and for all polarizations. Stop zone formation influences the optical property both PC and molecules inserted in the cavities of PC [2]. Materials and Methods Synthetic opals were produced in Zelenograd (Russia) and Dnepropetrovsk (Ukraine). Nanodispersive silica globules (with particle size near 250 nm in diameter) were synthesized by the method of Stober et. al [3]. We used the DNA water solution (2 mg/ml) after centrifugation during 0. 5 hour with UZDN-A (Sumy), denaturation at 100°C and rapid cooling up to 0°C. Structure of synthetic opal Microstructure (SEM images in SEI mode) of synthetic opal Images of surface of the synthetic opal visualized with the confocal microscope (wavelength of excitation 365 nm). Bragg reflectance spectra Images of synthetic opal (optical microscope) Fluorescence spectra Bragg reflectance spectra for different points on the surface of the photonic crystal. Fluorescence of photonic crystal at Т = 77 K (λexc = 270 nm). Bragg reflectance spectra at different incidence angles. Fluorescence of DNA –Photonic crystal at Т = 77 K (λexc = 270 nm). Conclusions Fluorescence of DNA has been registered at Т = 77 K (λexc = 270 nm) synthetic opal and “DNA-photonic crystal” complex with increased yields due to the essential increase of radiation field caused by the slow diffuse motion of incident photons into sample volume and as a result of surface enhanced conditions inside opal pores. Acknowledgments We thank Ukrainian-Russian project № 71 -02 -10 “Radiation of 3 -D PC under optical and electric excitation” for its financial support [1] V. S. Gorelik Optics of globular photonic crystals // Quantum electronics, Vol. 37, No. 5, pp. 409 (2007). [2] V. N. Moiseyenko, M. P. Dergachov, V. G. Shvachich, A. V. Yevchik. // Ukr. J. Phys. Opt. Vol. 10, No. 4, pp. 201 (2009). [3] W. Stober, A. Fink, E. Bohn. Controlled growth of monodisperse silica spheres in the micron size range // J. Coll. Interf. Sci. Vol. 26, pp. 62 -69 (1968).
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