detect and identify Bioanalytik Benefits Over 25 years of experience in Imaging Night. OWL
detect and identify Bioanalytik In 1989 BERTHOLD TECHNOLOGIES developed the first low level light imager In 1993 BERTHOLD stated in its brochure to do non-invasive bioluminescent in-vivo-imaging In 1996 Night. OWL became introduced
detect and identify Night. OWL benefits (1) Interchangeable Cameras NC 100 >-80°C cooled backlit CCD camera 1 M pixel; 1024 x 1024 pixel; 1: 1 13 x 13 µm pixel size dr 1 : 50. 000 (best for in-vivo imaging) NC 320 >-60°C cooled frontlit CCD camera 3. 2 M pixel; 2154 x 1640 pixel; 1, 3: 1 6, 8 x 6, 8 µm pixel size dr 1 : 8. 000 (best for fluorescence) No PC-board anymore. Connecting via Ethernet! Bioanalytik
detect and identify Bioanalytik Night. OWL benefits (1) Sensitive Cameras 10 luminescent cells in total are detectable in a microplate well.
detect and identify Night. OWL benefits (1) Sensitive Cameras 1000 luminescent cells in total or 20 cells per mm² subcutaneously in a mouse are easy detectable. Detection limit is 150 – 250 cells in a cluster. Bioanalytik
Bioanalytik detect and identify Night. OWL benefits (2) Win. Light – Powerful scientific software for Night. OWL (1) Easy setting of sample size and height Easy setting of acquisition time from 30 ms up to hours. Analysis is possible via Line plot Threshold 3 D-plot ROI manually Edit functions Color overlay Pseudo colors Multicolor overlay
detect and identify Bioanalytik Night. OWL benefits (2) Win. Light – Powerful scientific software for Night. OWL (2) Everything is defined by the user Flatfield correction Height correction in each camera position Cosmic suppression Background substraction Arithmetric functions Geometrical Analysis (area, length etc. ) Quantitative Analysis Same camera position Same exposure time Same threshold Sequence measurements and Analysis Macro function for full automated image analysis
detect and identify Bioanalytik Night. OWL features (3) User defined pixel binning Pixel are combined (“binned”) to superpixel indepentently. Per readout process one readout noise is generated, independent whether a single pixel or a superpixel is read out. Pixel binning increases the s/n ratio: one readout noise and a big signal Loss of spatial resolution.
detect and identify Night. OWL benefits (4) Moving camera and Working distance 3, 5 cm 75 cm Focus (cm) Resolution (µm) 3. 5 50 75 320 Bioanalytik
detect and identify Night. OWL benefits (5) Power sockets and connectors inside for individual set-ups like Extra lamps Cooler or heater ? Bioanalytik
detect and identify Night. OWL benefits (6) Flange option with the flange option additional in- and out-lets are possible For For gas hot water fibre optics electric cables Bioanalytik
Bioanalytik detect and identify Night. OWL benefits (7) Macro table Distance is important
detect and identify Bioanalytik Night. OWL benefits (7) ds. Red fluorescence Gooseneck Macro table
detect and identify Night. OWL benefits (8) C-mount adapter Via C-mount adapter the Night. OWL cameras can be easily attached to each microscope with C-mount port. BERTHOLD offers a specific microscope option, so that only 2 thumb screws has to be unscrewed. Bioanalytik
Bioanalytik detect and identify Night. OWL benefits (9) Fluorescence Sophisticated lamp control Constant lamp beam via Photodiode control Lamp factor can be set to calibrate the system.
detect and identify Night. OWL benefits (9) Flexible fluorescence epi-illumination 3 modells of illumination are available Ringlight epi illumination Dual-line epi illumination Gooseneck spot illumination Filter slide for emission 90 W tungsten light source Filters available from 200 - 1100 nm Bioanalytik
Bioanalytik detect and identify Night. OWL benefits (9) GFP fluorescence and NIR-fluorescence Ex. : 680 nm Em. : 720 nm
detect and identify Night. OWL benefits (9) Full range of fluorescence trans-illumination Transilluminators with 20 x 20 cm illuminated area 8 lamps with wavelenghts of 256 nm 312 nm 365 nm Blue White + converters Bioanalytik
detect and identify Night. OWL benefits (10) Gas anaesthesia system (1) Via the flange option the anesthesia gas flow is directed in and out. Inside up to 5 rodents can be anesthesized. Bioanalytik
Bioanalytik detect and identify Night. OWL benefits (10) Gas anaesthesia system (2) Vaporizer Gas flow meters Flange option with gas in- and outlet Knock-out box
detect and identify Bioanalytik Night. OWL benefits (11) Orthogonal 3 D-option With such a mirror system images from top, left and right can be obtained. Measuring the lenght, width and depth of the light source, volume calculation can be done with r 2 = 0, 89.
detect and identify Night. OWL benefits (11) Orthogonal 3 D-option In the overlay images you can use the line-plot function to measure length, width (from top-image) and height (from the right image) to calculate the volume according to the simple formula L x W x H / 2. Bioanalytik
Bioanalytik detect and identify Night. OWL benefits (12) To perform experiments with different imaging technologies, a carrier for MRI and PET was developed by medres. MACU = Multimodal Animal Carrier Unit
detect and identify Bioanalytik Thanks for Over 25 years Your attention! experience in Imaging
Скачать презентацию detect and identify Bioanalytik Benefits Over 25 years
c40ce2184366002410d816165058954f.ppt