Chapter 16 Hsueh-Fen Juan Oct 30 2012

Chapter 16 Hsueh-Fen Juan Oct 30, 2012

16. 1 Cloning DNA

Cut and Paste

Animation: Base-pairing of DNA fragments

Animation: Restriction enzymes

Making Recombinant DNA

DNA Cloning

Cloning Vectors

DNA Cloning

Animation: Formation of recombinant DNA

c. DNA Cloning

c. DNA Cloning

16. 2 From Haystacks to Needles

DNA Libraries

Animation: How to make c. DNA

Probes

Nucleic Acid Hybridization using a Radioactive Probe

Animation: Use of a radioactive probe

Big-Time Amplification: PCR

PCR

PCR

Animation: Polymerase chain reaction (PCR)

16. 3 DNA Sequencing

DNA Sequencing

Tagged Dideoxynucleotides

DNA Sequencing

Animation: Automated DNA sequencing

Next Generation Sequencing (NGS)

16. 4 DNA Fingerprinting

DNA Fingerprints

Creating DNA Fingerprints

DNA Fingerprints: A Forensics Case

Animation: DNA fingerprinting

16. 5 Studying Genomes

The Human Genome Project

Sequencing the Human Genome

Genomics

DNA Chips

DNA Chips and Gene Expression

Principle of c. DNA microarrays

Principle of c. DNA microarrays

Principle of oligonucleotide arrays

Construction of Oligonucleotide Arrays

Construction of Oligonucleotide Arrays

16. 6 Genetic Engineering

GMOs

Transgenic Organisms

16. 7 Designer Plants

The Ti Plasmid

Ti Plasmid Transfer

Animation: Gene transfer using a Ti plasmid

Animation: Transferring genes into plants

Genetically Engineered Plants

Some Genetically Modified Plants

16. 8 Biotech Barnyards

Of Mice and Men

Examples of Transgenic Animals

Some Genetically Modified Animals

Knockout Cells and Organ Factories

16. 9 Safety Issues

Into the Unknown

16. 10 Modified Humans?

Gene Therapy

Getting Better

Getting Worse

Getting Perfect

ABC video: Glow-in-the-Dark Pigs

Synthetic Biology