Ca. MKII-δ Isoform Regulation of Neointima Formation After Vascular Injury by Suzanne J. House, and Harold A. Singer Arterioscler Thromb Vasc Biol Volume 28(3): 441 -447 March 1, 2008 Copyright © American Heart Association, Inc. All rights reserved.
Figure 1. A, Immunoblots of uninjured right (R), injured left media (L), and neointima (LNeo) with antibodies recognizing all (pan-CKII), specific (CKIIδ 2, CKIIγ) Ca. MKII isoforms, or β-actin. Suzanne J. House, and Harold A. Singer Arterioscler Thromb Vasc Biol. 2008; 28: 441 -447 Copyright © American Heart Association, Inc. All rights reserved.
Figure 2. A, Immunoperoxidase staining of GFP in arteries 3 days after injury and transduction with adenovirus coexpressing sh. Luciferase (sh. Luc). Suzanne J. House, and Harold A. Singer Arterioscler Thromb Vasc Biol. 2008; 28: 441 -447 Copyright © American Heart Association, Inc. All rights reserved.
Figure 3. A and C, Ca. MKII isoform and PCNA expression in R and L medial smooth muscle 3 and 7 days after injury and transduction with shδ 2 or sh. Luc adenoviruses coexpressing GFP. Quantification of medial PCNA signals 3 (B) and 7 (D) days after injury. Suzanne J. House, and Harold A. Singer Arterioscler Thromb Vasc Biol. 2008; 28: 441 -447 Copyright © American Heart Association, Inc. All rights reserved.
Figure 4. A, Ca. MKII isoform and PCNA expression in R and L adventitial specimens 3 days after injury and transduction with shδ 2 or sh. Luc adenoviruses coexpressing GFP. Quantification of relative Ca. MKII isoform protein (B) and PCNA expression (C) from samples in (A). Suzanne J. House, and Harold A. Singer Arterioscler Thromb Vasc Biol. 2008; 28: 441 -447 Copyright © American Heart Association, Inc. All rights reserved.
Figure 5. A, Immunostaining of nuclear histone-H 1 (red), endothelial VCAM-1 (brown), or neointimal Ca. MKIIδ 2 (brown) on luminal surfaces of control, 1 -day, right and 3 -day injured left carotid arteries. Suzanne J. House, and Harold A. Singer Arterioscler Thromb Vasc Biol. 2008; 28: 441 -447 Copyright © American Heart Association, Inc. All rights reserved.
Figure 6. A, Ca. MKII isoform expression in uninjured R and L medial specimens 14 days after injury. Suzanne J. House, and Harold A. Singer Arterioscler Thromb Vasc Biol. 2008; 28: 441 -447 Copyright © American Heart Association, Inc. All rights reserved.
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