C-Reactive Protein-Induced In Vitro Vasorelaxation Is an Artefact

C-Reactive Protein-Induced In Vitro Vasorelaxation Is an Artefact Caused by the Presence of Sodium Azide in Commercial Preparations by Carmen W. van den Berg, Karolina E. Taylor, and Derek Lang Arterioscler Thromb Vasc Biol Volume 24(10): e 168 -e 171 October 1, 2004 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. Relaxation responses of endothelial-intact rabbit aortic rings to CRP. A, Relaxation responses to in-house recombinant CRP (▴), CRP purified from human ascites (▾), and commercial recombinant CRP (Calbiochem) (▪) in phenylephrine (1 μmol/L) precontracted endothelium-intact rings. ***P<0. 001 cf. in-house recombinant and ascites derived CRP. B, Relaxation responses to commercial recombinant CRP (Calbiochem) (▪), dialyzed commercial CRP (□), and sodium azide (1. 92× 10− 8 to 1. 92× 10− 5 M, concentrations equivalent to that found in the commercial CRP, ♦) in phenylephrine (1 μmol/L) precontracted endothelium-intact rings. ***P<0. 001 cf. ascites derived CRP. Carmen W. van den Berg et al. Arterioscler Thromb Vasc Biol. 2004; 24: e 168 -e 171 Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. Relaxation responses of endothelial-denuded rabbit aortic rings to CRP. Responses to commercial recombinant CRP (Calbiochem) (▪) and dialyzed commercial CRP (□) in phenylephrine (1 μmol/L) precontracted endothelium-denuded rings. ***P<0. 001 cf. ascitesderived CRP. Carmen W. van den Berg et al. Arterioscler Thromb Vasc Biol. 2004; 24: e 168 -e 171 Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. Binding of CRP to C 1 q and PC. A, Binding of C 1 q to CRP coated on enzyme-linked immunosorbent assay plates: in-house recombinant CRP (▴), ascites CRP (▾), Calbiochem CRP (▪), and dialyzed Calbiochem CRP (□). Carmen W. van den Berg et al. Arterioscler Thromb Vasc Biol. 2004; 24: e 168 -e 171 Copyright © American Heart Association, Inc. All rights reserved.