C 2 V-200 a new Nessi compatible fast

C 2 V-200: a new Nessi compatible fast Micro GC IFPAC, Baltimore 2008 C 2 V BV, Enschede, The Netherlands IFPAC 2008 1

Job Elders, Ph. D § § § CEO of C 2 V Ex GM for Alcatel Optronics NL with fiduciary responsibility Founder of TMP (Total Micro Products) Member of Nano. Ned Industrial Advisory Board & Invited referee of Dutch Nanotechnology programme (€ 245 M) Invited member of advisory board of Point One (€ 500 M Philips, ASML, Economic Affairs program) Member of program committee IOP precision technology Advisor of Technology Accelerator of University of Twente Co-chair of the international MANCEF MEMS Roadmap (http: //www. mancef. org) Board member of the international magazine “Micro Nano”, Executive board member and founder of MANCEF Member of the Management of Technology Advisory Board of the Anderson school of Management, University of New Mexico Author of about 75 patents, technical and business papers Ph. D in Physical Chemistry, University of Amsterdam § § Board member of Dosimetrix LLC Board member of foundation “Kokopelli” § § § § IFPAC 2008 2

Key historical milestones 2007 1991 2000 2002 BBV founded BBV and TMP acquired by Kymata Management buy C 2 V successfully pre-out of AONL to launches its Micro Gas form C 2 V Chromatograph 2005 Customer Shipments (Eng. Prototypes) 1995 2003 2006 TMP founded BBV Software launched IFPAC 2008 2001 Kymata acquired by Alcatel. BBV and TMP integrated to form Alcatel Optronics NL C 2 V re-aligned to market demand Product introduction Beta site testing OEM product evaluation 3

C 2 V’s fast micro GC: enables Nessi compatibility • 2000 -2002: Ne. SSI Generation I – mechanical, passive components are on a SP 76 substrate • 2002 -2004: Ne. SSI Generation II – “electrified” passive & active components are on a SP 76 substrate – integral heating and temperature control of the substrate – all electrical components are integrated with SAM – sensors and actuators communicate via sensor bus – smart (self-diagnostic) sample systems • 2005+: Ne. SSI Generation III – C 2 V-200: first high quality SP 76 compatible micro. GC – C 2 V-200: micro analyzer adds value to NESSI platform • 2007: Tipping point - First large scale commercial application: – Exxon together with Siemens implements Nessi compatible sample conditioning for SPT (Singapore Parallel Train – chemical plant in Singapore) IFPAC 2008 4

Fast micro GC vs. conventional GC Technical Features / Benefits Conventional GC (Macro drawbacks) Fast micro GC Cartridge (Micro advantages) • • • Life-time Reliability Reproducibility Calibration (s) Quantitation (ppm) Consumables Limited life-time (6 months) IFPAC 2008 System loading x 10< IC derived manufacturing IC-based tolerances, QA/QC Highly controlled operating parameters (temperature, flow, etc. ) • Highly stable system performance • Longer life-time, lower flow rates, Extended life-time (2 -5 years) smaller • faster • better • for less costs 5

Fast micro GC versus conventional GC Technical Features / Benefits Conventional GC (Macro drawbacks) Fast micro GC Cartridge (Micro advantages) • System pre-programmed • Requires skilled engineers • Requires trained service-men • Exchange of cartridge by laymen • Requires significant Cap. Ex • Does not require significant investment Cap. Ex • Enables innovative revenue model, from Cap. Ex to Op. Ex budgets Requires expert know how Complicates sales process IFPAC 2008 Simplifies usage Simplifies sales process smaller • faster • better • for less costs 6

C 2 V-200 micro GC C 2 V’s unique MEMS micro fluidics integration platform enables a world-class handheld chip-based GC instrument. → → → Fast analysis: 10 -30 seconds Handheld sized Easy exchangeable GC channel cartridges Low power system (< 15 W typical) Low costs (decrease per increasing volume) → → → Excellent separation: N=10’ 000 -25’ 000 Operating up to 180 o. C Fast T ramping: 100 o. C / minute ppm sensitivity with TCD Less carrier/reference gas (operational 1 ml/min. ) Diagnostics on chip: flow/pressure IFPAC 2008 smaller • faster • better • for less costs 7

C 2 V’s micro GC technology platform Chip technology • Using micro technology to create low cost GC components (valves, injector, detector, column, flow system, heating, flow diagnostics) with low internal volume and low thermal mass Integration of chips • Using a fluidic multi chip module for integration of these chip components in a fluidic system with low dead volume connections Cartridge • The fluidic system module is assembled on an exchangeable cartridge that contains the core GC functionality IFPAC 2008 smaller • faster • better • for less costs 8

GC Components: chips technology Micro Injector: ® ® ® Micro TCD Detector: Channel array chip Membrane valves Pneumatic actuation ® ® ppm sensitivity ms response Silicon columns: ® ® Fast heating Integration on-chip Capillary columns: ® ® IFPAC 2008 smaller • faster • better • for less costs Commercial available Opt. geometry 9

Fast GC Cartridge C 2 V fast GC easy exchangeable cartridge (2” x 3. 5” x 0. 6”) contains: ® ® Injector and sample loop TCD detector Column Heating (2 zones) C 2 V-200 micro GC module (3” x 4. 5” x 1. 2”) contains: ® ® ® IFPAC 2008 Easy exchangeable cartridge Gas tight cartridge package Read-out & control electronics Pneumatics hardware Connectors (gas & data & power) smaller • faster • better • for less costs 10

Fast GC Cartridges Cartridge Column Type ID [mm] Df [µm] Phase Ratio 100% dimethylpolysiloxane 0. 18 1 45. 0 GCC 1010 PLOT-U 0. 25 8 7. 81 GCC 1020 WAS (PEG) 0. 15 0. 25 150 GCC 1030 Molesieve 0. 32 30 2. 67 GCC 1040 Carbon. PLOT 0. 32 3 26. 7 Typical rentention time Typical efficiency 10’ 000 -15’ 000 plates Typical flow rate IFPAC 2008 15 seconds 1 -10 ml/minute smaller • faster • better • for less costs 11

Fast GC Cartridges GCC 1000 separation is almost entirely based on boiling points making this cartridge suitable for a wide range of applications. Typical components: alcohols, esters, flavors and aromas, free fatty acids, glycols, hydrocarbons, ketones, organic acids, oxygenates, PAHs, pesticides, polymers, steroids, solvents, sulfur compounds. GCC 1010 elutes polar compounds as perfectly symmetrical peaks, allowing them to be analyzed together with light hydrocarbons or permanent gases. Typical components: halogenated compounds, hydrocarbons C 1 -C 6, H 2 S, ketones, oxygenated hydrocarbons, permanent gases/solvents. GCC 1020 provides optimal selectivity for many aqueous soluble compounds. Typical components: BTEX, methanol, alcohols, aromatic hydrocarbons, esters, FAME, flavors and aromas, free fatty acids, glycols, halogenated compounds, ketones, organic acids and solvents. GCC 1030 (molecular-sieve coated capillary column) is especially valuable when separating permanent gases. Typical gases separated with the GCC 1030 include: He, H 2, O 2, CO, Ne, HD, N 2, NO, Ar, D 2, CH 4, Kr. HT, X 3, DT, CD 4, Rn, T 2. GCC 1040 separates N 2, O 2, CO 2 and C 1 -C 2 hydrocarbons. Typical gases separated by the GCC 1040 include: He, Xe, CO, Ne, CH 4, CO 2, O 2/Ar, C 2 H 6, N 2, C 2 H 4, Kr and C 2 H 2 IFPAC 2008 smaller • faster • better • for less costs 12

C 2 V’s fast micro GC: data management • C 2 V platform accommodates: – – Fieldbus Profibus Modbus Wireless • And interfaces with: – RS 232 – USB IFPAC 2008 smaller • faster • better • for less costs 13

Natural gas analysis (up to C 5) VB 1 column: → 12 seconds @ 50 C → High separation quality → C 2 -C 5, neo-pentane visible Poraplot column: → 18 sec @ 80 C → High separation quality → N 2, CH 4, CO 2, C 2 H 6, C 3 H 8 • Injection time 10 msec • Column 50 o. C, chip 100 o. C • Pactuation = 3 bar • PHE=PNG = 1. 5 bar • column: VB 1 • column: 5 m/0. 18 mm/1 mm IFPAC 2008 smaller • faster • better • for less costs 14

Natural gas analysis: C 3 -C 6 IFPAC 2008 smaller • faster • better • for less costs 15

Natural gas: Temperature Program Run IFPAC 2008 smaller • faster • better • for less costs 16

BTEX headspace measurement → Very fast separation < 20 seconds (60 o. C) → Excellent separation quality → Zero peak tailing → Headspace injection 5 m CP-Sil-8, 60 o. C, 2 bar, 200 ms injection time IFPAC 2008 smaller • faster • better • for less costs 17

Reproducibility test: > 100, 000 runs to date Showing results of 5, 000 consecutive runs Demonstrates: stability and reliability: – Flow control – Temperature control – Injection repeatability IFPAC 2008 smaller • faster • better • for less costs 18

Reproducibility Retention time results: 5, 000 consecutive runs IFPAC 2008 smaller • faster • better • for less costs 19

Reproducibility Peak height results: 5, 000 consecutive runs IFPAC 2008 smaller • faster • better • for less costs 20

C 2 V-200: fast Micro GC Concept to Volume B. V. C 2 V For further information or to test a fast, miniature GC for evaluation please contact us at mini. GC@c 2 v. nl Dr. J. Elders, CEO phone: +31 534 889 www. c 2 v. nl Colosseum 20; 7521 PT Enschede The Netherlands IFPAC 2008 21