AQUABLEND AVIAN Trial Information By KIM JINTAE Asia

AQUABLEND AVIAN Trial Information By KIM, JINTAE (Asia Manager)

Introduction v To help customer’s understanding, we were gathering some recent commercial trials data from Korea. v In Korea, we have recommended to use AQUABLEND AVIAN during day 1 -3 in drinking water or anytime at veterinarian’s discretion. v At present, we have many customers who are using AQUABLEND AVIAN in broilers, layers, ducks and breeders. v At 35 -36 days old, AQUABLEND AVIAN-treated birds have shown around 150 g more in body weight in comparison with non-treated birds.

Feeding trial with Broiler integrator 1 v Place: Halim’s commercial farm. 16 OCT 2008 – 14 NOV 2008. v Birds: Ross 64000 birds. v Trial design • Control: 32, 000 birds were fed with normal diets. • Treatment: 32, 000 birds were fed with normal diets + AQUABEND AVIAN (50 g/10, 000 birds) from day 1 -day 3 * Halim is the most big broiler integrator in Korea.

Effect on Livability, % [Korea, 2008]

Effect on Avg. Body Weight, kg * AQB group showed higher ABW by 240 g [Korea, 2008]

Feeding trial with Broiler integrator 2 v Place: Halim’s commercial farm. 23 SEP 2008 – 31 OCT 2008. v Birds: Cobb 39, 000 birds. v Remarks; All groups were treated in the following program. • • • Day 4, 5, 6: antibiotics treatment at 0. 2 -0. 3% Day 7: vaccination for ND Day 13: vaccination for Gumboro virus Day 15, 16: anticoccidial treatment Day 20, 21: ammoxicilin treatment v Trial design • Control: 20, 100 birds were fed with normal diets. • Treated: 18, 900 birds were fed with normal diets + AQUABEND AVIAN (50 g/10, 000 birds) from day 1 -day 3

Effect on Livability, %

Effect on Avg. Body Weight, kg + 0. 12 kg

Effect on FCR

Feeding trial in a commercial broiler farm v v Place: private commercial farm. AUG, 2008. Birds: Ross 49, 000 birds. Period: for 32 days trial Remarks; All groups were treated in the following program. – – – – Day 1, 2, 3: IBD vaccination by spray Day 4, 5, 6: antibiotics treatment; enrofloxacin Day 7, 8: multi-vit. and mineral supplements Day 9: IBD vaccination Day 10: multi-vit. and mineral supplements Day 13: ND vaccination Day 14, 15, 16: anticoccidial + antibiotics treatment Day 22 - 28: multi-vit. and mineral supplements v Trial design – Control (House A): 20, 000 birds were fed with normal diets + organic acids in drinking water + probiotics – Treated (House B): 29, 000 birds were fed with normal diets + AQUABEND AVIAN (50 g/10, 000 birds) from day 1 -day 3

Effects on broiler performances Results; Before AQB, in the same poultry house (B), its ABW(kg) was just 1. 35~1. 4 kg at day 30. Note; According to final results, it seems there were no differences between groups. However, actually, there were significant increment in the body weight in AQUABLEND AVIAN treated group. So far, from the house of treated group, it has always shown poor growth performances in this poultry house compare to other house (control group). Farmer suspected certain existing diseases/pathogens could not be eliminated by any disinfectants. Most of birds from that house showed 1. 35 -1. 4 kg of body weight before at day 30. At the same period, 1. 5 kg of body weight in the house (B) was treated with AQUABLEND AVIAN was tremendous increment in comparison with previous records.

Increasing of Body Weight + 0. 125 kg * In the same house (B), there were a significant increment in body weight. * In addition, there was no Gumboro disease after treating AQB. But, it was happen frequently in this house before. It has been repeated in other trials. If there were serious & continuous Gumboro outbreak, AQB could reduce it’s frequency and intensity.

Growth promoting effect of AQUABLEND AVIAN ü Following data were collected from end-users in Korea market. ü 50 g/10, 000 birds dosage during day 1, 2, 3 was recommended. ü Only 3 farms were used AQUABLEND AVIAN at day 1 and 2. the graph will be showed in blue color. ü At the beginning, those farms had conducted simple feeding trial compare to non-treated group. ü All of farms had different rearing programs for vaccination, antibiotics, nutritional supplements etc.

Differences in weight gain after treating AQUABLEND AVIAN: Simple feeding trials at farms. 250 200 differences in weight, g 150 100 50 0 at day 36 at day 38 at day 35 at day 39 at day 33 at day 35 at day 37 at day 36 at day 35 * From all of them, we found AQB improved growth performance. Especially, in body weight, treated group showed more weight gain of 140 g (average).

Univ. Trial • Place: Iran, 2009 • Purpose: Evaluation of Intestinal Microflora: – Tow separated groups were selected including 20 broilers in each one. – The first group was considered as a Control Group (not treated group). – In second group, broilers received Aqua-Blend Avian product during 3 first days of cycle (based on recommended concentration by manufacturer) in their drinking water. – Eight birds from each group were post-mortem examined and evaluated intestinal content to detect type and number of intestinal microflora at first day ( before the beginning of assay ) and days 3, 7, 14 and 21.

No. of Lactobacilli bacteria/gram of Intestinal Microflora on day 3 rd 5. 33 x 108 cfu/g 1. 13 x 108 cfu/g Control Aquablend Avian

Lactobacilli bacteria/gram of Intestinal Microflora on day 7, 14 and 21 st • Total beneficial bacteria counts were 3 to 5 times.

Coliform bacteria/gram Coliform bacteria of Intestinal Microflora on day 21 st 12. 8 x 103 cfu/g 5. 7 x 103 cfu/g

Coccidium Counts on day 7 th and 14 th * Coccidium count: n x 103 cfu/gram

• Purpose: Evaluation of Production Performance – One hundred of one day broilers were divided into 2 equal groups (each group consisting of 5 sub-groups including 10 birds), we considered first group as a Control Group. The second group received Aqua-Blend Avian product in its drinking water during 3 first days of cycle (based on recommended concentration by manufacturer). – The indexes of production such as increasing of body weight, food intake, food conversion ratio (FCR) on days of 14, 21, 28 and 42 in addition to efficiency rate of production at the end of experiment (on day 42 nd) were measured.

Improved Growth Performance of Aquablend Avian Group Body Weight Compare to control group Day 21 st----------- 6. 5% Day 28 th----------- 4. 9% Day 42 nd----------- 4. 5% FCR: AQB group was improved by 1. 5%, compare to control group.

SUMMARY v For several years, AQUABLEND AVIAN has given a good protection against intestinal pathogens (Salmonella, E. coli, Clostridium etc. ) and improvement in growth performances for poultry over the world. v AQUABLEND AVIAN can provide a natural and safe protection and more weight gain in poultry. v AQUABLEND AVIAN is applying during first 3 days. v AQUABLEND AVIAN can make birds exert their genetic potential through promoting intestinal healthy environment in the early of birds’ life. v AQUABLEND AVIAN can reduce Gumboro disease outbreak rate.

THANK YOU VERY MUCH! AGRANCO CORP. USA If you have any questions, please send me an e-mail: Kim Jin Tae Southeast Asia Manager. agranco. asia@gmail. com