Advances in Forensic DNA Developing Molecular Applications for

Advances in Forensic DNA: Developing Molecular Applications for Saving Lives Department of Biomolecular Engineering Seminar Jack Baskin School of Engineering Steven Lee Professor and Director of Forensic Science San Jose State University steven. lee@sjsu. edu 408 -924 -2948 05 February 2009

Top 10 ways you can tell you’re a forensic DNA scientist 10. You have your kid’s DNA types vs. their pictures on your wall. 9. When your kids were born, you got an extra newborn footstick smpl. 8. When your kids get injured you are more interested in collecting the blood than dressing the cut. 7. You have a backlog of fingernails, hair, and teeth from every member of your willing lineage awaiting testing. 6. You want to open a paternity service for the Jerry Springer show. 5. When you stay in hotels you bring a portable UV light so you can avoid unsanitary latent stains, and then you collect them. 4. You know forensic DNA takes longer than a CSI commercial break. 1. You see stains where no one else can. 2. You spend your day looking at dirty underwear. 1. Your license plate reads “ OJ DID IT!” * Modified from Butler, J. 2005 ACS Meeting presentation

Outline I. Brief Intro to the SJSU Forensic Science Program Overview Grants and Acknowledgements II. Forensic DNA Historical Perspective Applications of Forensic DNA Overview of Forensic DNA typing and recent SJSU research applications Selected Research Projects a. Development and Evaluation of a New DNA Extraction Kit for Degraded Skeletal Remains Nat’l Res. Inst. Police Sci. , Dept Legal Med, Shinshu University School of Medicine , Hitachi Software Engineering Co. , Ltd. , Mirai. Bio, SJSU b. Optimizing Shipping, Storage & Processing of Forensic Samples AFDIL, Biomatrica, CSU LA, FBI, FSS, ICMP, NIST, SDSO, SJSU, RCMP, U Carleton, UNTHS c. Enhancing Amplification of Low Quantity and Low Quality Samples using a new PCR enhancer: PCRBoost AFDIL, Biomatrica, NIST, SJSU, SCCCL

SJSU Forensic Science Program Spearheaded by the late Dean: Dr. Inger Sagatun-Edwards • BS Forensic Science- Conc. Biology or Chemistry Interdisciplinary collaboration: Bio, Chem, JS, Santa Clara County Crime Laboratory • 6 years old: Launched 10/2003 • Justice Studies Department home: 75 yrs old, > 900 majors, 3000 alumni. • 10 fold increase in FS majors (20 -->260) • >20 FS research presentations • Over 75% grads hired, interning or in grad school: – 6 crime labs (Alameda, Kern County, OPD, San Mateo, SCCCL, SFPD) – 2 biotech/pharma (Gilead, Bayer) – 2 med exam techs (SCCME) – 3 investigators (CA DOI Fraud, DMV, CA DOJ) – 3 in grad school: SJSU MS, UC Davis MS, UCSC Ph. D

Active SJSU Forensic Science Grants • Howard Hughes Medical Institute Grant – 2008 -2011: In collaboration with Biology: $1, 300, 000. 00 – 4 year research support forensic DNA undergraduate research • NSF REU RUMBA : http: //www. biology. sjsu. edu/rumba/index. html – 2007 -2010: Co-PI with Dr. Julio Soto, Biology – Collaboration with Santa Clara County Crime Laboratory – 10 week summer research program in molecular biology- $204, 000. 00 • CAC Reed Mc. Glaughlin: Forensic Science Scholarships – 2006 -2009: $13, 000. 00, $5000. 00 for 2009 – CAC A. Reed and V Mc. Glaughlin Endowment Scholarships – Support student research: 3 awards available in 2009 • CSUPERB Joint Venture Grant – 2008 -2009: $35, 000. 00 – Support for undergraduate biochem DNA storage research • Justice Studies Scholarships – Over $10, 000. 00 annually in scholarships for JS majors – New scholarship to cover the entire senior year

New Santa Clara County Crime Laboratory • Dedication on Wednesday, September 3, 2008 • 250 West Hedding Street 10 minutes from SJSU • Four-stories, totaling 90, 072 sq. ft. • Full Service: Toxicology, Vehicle Examination, Computer Forensics, Trace Evidence, Questioned Documents, Latent Prints, Firearms, Full Length Firing Range, Forensic Biology, Evidence Property Unit, Photo Unit, Administration Offices, Training Classrooms and Support Spaces.

Selected Student Research Projects • Evaluation of Purification Columns for DNA Extraction- Marissa Meinenger: In collaboration with Brooke Barloween- SCCCL- Presented at the 2008 ISHI • Recovery of DNA profiles with Amplification Enhancers- Linda Le; Presented at the 2008 ISHI • Optimizing DNA Storage- Kimberly Clabaugh; Presented at the 2008 AAFS meeting • Optimization of fluorescent detection of biological stains- Clarissa Trogdon- New HHMI student • Non-DNA Projects: – Latent print residue analysis for determining the relative age of the fingerprint: Jamie Lunkley – FS Chem major with Dr. Frank Rossi of Chemistry – QD: Analysis of fraud resistant inks: Wendy Farell – JS MS student – Impact of Random Match Probability on Case outcome- Milani

Acknowledgements • Consortium Members: AFDIL, CSU LA, FBI, FSS, ICMP, NIST, SDSO, SJSU, RCMP, U Carleton, UNTHS • Grant Support: – NIJ Grant Support to Roberts and Muller – NSF-REU grant # DBI-0647160 to Dr. J. Soto, Dr. S. Lee and Dr. C. Ouverney – CSUPERB Grant to S. Lee • Commercial Support and Products: – – Bio. Matrica- Dr. Peggy Faix, Bret Light Miraibio: Tateo Nagai, Takashi Shimizu Qiagen: Qia. Safe Applied Biosytems: 7500, Quantifiler, Identifiler

Acknowledgement This research was supported by NSF-REU grant # DBI 0647160. I would like to thank my mentors, Mrs. Lynne Burley, Dr. Ian Fitch and Dr. Steven Lee for their unwavering assistance and guidance during this research. I also want to thank Dr. Elizabeth Mc. Gee (Department of Biological Sciences, San Jose State University, CA) for her encouragement and support. My appreciation also goes to the scientists at Santa Clara County Crime Laboratory for their support, Dr. Soto and other RUMBA mentors for their feedback and other participants in the RUMBA 2007 program. Finally, I would like to thank my family for their understanding and support during this research program.