2008 Applera Corporation and MDS Inc MRMPilot

© 2008 Applera Corporation and MDS Inc. MRMPilot™ Software

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics • Create robust peptide MRM assays without protein standards • Choose the best MRM using the MIDAS™ workflow • Import proteins directly from discovery data • Built-in fragmentation rules for PTM’s, m. TRAQ™ reagents, and more © 2008 Applera Corporation and MDS Inc. MRMPilot™ Software

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics MRMtouch productivity. A: assays offer create a selectivity for sensitivity for quantitatively and use One. By how do youthe highest. MIDAS™ Workflow of the QTRAP® System detecting Q: But combining the uniquerobust MRMand Targeted Proteomics without thethe of peptides… MRMPilot™ software. automated peptide standards? © 2008 Applera Corporation and MDS Inc. MRMPilot™ Software

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics Data from discovery experiments can be imported manually or automatically into MRMPilot™ software… © 2008 Applera Corporation and MDS Inc. MRMPilot™ Software

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics The method is run by LC MRM on thecan be chosen andof interest forbuilt. optimization. best theoretical MRM transitions biological sample the method MRM © 2008 Applera Corporation and MDS Inc. MRMPilot™ Software

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics The unique MIDAS™ workflow can be enabled to obtain high-sensitivity, full-scan MS/MS for peptide confirmation with a simple tick in a box. © 2008 Applera Corporation and MDS Inc. MRMPilot™ Software

AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics One actual productivity. the MIDAS can included in peptide ID confirmation and touch MRM from choosing the most sensitive MRM confidence detecting peptides The MRM table makes. MRM assays workflow provideswithtransition Onlyoptimized, robust “Optimized”™ backnow MRMPilot™ Software for to quantify the Full scan MS/MS data are importedwill be into be usedthe final assay. foranalysis and can be MRM labeled as and proteins interferences… optimization. protein identify used to easy. in hundreds of biological samples. © 2008 Applera Corporation and MDS Inc. MRMPilot™ Software