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The Complete EBAT (Evidential Breath Alcohol Test) Colorado Department of Public Health and Environment The Complete EBAT (Evidential Breath Alcohol Test) Colorado Department of Public Health and Environment Laboratory Services Division EBAT Program Rev. 3. 09 9 -1

CONTENT 1. 2. 3. 4. 5. 6. 7. 8. Appendix 2 A Review Functions CONTENT 1. 2. 3. 4. 5. 6. 7. 8. Appendix 2 A Review Functions Key Stand By Mode Warm Up Period Diagnostic Tests Ready Mode Test Sequence (ACABAIA 2 ABACA) EBAT Print Out Rev. 3. 09 9 -2

APPENDIX 2 A Refer to 5 CCR 1005 -2 page 25. Rev. 3. 09 APPENDIX 2 A Refer to 5 CCR 1005 -2 page 25. Rev. 3. 09 9 -3

Appendix 2 A 1. Turn power button on or observe the power button has Appendix 2 A 1. Turn power button on or observe the power button has been activated. If the EBAT device is in the STANDBY mode, press the START TEST button. Rev. 3. 09 9 -4

Appendix 2 A 2. The subject must remove foreign objects from the nose and Appendix 2 A 2. The subject must remove foreign objects from the nose and mouth including dentures. The subject must be closely and continuously observed for 20 minutes prior to testing to assure no belching, regurgitation or intake of any foreign material by nose or mouth has occurred. If such occurs, another 20 minutes of close and continuous observation must elapse under the same conditions. Start Time: Rev. 3. 09 Stop Time: 9 -5

Appendix 2 A 3. Verify that the external breath tube and simulator vapor tube Appendix 2 A 3. Verify that the external breath tube and simulator vapor tube are both warm. Simulator Vapor Tube External Breath Tube Rev. 3. 09 9 -6

Appendix 2 A 4. Observe the simulator temperature is between 33. 8 degrees Centigrade Appendix 2 A 4. Observe the simulator temperature is between 33. 8 degrees Centigrade and 34. 2 degrees Centigrade. Simulator Display Rev. 3. 09 9 -7

Appendix 2 A 5. Press the START TEST button. Rev. 3. 09 9 -8 Appendix 2 A 5. Press the START TEST button. Rev. 3. 09 9 -8

Appendix 2 A 6. Follow the instructions and sequence of events as they appear Appendix 2 A 6. Follow the instructions and sequence of events as they appear on the EBAT device display. Rev. 3. 09 9 -9

Appendix 2 A 7. Retain all printouts generated by the EBAT device with the Appendix 2 A 7. Retain all printouts generated by the EBAT device with the DUI packet. (i. e. Error message printouts) Rev. 3. 09 9 -10

Function Keys F 1: EBAT Printout Prints the last EBAT test record. Active when Function Keys F 1: EBAT Printout Prints the last EBAT test record. Active when the Intoxilyzer 5000 EN is in the Ready Mode and the printer buffer still contains the results of the last EBAT performed. Note: Evidential Breath Alcohol Test (EBAT) Rev. 3. 09 9 -11

Function Keys F 2: Print Simulator Log Prints the current simulator log. Active when Function Keys F 2: Print Simulator Log Prints the current simulator log. Active when the Intoxilyzer 5000 EN is in the Ready Mode. When depressed, it clears the printer buffer. The F 1 key will no longer print the last EBAT due to the printer buffer being cleared. Rev. 3. 09 9 -12

Function Keys F 5: Data Entry Return Key Returns the Intoxilyzer 5000 EN to Function Keys F 5: Data Entry Return Key Returns the Intoxilyzer 5000 EN to the Ready Mode when in a data entry operation. Is active only when in a data entry mode. Rev. 3. 09 9 -13

Function Keys F 6: Subject Non-Compliance Key Aborts the Test Sequence. C Is active Function Keys F 6: Subject Non-Compliance Key Aborts the Test Sequence. C Is active only from the end of the 2 nd Air Blank until the end of the 2 nd Breath Sample. Used only when the subject belches, regurgitates, or intakes a foreign material into their nose or mouth before the successful completion of the second breath test. Not to be used as a Refusal! Rev. 3. 09 9 -14

Stand By Mode The purpose the Stand By mode is to extend the life Stand By Mode The purpose the Stand By mode is to extend the life of critical electronic components in the Intoxilyzer 5000 EN. The power to the heater tapes is reduced during the Stand By mode. This allows the Sample Chamber, External Breath Tube, Internal Breath Tube, and Heated Simulator Tube to warm up quickly to their properating temperatures within ten minutes when the Start/Test Switch is depressed. The Stand By mode is automatically entered whenever the instrument is in-active for 120 minutes. Rev. 3. 09 9 -15

Stand By Mode Three indications that the instrument is in the Stand By Mode Stand By Mode Three indications that the instrument is in the Stand By Mode are: E The Intoxilyzer 5000 EN display is blank. E The red LED below the Power On button is lit. E The Guth 2100 Simulator displays ‘IDLE’. Rev. 3. 09 9 -16

Stand By Mode To bring the Intoxilyzer 5000 EN out of the Stand By Stand By Mode To bring the Intoxilyzer 5000 EN out of the Stand By mode and into the Ready mode, depress the Start/Test Switch. The instrument will display the following message: NOT READY And perform a short air purge Rev. 3. 09 9 -17

Stand By Mode The instrument will now display: WARM UP PERIOD XX: XX MST/MDT Stand By Mode The instrument will now display: WARM UP PERIOD XX: XX MST/MDT Current Time DATE XX/XX/XXXX Current Date Message repeats Rev. 3. 09 9 -18

Stand By Mode The ‘WARM UP’ period allows the Intoxilyzer 5000 EN and Guth Stand By Mode The ‘WARM UP’ period allows the Intoxilyzer 5000 EN and Guth 2100 Simulator to warm up to their properating temperatures. After the Warm Up period, the instrument will perform self diagnostic tests. These tests will ensure the Intoxilyzer 5000 EN is ready to accurately perform Evidential Breath Alcohol tests. Once all these tests have passed, the instrument will enter the Ready mode. Rev. 3. 09 9 -19

Diagnostic Tests During the diagnostic tests, numerous checks are performed to verify the properator Diagnostic Tests During the diagnostic tests, numerous checks are performed to verify the properator of the Intoxilyzer 5000 EN. Some of these tests are: TEMP CHECK Checks the temperature of the Sample Chamber to ensure it is between 45 C and 54 C. If this test fails, the instrument will pause the Diagnostic Tests and display the current temperature of the Sample Chamber. Once the temperature is correct, the Diagnostic Tests will continue. Rev. 3. 09 9 -20

Diagnostic Tests PRINTER CHECK Tests the interface between the instrument and printer. Printer error Diagnostic Tests PRINTER CHECK Tests the interface between the instrument and printer. Printer error information is displayed if detected. This error information will be displayed until the error is corrected (i. e. Printer offline, Out of Paper). DIAGNOSTIC OK Indicates all tests have passed. Rev. 3. 09 9 -21

Ready Mode CMI INC INTOXILYZER - ALCOHOL ANALYZER CO MODEL 5000 EN --- PUSH Ready Mode CMI INC INTOXILYZER - ALCOHOL ANALYZER CO MODEL 5000 EN --- PUSH BUTTON TO START TEST Scrolling PUSH BUTTON Flashing HH=MM MST/MDT DATE MM/DD/YY The above messages will continue to repeat until the Start Test Switch is depressed or after 120 minutes of inactivity and the Rev. 3. 09 instrument will go into the Stand By Mode. 9 -22

Data Entry Rev. 3. 09 9 -23 Data Entry Rev. 3. 09 9 -23

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A The test sequence of the Intoxilyzer 5000 EN is comprised of a series of steps or functions that ensure that when a error free print out is obtained the result reported is reliable and accurate. This is accomplished by performing numerous internal tests checking the operation of the Intoxilyzer. If the instrument detects an operation or result that is improper, it will generate the appropriate error message and abort the test sequence. Rev. 3. 09 9 -24

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A There are five functions that are performed during the test sequence. Each function executes specific operations as follows: A – Air Blank C – Calibration Check B – Breath Test I – Internal Standard Test 2 – Two minute wait period. Most of these functions are performs two or more times during the sequence. Rev. 3. 09 9 -25

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Air Blank There are seven Air Blanks performed during the test sequence. The primary purpose is to circulate room air through the sample chamber to remove any contaminates that may be present. This guarantees the sample chamber is clean prior to a Calibration Check, Breath Test, or Internal Standard Test. Rev. 3. 09 9 -26

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A 1 st Air Blank (continued). During an Air Blank, the instrument will display the following: AIR BLANK XX: XX MST/MDT DATE XX/XX/XXXX AIR BLANK Rev. 3. 09 9 -27

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A 1 st Air Blank (continued). Once complete, the instrument will display the result of the Air Blank. AIR BLANK. 000 Rev. 3. 09 9 -28

Test Sequence A C A B A I A 2 A B A CAL Test Sequence A C A B A I A 2 A B A CAL Check There are two calibration checks (CAL Check) performed during the test sequence. The primary function is to ensure the instrument is within calibration limits (± 10%). This is accomplished by re-circulating the vapor from the Guth 2100 Simulator through the sample chamber and back to the simulator. The re-circulation allows the utilization of the standard simulator solution for up to 96 subject tests. Rev. 3. 09 9 -29

Test Sequence A C A B A I A 2 A B A CAL Test Sequence A C A B A I A 2 A B A CAL Check During a CAL Check, the instrument will display: . . . . CAL. CHECK. ### At the completion of the second CAL Check, the instrument will compare the results of the two CAL Checks to ensure they are within 10% of each other. Rev. 3. 09 9 -30

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test The primary function of a Breath Test is to accurately determine the Br. AC of the subject. This is accomplished by the Beer-Lambert law. ‘Absorption is directly proportional to concentration. ’ The following basic formula is used for this calculation: X – Y = Alcohol Concentration (Br. AC) Were: X = Previsious Air Blank value (IR Concentration). Y = Current sample chamber value (IR Concentration). Rev. 3. 09 9 -31

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) The instrument will display the following at the start of a breath test. . . . PLEASE BLOW/R INTO MOUTHPIECE UNTIL TONE STOPS PLEASE BLOW/R Rev. 3. 09 9 -32

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) When the instrument displays ‘Please Blow/R’, the subject has approximately three minutes to provide a valid breath sample. If the subject does not provide a valid breath sample, a ‘Deficient Sample’ error message will be generated and the test sequence will be aborted. As long as the instrument displays ‘Please Blow/R’, the operator can initiate a ‘Refusal’ if the subject refuses to follow their instructions (“R” + on keyboard). Once the subject starts blowing, the instrument will display Rev. 3. 09 ‘Please Blow’ and the refusal option can no longer be 9 -33

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) Valid Breath Sample: There are four requirements for a successful breath sample. They are: 1. Flow Rate 2. Time 3. Volume 4. Slope Rev. 3. 09 9 -34

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) Valid Breath Sample 1. Flow Rate: The subject must blow into the mouth piece at a rate of ≥. 15 L / second. This minimum flow rate must be maintained until all four requirements are met. 2. Time: The subject must blow, at an acceptable rate, for a minimum of 1 second. Rev. 3. 09 9 -35

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) Valid Breath Sample 3. Volume: The subject must provide, at an acceptable rate, a minimum of 1. 1 Liters of breath sample. 4. Slope: Once the first three requirement are met, the Intoxilyzer will determine a level slope of the breath sample within the sample chamber. A level slope is when the Br. AC inside the sample chamber no longer increases by more than 3. 2% per second or deceases by more than 1. 6% per second. Rev. 3. 09 9 -36

Test Sequence A C A B A I A Typical Breath Sample: 2 A Test Sequence A C A B A I A Typical Breath Sample: 2 A B A C A Slope levels out at approximately 4 seconds Rising Slope during the first 4 seconds and the first three requirements are met. Subject Stops Blowing B r A C Level Slope Detected 0 Rev. 3. 09 1 2 3 4 5 6 Seconds 7 8 9 10 9 -37

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Mouth Alcohol: Positive Slope during the Slope peaks at approximately first 3 seconds. The first 3 seconds and should level 3 requirements are met. out. Slope then start to continually drop from the peak at a rate of ≥ 5%. B r A C 0 1 2 3 4 5 6 7 8 9 10 TIME Rev. 3. 09 9 -38

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) Instrument checks for interferent conditions. ( Acetone, Toluene, and other common alcohol. ) NOTE: If an interferent condition is detected, the instrument will display INTERF DETECTED. After a few seconds, if the instrument displays SUBTRACTED, it indicates to the operator that the interferent was Acetone has similar effects on the human brain as does Ethanol. A diabetic with an improper blood sugar level can naturally produce Acetone in their body, and in some cases exhibit the same mannerism as an individual who is intoxicated. The subject needs immediate medical assistance! Rev. 3. 09 9 -39

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) SUBJECT TEST. - - - The instrument will not display the results of the breath test. This helps in ensuring the subject will provide a second valid breath sample later in the test sequence. Rev. 3. 09 9 -40

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) Scrolling PLEASE REMOVE SPIT TRAP AND DEPRESS START TEST SWITCH REMOVE SPIT TRAP Flashing Operator must comply with the above instrument message within approximately 1 minute and 10 seconds. If not, a ‘Step Expired’ error message will occur and the test will be aborted. Rev. 3. 09 9 -41

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Breath Test (continued) At the completion of the 2 nd Breath Test, the two will be compared to ensure they are within 0. 02 of each other. If they are outside this limit, a ‘NO 02’ agreement error will be generated. Rev. 3. 09 9 -42

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A Internal Standard Test INTERNAL STD 1. This is a check of the instrument’s calibration and linearity using internal electronics values that equate to Br. AC values of 0. 100, 0. 200, and 0. 300. The results of all three Internal Standards must be within +/- 5% of the target values. 2. The addition of this step brackets the subject’s 1 st and 2 nd breath test with an instrument calibration and linearity check. 3. Will reduce challenges to the breath test accuracy by demonstrating the instrument was working properly after and before the subject provided the two breath samples. 4. Same Internal Standards test that is performed during the Diagnostic Checks. INTERNAL PASS Rev. 3. 09 9 -43

Test Sequence A C A B A I A 2 A B A C Test Sequence A C A B A I A 2 A B A C A 2 Minute Wait Period. WAIT 2 MINUTES WAIT 01. 56 Display counts down to zero WAIT 00. 00 1 second tone Allows the subject’s lungs to equilibrate to the 1: 2100 ratio based on Henry’s Law. Rev. 3. 09 9 -44

Ready Mode CMI INC INTOXILYZER - ALCOHOL ANALYZER CO MODEL 5000 EN --- PUSK Ready Mode CMI INC INTOXILYZER - ALCOHOL ANALYZER CO MODEL 5000 EN --- PUSK BUTTON TO START TEST Scrolling PUSH BUTTON Flashing HH=MM MST/MDT DATE MM/DD/YY The above messages will continue to repeat until the Start Test Switch is depressed or after 120 minutes of inactivity and the Rev. 3. 09 instrument will go into the Stand By Mode. 9 -45

EBAT PRINT OUT Header Data Entry Test Sequence Signature Rev. 3. 09 9 -46 EBAT PRINT OUT Header Data Entry Test Sequence Signature Rev. 3. 09 9 -46

EBAT PRINT OUT Header Serial Number provided by instrument. Rev. 3. 09 9 -47 EBAT PRINT OUT Header Serial Number provided by instrument. Rev. 3. 09 9 -47

EBAT PRINT OUT Data Entry SEQUENCE NUMBER: 00277 SUB NAME = DOE, JOHN, Q EBAT PRINT OUT Data Entry SEQUENCE NUMBER: 00277 SUB NAME = DOE, JOHN, Q SUB DOB = 11/24//90 ZIP CODE = 99999 SUB DRIV LIC = CO / 999 -99 -9999 OPER NAME = SMITH INTOX. RE-CERT DATE = 04/18/07 CASE NUMBER = 01724 SIM SOL NO = 11 -11 -11 SUB SEX = M SSN = 999 -99 -9999 AGENCY = CDPHE OPER CERT DATE = 12/11/07 COPY NO X OF XX Sequence Number, Intox Re-Cert Date, Copy No and Sim Sol No are provided by the instrument. Rev. 3. 09 9 -48

EBAT PRINT OUT Test Sequence Displays the test sequence along with the results of EBAT PRINT OUT Test Sequence Displays the test sequence along with the results of each step. Includes simulator temperatures and breath volumes. Times of the completion of each step are also printed. Reported value equals the lesser of the two independent breath samples. Rev. 3. 09 10 -49

EBAT PRINT OUT Signature Operator must sign the signature line of the EBAT printout EBAT PRINT OUT Signature Operator must sign the signature line of the EBAT printout Rev. 3. 09 9 -50

THE COMPLETED EBAT 1. 2. Rev. 3. 09 Complete and sign Appendix 2 A. THE COMPLETED EBAT 1. 2. Rev. 3. 09 Complete and sign Appendix 2 A. Sign EBAT printout. 9 -51

1. 2. 3. 4. 5. 6. 7. 8. Appendix 2 A Review Functions Key 1. 2. 3. 4. 5. 6. 7. 8. Appendix 2 A Review Functions Key Stand By Mode Warm Up Period Diagnostic Tests Ready Mode Test Sequence (ACABAIA 2 ABACA) EBAT Print Out Rev. 3. 09 9 -52