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THE BECKMAN COULTER logo, COULTER, Dx. H, Tru. Color, and Uni. Cel are trademarks THE BECKMAN COULTER logo, COULTER, Dx. H, Tru. Color, and Uni. Cel are trademarks of Beckman Coulter, Inc; COULTER and Uni. Cel are registered in the USPTO. Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 1

Objectives Software Overview System & Details Status Daily Checks & Shutdown System Maintenance Set-Up Objectives Software Overview System & Details Status Daily Checks & Shutdown System Maintenance Set-Up Sample Processing Cleaning Procedures Sample-Flow Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 2

Software Overview Status Area Utility Icons Alert Status Icons Navigation Icons System Icons Local Software Overview Status Area Utility Icons Alert Status Icons Navigation Icons System Icons Local Navigation Bar Rev. A (May 2012) Status Bar Professional Development © 2012 Beckman Coulter, Inc. 3

System Status LIS Status: Green = LIS Interface ON Gray = LIS Interface OFF System Status LIS Status: Green = LIS Interface ON Gray = LIS Interface OFF DATE & TIME of last DAILY CHECK & SHUTDOWN Cycles Last Control Run Last Calibration Change Default Test Order Automatic: Specimen - Cassette Manual: Specimen - Single-Tube Rev. A (May 2012) Printer Status: Displays Auto Print Disabled if Disable All Auto Printed Reports is selected from Printer Setup Professional Development © 2012 Beckman Coulter, Inc. 4

Daily Checks & Shutdown Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Daily Checks & Shutdown Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 5

Supplies Diluent 1 & 2 Cleaner Diff Pack Retic Pack Cell Lyse Waste Status Supplies Diluent 1 & 2 Cleaner Diff Pack Retic Pack Cell Lyse Waste Status Edit Dx. H Setup Supplies Configure Container Rev. A (May 2012) Configure Low Level View Log Refresh Professional Development © 2012 Beckman Coulter, Inc. 6

Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 7 Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 7

Operators and Roles Menu>Setup>Operators and Roles>New Operator Rev. A (May 2012) Professional Development © Operators and Roles Menu>Setup>Operators and Roles>New Operator Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 8

Control Files Menu>Setup>Quality Control>Control Tab New Control from Bar Code Rev. A (May 2012) Control Files Menu>Setup>Quality Control>Control Tab New Control from Bar Code Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 9

Calibration Menu>QA>CBC Calibration Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. Calibration Menu>QA>CBC Calibration Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 10

Flagging Limits Menu> Setup> Flagging/Rules> Flagging Limits Tab Rev. A (May 2012) Professional Development Flagging Limits Menu> Setup> Flagging/Rules> Flagging Limits Tab Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 11

Flagging Sensitivity Menu> Setup> Flagging/Rules> Flagging Sensitivity Tab Rev. A (May 2012) Professional Development Flagging Sensitivity Menu> Setup> Flagging/Rules> Flagging Sensitivity Tab Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 12

Decision Rules Menu>Setup>Flagging/Rules>Release Rules Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Decision Rules Menu>Setup>Flagging/Rules>Release Rules Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 13

Decision Rules Menu>Setup>Flagging/Rules>Flags>Decision Rules Tab Decision Rules Add Rule Rev. A (May 2012) Restore Decision Rules Menu>Setup>Flagging/Rules>Flags>Decision Rules Tab Decision Rules Add Rule Rev. A (May 2012) Restore Consensus Rules Professional Development © 2012 Beckman Coulter, Inc. 14

Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 15 Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 15

Running Samples Cassette Presentation • Load the samples into the cassettes • Place cassettes Running Samples Cassette Presentation • Load the samples into the cassettes • Place cassettes into the input buffer Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 16

Running Samples Single Tube Presentation Rev. A (May 2012) Professional Development © 2012 Beckman Running Samples Single Tube Presentation Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 17

Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 18 Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 18

Dx. H 800 Cleaning Procedures ¬ Daily Checks ¬ Shutdown ¬ Process Controls ¬ Dx. H 800 Cleaning Procedures ¬ Daily Checks ¬ Shutdown ¬ Process Controls ¬ As Needed Procedures ¬ For additional information on As Needed Procedures refer to Instructions For Use (IFU) Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 19

Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 20 Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 20

Sample Transport Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 21 Sample Transport Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 21

Mixing Station Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 22 Mixing Station Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 22

Sample Aspiration Module (SAM) Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Sample Aspiration Module (SAM) Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 23

CBC Module Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 24 CBC Module Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 24

CBC Module Sweep-Flow & Tangential Mixing Sweep Flow Rev. A (May 2012) Tangential Mixing CBC Module Sweep-Flow & Tangential Mixing Sweep Flow Rev. A (May 2012) Tangential Mixing Professional Development © 2012 Beckman Coulter, Inc. 25

NRBC Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 26 NRBC Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 26

Differential Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 27 Differential Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 27

Retics Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 28 Retics Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 28

Any Questions? Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 29 Any Questions? Rev. A (May 2012) Professional Development © 2012 Beckman Coulter, Inc. 29