MSU & SkolTech Phage and virus replication Phage

MSU & SkolTech Phage and virus replication

Phage and virus replication Extreme diversity of virus genetic material and its transformations

Phage l Linear double stranded DNA genome, able to circularize

Phage l Transition from  to rolling cycle mechanism of replication

Phage l Genomes separation is coupled to packaging

Phage l Lysogenic pathway: integration of phage genome to the host genome

Phage l Integration of phage genome to the host genome via covalent intermediate

Phage l Integration of phage genome to the host genome via covalent intermediate

Phage l Intasome – DNA/protein complex

Phage l Phage genome could be excised, but it requires additional protein, Xis

Phage fX174 Circular single stranded DNA genome, able to form circular double stranded form

Phage fX174 Circular single stranded DNA genome, able to form circular double stranded form

Phage fX174 Start of the (+) strand synthesis

Phage fX174 Single stranded variant of the rolling cycle replication

Phage Mu A transposon-like phage

Phage Mu Integration to the host genome

Phage Mu Phage Mu behaves as a replicative transposon within the host genome

Phage Mu For phage particle assembly a phage genome is cutted from the host genome with excess DNA

Retrovirus Linear single stranded (+) RNA genome, able to form linear double stranded integrated DNA form

Retrovirus Problem of LTR formation

Retrovirus LTR is necessary for transcription of the genome

Influenza virus Linear single stranded (-) RNA genome, able to form linear double stranded RNA genome and single stranded (+) form

Influenza virus Stealing the cap from host mRNA

Influenza virus Unusual mechanism of polyadenylation

Influenza virus Replication doesn’t require caps of host mRNA