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FSS-i 3 DNA expert system suite FSS-i 3 DNA expert system suite

DNA Profiling • Sample e. g. hair, skin DNA Profiling • Sample e. g. hair, skin

DNA Profiling • Sample e. g. hair, skin • Process sample to obtain raw DNA Profiling • Sample e. g. hair, skin • Process sample to obtain raw data

DNA Profiling • Sample e. g. hair, skin • Process sample to obtain raw DNA Profiling • Sample e. g. hair, skin • Process sample to obtain raw data • Produce a profile of the DNA

DNA Profiling • Sample e. g. hair, skin • Process sample to obtain raw DNA Profiling • Sample e. g. hair, skin • Process sample to obtain raw data • Produce a profile of the DNA • Store profile in NDIS (National DNA Index System) or match to existing profile

Expert System profiling motivation • Sample & data processing already heavily automated Expert System profiling motivation • Sample & data processing already heavily automated

Expert System profiling motivation • Sample & data processing already heavily automated • Sample Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck.

Expert System profiling motivation • Sample & data processing already heavily automated • Sample Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck. • Two independent data reviews required to upload a profile to NDIS

Expert System profiling motivation • Sample & data processing already heavily automated • Sample Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck. • Two independent data reviews required to upload a profile to NDIS • Save enormous amounts of review time

FSS-i 3 Expert System • FSS: Forensic Science Service of the UK FSS-i 3 Expert System • FSS: Forensic Science Service of the UK

FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i 3 – 6 years of R&D and validation

FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i 3 – 6 years of R&D and validation • Designed by forensic scientists

FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i 3 – 6 years of R&D and validation • Designed by forensic scientists • Made available to forensic community – USA, Canada, Europe, Australia, New Zealand

FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed FSS-i 3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i 3 – 6 years of R&D and validation • Designed by forensic scientists • Made available to forensic community – USA, Canada, Europe, Australia, New Zealand • Full Audit system

i-STRess • The core DNA interpretation tool i-STRess • The core DNA interpretation tool

i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using configurable rules & filters

i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using configurable rules & filters • Detect multi-person DNA mixtures within a profile & export to i-STReam…

i-STReam • Unique functionality to resolve two person DNA mixtures i-STReam • Unique functionality to resolve two person DNA mixtures

i-STReam • Unique functionality to resolve two person DNA mixtures • Allows previously unusable i-STReam • Unique functionality to resolve two person DNA mixtures • Allows previously unusable DNA profiles to be uploaded to DNA databases

i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture

i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible profile contaminations for further review

i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible profile contaminations for further review • Saves time: no more manual reviews of profiles

FSS-i 3 Validation • FSS-i 3 developed from internal FSS expert systems – 6 FSS-i 3 Validation • FSS-i 3 developed from internal FSS expert systems – 6 years of testing

FSS-i 3 Validation • FSS-i 3 developed from internal FSS expert systems – 6 FSS-i 3 Validation • FSS-i 3 developed from internal FSS expert systems – 6 years of testing • 1 million+ profiles uploaded to UK National DNA DB

FSS-i 3 Validation • FSS-i 3 developed from internal FSS expert systems – 6 FSS-i 3 Validation • FSS-i 3 developed from internal FSS expert systems – 6 years of testing • 1 million+ profiles uploaded to UK National DNA DB • FSS-i 3 validation project: – 70 000 previously analyzed samples – Minor discrepancies in 0. 03% of samples & each of these samples were flagged by the system for further review

Technology used in FSS-i 3 • Strategies for implementing rules: unknown Technology used in FSS-i 3 • Strategies for implementing rules: unknown

Technology used in FSS-i 3 • Strategies for implementing rules: unknown • User optimization: Technology used in FSS-i 3 • Strategies for implementing rules: unknown • User optimization: 20+ rules settings

Technology used in FSS-i 3 • Strategies for implementing rules: unknown • User optimization: Technology used in FSS-i 3 • Strategies for implementing rules: unknown • User optimization: 20+ rules settings • Designed to run on stand-alone Pentium 3 PC’s

Design & Development of FSS-i 3 • Development time: six years Design & Development of FSS-i 3 • Development time: six years

Design & Development of FSS-i 3 • Development time: six years • “The FSS Design & Development of FSS-i 3 • Development time: six years • “The FSS developers who designed the FSS-i 3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1)

Design & Development of FSS-i 3 • Development time: six years • “The FSS Design & Development of FSS-i 3 • Development time: six years • “The FSS developers who designed the FSS-i 3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1) • “i-STReam is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton” (1)

Conclusions: FSS-i 3 • Consistent profiling of samples Conclusions: FSS-i 3 • Consistent profiling of samples

Conclusions: FSS-i 3 • Consistent profiling of samples • Ability to interpret mixed DNA Conclusions: FSS-i 3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles

Conclusions: FSS-i 3 • Consistent profiling of samples • Ability to interpret mixed DNA Conclusions: FSS-i 3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles • Check for contamination

Conclusions: FSS-i 3 • Consistent profiling of samples • Ability to interpret mixed DNA Conclusions: FSS-i 3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles • Check for contamination • Significant reduction in time spent on the above, due to automation

References • (1) http: //www. forensic. gov. uk/forensic_t/i 3/index. htm • (2) http: //www. References • (1) http: //www. forensic. gov. uk/forensic_t/i 3/index. htm • (2) http: //www. promega. com/profiles/802/profilesindna_802_08. pdf • (3) http: //forensics. marshall. edu/NEST/Nest%20 PDFs/Documents/NIJEval. Expe rt. Sys. pdf • (4) http: //www. promega. com/profiles/1001/profilesindna_1001_11. pdf • (5) http: //www. kathyreichs. com/dnaprofiling. htm • (6) http: //www. biotechnologyonline. gov. au/human/dnaprofile. cfm