Скачать презентацию Cardiac-Specific Overexpression of Fibroblast Growth Factor-2 Protects Against Скачать презентацию Cardiac-Specific Overexpression of Fibroblast Growth Factor-2 Protects Against

025c2850afd93b2b77ec593206286651.ppt

  • Количество слайдов: 7

Cardiac-Specific Overexpression of Fibroblast Growth Factor-2 Protects Against Myocardial Dysfunction and Infarction in a Cardiac-Specific Overexpression of Fibroblast Growth Factor-2 Protects Against Myocardial Dysfunction and Infarction in a Murine Model of Low-Flow Ischemia by Stacey L. House, Craig Bolte, Ming Zhou, Thomas Doetschman, Raisa Klevitsky, Gilbert Newman, and Jo El J. Schultz Circulation Volume 108(25): 3140 -3148 December 23, 2003 Copyright © American Heart Association, Inc. All rights reserved.

Figure 1. Schematic of low-flow ischemia protocols (A, 30 minutes of ischemia and 30 Figure 1. Schematic of low-flow ischemia protocols (A, 30 minutes of ischemia and 30 minutes of reperfusion to measure reversible injury; B, 60 minutes of ischemia and 120 minutes of reperfusion to measure irreversible injury). Stacey L. House et al. Circulation. 2003; 108: 3140 -3148 Copyright © American Heart Association, Inc. All rights reserved.

Figure 2. Western blot analysis of FGF 2 protein expression. Stacey L. House et Figure 2. Western blot analysis of FGF 2 protein expression. Stacey L. House et al. Circulation. 2003; 108: 3140 -3148 Copyright © American Heart Association, Inc. All rights reserved.

Figure 3. Recovery of contractile function as measured by +d. P/dt in Wt and Figure 3. Recovery of contractile function as measured by +d. P/dt in Wt and Fgf 2 KO hearts (A) and Wt and FGF 2 Tg hearts (B). Stacey L. House et al. Circulation. 2003; 108: 3140 -3148 Copyright © American Heart Association, Inc. All rights reserved.

Figure 4. Representative Masson’s trichrome images of sham-treated Wt (A and C), Fgf 2 Figure 4. Representative Masson’s trichrome images of sham-treated Wt (A and C), Fgf 2 KO (B), and FGF 2 Tg (D) hearts and Wt (E and G), Fgf 2 KO (F), and FGF 2 Tg (H) hearts after 60 minutes of ischemia and 120 minutes of reperfusion. Stacey L. House et al. Circulation. 2003; 108: 3140 -3148 Copyright © American Heart Association, Inc. All rights reserved.

Figure 5. Infarct size expressed as a percentage of the area at risk in Figure 5. Infarct size expressed as a percentage of the area at risk in Wt (A, black bar) and Fgf 2 KO (A, dark gray bar) hearts and Wt (B, black bar), FGF 2 Tg MHC 20 (B, gray bar), and FGF 2 Tg MHC 25 (B, white bar). Stacey L. House et al. Circulation. 2003; 108: 3140 -3148 Copyright © American Heart Association, Inc. All rights reserved.

Figure 6. FGF 2 concentration measured in coronary perfusate from Fgf 2 KO hearts Figure 6. FGF 2 concentration measured in coronary perfusate from Fgf 2 KO hearts (A) or FGF 2 Tg hearts (B). Stacey L. House et al. Circulation. 2003; 108: 3140 -3148 Copyright © American Heart Association, Inc. All rights reserved.